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CHCHD3 Antibody

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Immunoprecipitation: CHCHD3 Antibody [NBP3-38566] - Immunoprecipitation analysis of 600 ug extracts of Mouse liver cells using 3 ug CHCHD3 antibody. Western blot was performed from the immunoprecipitate using CHCHD3 ...read more
Immunocytochemistry/ Immunofluorescence: CHCHD3 Antibody [NBP3-38566] - Confocal immunofluorescence analysis of U2OS cells using CHCHD3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: CHCHD3 Antibody [NBP3-38566] - Western blot analysis of various lysates using CHCHD3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CHCHD3 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1).

Sequence:
MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHCHD3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunoprecipitation 0.5ug-4ug antibody for 400ug-600ug extracts of whole cells
  • Western Blot 1:500 - 1:2000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CHCHD3 Antibody

  • coiled-coil-helix-coiled-coil-helix domain containing 3
  • FLJ20420
  • mitochondrial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CHCHD3