![Western Blot: GilZ Antibody (3A5) [H00001831-M01] - Analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody (M01), clone 3A5.Lane 1: TSC22D3 transfected lysate(22.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GilZ-Antibody-3A5-Western-Blot-H00001831-M01-img0012.jpg "Western Blot: GilZ Antibody (3A5) [H00001831-M01] - Analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody (M01), clone 3A5.Lane 1: TSC22D3 transfected lysate(22.2 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clone | 3A5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | TSC22D3 (NP_932174, 1 a.a. ~ 97 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQSKLDCRSPVGLDCCNCCLDLAHRSGLQRGSSGENNNPGSPTVSNFRQLQEKLVFENLNTDKLNSIMRQDSLEPVLRDPCYLINEGICNRNIDQT |
| Specificity | TSC22D3 - TSC22 domain family, member 3 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TSC22D3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, IF and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Publication using H00001831-M01 | Applications | Species |
|---|---|---|
| Williamson M, Garg R, Wells C et al. PlexinB1 Promotes Nuclear Translocation of the Glucocorticoid Receptor. Cells. 2019-12-18 [PMID: 31861264] |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Santhosh Sivajothi |
IHC-P | Human | 09/25/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for GilZ Antibody (H00001831-M01)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: GilZ Antibody (3A5) [H00001831-M01] - Analysis of monoclonal antibody to TSC22D3 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GilZ-Antibody-3A5-Immunocytochemistry-Immunofluorescence-H00001831-M01-img0007.jpg "Immunocytochemistry/Immunofluorescence: GilZ Antibody (3A5) [H00001831-M01] - Analysis of monoclonal antibody to TSC22D3 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01] - Human lymph node. Antigen retrieval in citrate buffer at 95C for 30 min. Image from verified customer review.](http://images.novusbio.com/fullsize/GilZ-Antibody-3A5-Immunohistochemistry-Paraffin-H00001831-M01-img0013.jpg "Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01] - Human lymph node. Antigen retrieval in citrate buffer at 95C for 30 min. Image from verified customer review.")
![Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01] - Analysis of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/GilZ-Antibody-3A5-Immunohistochemistry-Paraffin-H00001831-M01-img0009.jpg "Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01] - Analysis of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.")
![Immunoprecipitation: GilZ Antibody (3A5) [H00001831-M01] - Analysis of TSC22D3 transfected lysate using anti-TSC22D3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TSC22D3 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/GilZ-Antibody-3A5-Immunoprecipitation-H00001831-M01-img0010.jpg "Immunoprecipitation: GilZ Antibody (3A5) [H00001831-M01] - Analysis of TSC22D3 transfected lysate using anti-TSC22D3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TSC22D3 MaxPab rabbit polyclonal antibody.")
![ELISA: GilZ Antibody (3A5) [H00001831-M01] - Detection limit for recombinant GST tagged TSC22D3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GilZ-Antibody-3A5-ELISA-H00001831-M01-img0011.jpg "ELISA: GilZ Antibody (3A5) [H00001831-M01] - Detection limit for recombinant GST tagged TSC22D3 is approximately 0.03ng/ml as a capture antibody.")
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