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CMAS Antibody (5A2)

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Western Blot: CMAS Antibody (5A2) [H00055907-M01] - CMAS monoclonal antibody (M01), clone 5A2. Analysis of CMAS expression in Raw 264.7.
Western Blot: CMAS Antibody (5A2) [H00055907-M01] - CMAS monoclonal antibody (M01), clone 5A2. Analysis of CMAS expression in Raw 264.7.
Immunocytochemistry/ Immunofluorescence: CMAS Antibody (5A2) [H00055907-M01] - Analysis of monoclonal antibody to CMAS on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CMAS Antibody (5A2) [H00055907-M01] - Analysis of monoclonal antibody to CMAS on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
Western Blot: CMAS Antibody (5A2) [H00055907-M01] - CMAS monoclonal antibody (M01), clone 5A2. Analysis of CMAS expression in PC-12.
Western Blot: CMAS Antibody (5A2) [H00055907-M01] - CMAS monoclonal antibody (M01), clone 5A2 Analysis of CMAS expression in Hela S3 NE.
Western Blot: CMAS Antibody (5A2) [H00055907-M01] - CMAS monoclonal antibody (M01), clone 5A2. Analysis of CMAS expression in NIH/3T3.
Sandwich ELISA: CMAS Antibody (5A2) [H00055907-M01] - Detection limit for recombinant GST tagged CMAS is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
5A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CMAS Antibody (5A2) Summary

Immunogen
CMAS (NP_061156, 164 a.a. ~ 263 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLR
Specificity
CMAS - cytidine monophosphate N-acetylneuraminic acid synthetase
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CMAS
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluorescence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human, mouse & rat. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CMAS Antibody (5A2)

  • CMP-N-acetylneuraminic acid synthase
  • CMP-N-acetylneuraminic acid synthetase
  • CMP-Neu5Ac synthetase
  • CMP-NeuNAc synthase
  • CMP-NeuNAc synthetase
  • cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
  • cytidine monophosphate N-acetylneuraminic acid synthetase
  • EC 2.7.7.43
  • N-acylneuraminate cytidylyltransferase

Background

The enzyme encoded by this gene catalyzes the activation of Neu5Ac to Cytidine 5-prime-monophosphate N-acetylneuraminic acid (CMP-Neu5Ac), which provides the substrate required for the addition of sialic acid. Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is highly regulated during embryonic development, and changes with stages of differentiation. Studies of a similar murine protein suggest that this protein localizes to the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00055907-M01
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Product General Protocols

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Bioinformatics

Gene Symbol CMAS
Entrez
OMIM
Uniprot