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TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

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Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) ...read more
Exogenous biological renal support improved renal IRI and decreased mortality and serum Cr, BUN levels in old IRI mice. (A) Survival curves for the old IRI mice at 72 hours. (B) Cr levels in the old mice. (C) BUN levels ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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TIM-1/KIM-1/HAVCR Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
Tyr22-Thr212
Accession # NP_001160104
Specificity
Detects mouse TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HAVCR1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF1817 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

  • CD365
  • HAVCR1
  • HAVCR-1
  • HAVCRT cell immunoglobin domain and mucin domain protein 1
  • hepatitis A virus cellular receptor 1
  • Kidney injury molecule 1
  • KIM1
  • KIM-1
  • T-cell immunoglobulin and mucin domain-containing protein 1
  • TIM1
  • TIM-1
  • TIM-1TIM
  • TIM1TIMD-1
  • TIMD1T-cell membrane protein 1

Background

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. 
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
  4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.  
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113. 
  6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  8. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIM-1/KIM-1/HAVCR Antibody (AF1817)(105)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(2)
IHC
(24)
IHC-Fr
(2)
IHC-P
(2)
Immunohistochemistry
(7)
Western Blot
(26)
All Applications
Filter By Species
Human
(5)
Mouse
(52)
Transgenic Mouse
(4)
All Species
Showing Publications 1 - 10 of 105. Show All 105 Publications.
Publications using AF1817 Applications Species
Hirashima, Y;Nakano, T;Torisu, K;Aihara, S;Wakisaka, M;Kitazono, T; SGLT2 inhibition mitigates transition from acute kidney injury to chronic kidney disease by suppressing ferroptosis Scientific reports 2024-09-02 [PMID: 39223189] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Hammouri, D;Orwick, A;Doll, M;Vega, DS;Shah, PP;Clarke, CJ;Clem, B;Beverly, LJ;Siskind, LJ; Remote organ cancer adversely alters renal function and induces kidney injury, inflammation, and fibrosis bioRxiv : the preprint server for biology 2024-07-30 [PMID: 39211238] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Huang, XR;Ye, L;An, N;Wu, CY;Wu, HL;Li, HY;Huang, YH;Ye, QR;Liu, MD;Yang, LW;Liu, JX;Tang, JX;Pan, QJ;Wang, P;Sun, L;Xia, Y;Lan, HY;Yang, C;Liu, HF; Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1 Autophagy 2024-08-28 [PMID: 39193910] (Immunohistochemistry, Western Blot, Mouse) Immunohistochemistry, Western Blot Mouse
Biniaris-Georgallis, SI;Aschman, T;Stergioula, K;Schreiber, F;Jafari, V;Taranko, A;Karmalkar, T;Kasapi, A;Lenac Rovis, T;Jelencic, V;Bejarano, DA;Fabry, L;Papacharalampous, M;Mattiola, I;Molgora, M;Hou, J;Hublitz, KW;Heinrich, F;Guerra, GM;Durek, P;Patone, G;Lindberg, EL;Maatz, H;Hölsken, O;Krönke, G;Mortha, A;Voll, RE;Clarke, AJ;Hauser, AE;Colonna, M;Thurley, K;Schlitzer, A;Schneider, C;Stamatiades, EG;Mashreghi, MF;Jonjic, S;Hübner, N;Diefenbach, A;Kanda, M;Triantafyllopoulou, A; Amplification of autoimmune organ damage by NKp46-activated ILC1 Nature 2024-08-13 [PMID: 39137897] (Immunohistochemistry, Mouse, Transgenic Mouse) Immunohistochemistry Mouse, Transgenic Mouse
Pan, Y;Peng, Z;Fang, Z;Iddrisu, L;Sun, L;Deng, Q;Gooneratne, R; A Tripeptide (Ser-Arg-Pro, SRP) from Sipunculus nudus L. Improves Cadmium-Induced Acute Kidney Injury by Targeting the MAPK, Inflammatory, and Apoptosis Pathways in Mice Marine drugs 2024-06-20 [PMID: 38921597] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Noonan, ML;Muto, Y;Yoshimura, Y;Leckie-Harre, A;Wu, H;Kalinichenko, VV;Humphreys, BD;Chang-Panesso, M; Injury-induced FoxM1 expression in mouse kidney drives epithelial proliferation by a Cyclin F dependent mechanism JCI insight 2024-06-25 [PMID: 38916959] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Wang, P;Ouyang, J;Zhou, K;Hu, D;Zhang, S;Zhang, A;Yang, Y; Olesoxime protects against cisplatin-induced acute kidney injury by attenuating mitochondrial dysfunction Biomedical journal 2024-04-19 [PMID: 38643825] (Western Blot, Mouse) Western Blot Mouse
Diaz-Bulnes, P;Rodríguez, RM;Banon-Maneus, E;Saiz, ML;Bernet, CR;Corte-Iglesias, V;Ramirez-Bajo, MJ;Lazo-Rodriguez, M;Tamargo-Gómez, I;Rodrigues-Diez, RR;Sanz, AB;Diaz-Corte, C;Ruiz-Ortega, M;Diekmann, F;Aransay, AM;Lopez-Larrea, C;Suarez-Alvarez, B; Inhibition of BRD4 Attenuates ER Stress-induced Renal Ischemic-Reperfusion Injury International journal of biological sciences 2024-02-11 [PMID: 38481808] (Western Blot, Mouse) Western Blot Mouse
Vlasschaert, C;Robinson-Cohen, C;Chen, J;Akwo, E;Parker, AC;Silver, SA;Bhatraju, PK;Poisner, H;Cao, S;Jiang, M;Wang, Y;Niu, A;Siew, E;Van Amburg, JC;Kramer, HJ;Kottgen, A;Franceschini, N;Psaty, BM;Tracy, RP;Alonso, A;Arking, DE;Coresh, J;Ballantyne, CM;Boerwinkle, E;Grams, M;Zhang, MZ;Kestenbaum, B;Lanktree, MB;Rauh, MJ;Harris, RC;Bick, AG; Clonal hematopoiesis of indeterminate potential is associated with acute kidney injury Nature medicine 2024-03-01 [PMID: 38454125] (Western Blot, Mouse) Western Blot Mouse
Li, Z;He, R;Liu, J;Jin, X;Jiang, B;Lao, Y;Yang, S; JianPiYiShen formula prevents cisplatin-induced acute kidney injury in mice by improving necroptosis through MAPK pathway BMC complementary medicine and therapies 2024-02-24 [PMID: 38402163] (Western Blot, Mouse) Western Blot Mouse
Show All 105 Publications.

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Bioinformatics

Gene Symbol HAVCR1
Uniprot