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CNAP1 Antibody

Images

 
Genetic Strategies: Western Blot: CNAP1 Antibody [NBP1-88201] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunohistochemistry: CNAP1 Antibody [NBP1-88201] - Staining of human tonsil shows strong cytoplasmic and nuclear positivity in reaction center cells.
Western Blot: CNAP1 Antibody [NBP1-88201] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 High

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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CNAP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DKSVLVCKNAIQLLASFLANNPFSCKLSDADLAGPLQKETQKLQEMRAQRRTAAASAVLDPEEEWEAMLPELKSTLQQLLQLPQGEEEIPEQIA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCAPD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CNAP1 Protein (NBP1-88201PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CNAP1 Antibody

  • CAPD2
  • CAP-D2
  • Chromosome condensation-related SMC-associated protein 1
  • Chromosome-associated protein D2
  • CNAP1XCAP-D2 homolog
  • hCAP-D2condensin complex subunit 1
  • KIAA0159chromosome condensation related SMC associated protein 1
  • Non-SMC condensin I complex subunit D2
  • non-SMC condensin I complex, subunit D2

Background

CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-88201

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Bioinformatics

Gene Symbol NCAPD2