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CLOCK Antibody

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Western blot shows lysates of PC-3 human prostate cancer cell line and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Human CLOCK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4026) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CLOCK at approximately 95 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Western blot shows lysates of PC-3 human prostate cancer cell line and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Human CLOCK Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4026-SP
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CLOCK Antibody Summary

Immunogen
E. coli-derived recombinant human CLOCK
Glu108-Glu234
Accession # O15516
Specificity
Detects human CLOCK in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CLOCK
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLOCK Antibody

  • BHLHE8
  • circadian locomoter output cycles protein kaput
  • Class E basic helix-loop-helix protein 8
  • clock (mouse) homolog
  • clock homolog (mouse)
  • CLOCK
  • EC 2.3.1.48
  • hCLOCK
  • KAT13D
  • KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Background

CLOCK is an essential regulator of circadian rhythms. CLOCK is a basic helix-loop-helix-PAS transcription factor that heterodimerizes with BMAL1. Together these transcription factors bind to E box elements (CACGTG) within the promoter of circadian genes (period1 and 2) stimulating their transcription. BMAL dimerization is necessary for CLOCK’s nuclear translocation, E box dependent transcription, phosphorylation, and degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol CLOCK
Uniprot