![Western Blot: CKIP-1 Antibody [NBP1-76238] - Human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/CKIP-1-Antibody-Western-Blot-NBP1-76238-img0001.jpg "Western Blot: CKIP-1 Antibody [NBP1-76238] - Human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 ug/mL.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human CKIP1. The immunogen is located within the last 50 amino acids of CKIP1. Amino Acid Squence: TPDSHLRQTTPHSQYKKS |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLEKHO1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
| Publication using NBP1-76238 | Applications | Species |
|---|---|---|
| Piacentino M. L. , Bronner M. E. Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction. PLoS Biol 2018-06-01 [PMID: 29949573] (WB, Chicken) | WB | Chicken |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: CKIP-1 Antibody - BSA Free [NBP1-76238] - CKIP-1 directly interacts with Smurf1 to promote Smurf1 degradation.DF-1 chicken fibroblasts were transfected with the indicated constructs & then immunostained using anti-FLAG & anti-V5 antibodies. (A) Localization of FLAG-tagged CKIP-1 & V5-tagged Smurf1 alone. (B) Schematics for CKIP-1 FLAG constructs highlighting the membrane-binding PH domain (red) & Smurf1-binding LZ (blue) followed by a C-terminal FLAG tag (yellow). (C) Full-length CKIP-1 FLAG was cotransfected with full-length V5-Smurf1 or V5-Smurf1 C700A dominant-negative mutant. In addition, CKIP-1 FLAG truncation mutants were cotransfected with full-length V5-Smurf1, showing that the PH domain is required for efficient membrane localization, & the LZ domain is required for Smurf1 colocalization. Arrows indicate membrane localization. Scale bars represent 10 μm. (D) Chick embryos were electroporated at HH4 with V5-Smurf1 & CKIP-1 FLAG & then stained for V5 or FLAG at HH8 & imaged in cryosection. Scale bars represent 25 μm. (E) Western blot analysis of HH7 whole-embryo lysates electroporated with constructs indicated & probed for V5, pSmad1/5/8, & RFP. Bar graphs represent normalized V5 & pSmad1/5/8 staining intensity means ± SEM from 3 replicates. Underlying data can be found in S1 Data. P values from two-tailed Student t test. CKIP-1, casein kinase interacting protein 1; HH, Hamburger-Hamilton stage; LZ, leucine zipper motif; NC; neural crest domain; NS, not significant; PH, pleckstrin homology; pSmad1/5/8, phospho-Smads 1/5/8; RFP, red fluorescent protein; Smurf1, Smad ubiquitin regulatory factor 1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29949573), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76238_rabbit-polyclonal-ckip-1-antibody-310202416173526.jpg "Western Blot: CKIP-1 Antibody - BSA Free [NBP1-76238] - CKIP-1 directly interacts with Smurf1 to promote Smurf1 degradation.DF-1 chicken fibroblasts were transfected with the indicated constructs & then immunostained using anti-FLAG & anti-V5 antibodies. (A) Localization of FLAG-tagged CKIP-1 & V5-tagged Smurf1 alone. (B) Schematics for CKIP-1 FLAG constructs highlighting the membrane-binding PH domain (red) & Smurf1-binding LZ (blue) followed by a C-terminal FLAG tag (yellow). (C) Full-length CKIP-1 FLAG was cotransfected with full-length V5-Smurf1 or V5-Smurf1 C700A dominant-negative mutant. In addition, CKIP-1 FLAG truncation mutants were cotransfected with full-length V5-Smurf1, showing that the PH domain is required for efficient membrane localization, & the LZ domain is required for Smurf1 colocalization. Arrows indicate membrane localization. Scale bars represent 10 μm. (D) Chick embryos were electroporated at HH4 with V5-Smurf1 & CKIP-1 FLAG & then stained for V5 or FLAG at HH8 & imaged in cryosection. Scale bars represent 25 μm. (E) Western blot analysis of HH7 whole-embryo lysates electroporated with constructs indicated & probed for V5, pSmad1/5/8, & RFP. Bar graphs represent normalized V5 & pSmad1/5/8 staining intensity means ± SEM from 3 replicates. Underlying data can be found in S1 Data. P values from two-tailed Student t test. CKIP-1, casein kinase interacting protein 1; HH, Hamburger-Hamilton stage; LZ, leucine zipper motif; NC; neural crest domain; NS, not significant; PH, pleckstrin homology; pSmad1/5/8, phospho-Smads 1/5/8; RFP, red fluorescent protein; Smurf1, Smad ubiquitin regulatory factor 1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29949573), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")