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![Western Blot: CHCHD3 Antibody [NBP1-83656] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP1-83656-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CHCHD3 Antibody [NBP1-83656] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CHCHD3 Antibody [NBP1-83656] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83656-img0011.jpg)
![Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-83656] - Staining of human skeletal muscle shows strong granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP1-83656-img0015.jpg)
![Western Blot: CHCHD3 Antibody [NBP1-83656] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP1-83656-img0009.jpg)
![Western Blot: CHCHD3 Antibody [NBP1-83656] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP1-83656-img0010.jpg)
![Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-83656] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP1-83656-img0012.jpg)
![Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-83656] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP1-83656-img0013.jpg)
![Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-83656] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP1-83656-img0014.jpg)
![Western Blot: CHCHD3 Antibody [NBP2-21606] - Wild-type (WT) and CHCHD3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHCHD3 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP2-21606-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CHCHD3 Antibody [NBP2-21606] - Wild-type (WT) and CHCHD3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHCHD3 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP2-21606] - CHCHD3 antibody detects CHCHD3 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CHCHD3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP2-21606-img0004.jpg)
![Western Blot: CHCHD3 Antibody [NBP2-21606] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP2-21606-img0001.jpg)
![Western Blot: CHCHD3 Antibody [NBP2-21606] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP2-21606-img0002.jpg)
![Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP2-21606] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP2-21606-img0003.jpg)
![Western Blot: CHCHD3 Antibody (7G4) [NBP2-46281] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD3.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-7G4-Western-Blot-NBP2-46281-img0004.jpg "Western Blot: CHCHD3 Antibody (7G4) [NBP2-46281] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD3.")
![Immunohistochemistry: CHCHD3 Antibody (7G4) [NBP2-46281] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-7G4-Immunohistochemistry-NBP2-46281-img0003.jpg)
![Western Blot: CHCHD3 Antibody (7G4) [NBP2-46281] - Analysis of A549 cell lysate (35ug).](http://images.novusbio.com/fullsize/CHCHD3-Antibody-7G4-Western-Blot-NBP2-46281-img0005.jpg)
![Immunohistochemistry: CHCHD3 Antibody (7G4) [NBP2-46281] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-7G4-Immunohistochemistry-NBP2-46281-img0001.jpg)
![Immunohistochemistry: CHCHD3 Antibody (7G4) [NBP2-46281] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-7G4-Immunohistochemistry-NBP2-46281-img0002.jpg)
![Western Blot: CHCHD3 Antibody (OTI7G4) - Azide and BSA Free [NBP2-71975] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD3.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-OTI7G4-Azide-and-BSA-Free-Western-Blot-NBP2-71975-img0002.jpg "Western Blot: CHCHD3 Antibody (OTI7G4) - Azide and BSA Free [NBP2-71975] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD3.")
![Immunohistochemistry: CHCHD3 Antibody (OTI7G4) - Azide and BSA Free [NBP2-71975] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-OTI7G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71975-img0004.jpg)
![Western Blot: CHCHD3 Antibody (OTI7G4) - Azide and BSA Free [NBP2-71975] - Analysis of A549 cell lysate (35ug).](http://images.novusbio.com/fullsize/CHCHD3-Antibody-OTI7G4-Azide-and-BSA-Free-Western-Blot-NBP2-71975-img0001.jpg)
![Immunohistochemistry: CHCHD3 Antibody (OTI7G4) - Azide and BSA Free [NBP2-71975] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-OTI7G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71975-img0003.jpg)
![Immunohistochemistry: CHCHD3 Antibody (OTI7G4) - Azide and BSA Free [NBP2-71975] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-OTI7G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71975-img0005.jpg)
![Western Blot: CHCHD3 Antibody [NBP1-52008] - Analysis of CHCHD3 in Human Heart lysate (35 ug protein in RIPA buffer) using NBP1-52008 at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP1-52008-img0002.jpg "Western Blot: CHCHD3 Antibody [NBP1-52008] - Analysis of CHCHD3 in Human Heart lysate (35 ug protein in RIPA buffer) using NBP1-52008 at 1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-52008] - Staining of paraffin embedded Human Skeletal Muscle. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP1-52008-img0004.jpg)
![Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-52008] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP1-52008-img0003.jpg)
![Western Blot: CHCHD3 Antibody [NBP1-52009] - Staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP1-52009-img0003.jpg "Western Blot: CHCHD3 Antibody [NBP1-52009] - Staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-52009] - Staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Immunohistochemistry-Paraffin-NBP1-52009-img0001.jpg)
![Immunoprecipitation: CHCHD3 Antibody [NBP3-38566] - Immunoprecipitation analysis of 600 ug extracts of Mouse liver cells using 3 ug CHCHD3 antibody. Western blot was performed from the immunoprecipitate using CHCHD3 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38566_rabbit-chchd3-pab-10122024120684.jpg "Immunoprecipitation: CHCHD3 Antibody [NBP3-38566] - Immunoprecipitation analysis of 600 ug extracts of Mouse liver cells using 3 ug CHCHD3 antibody. Western blot was performed from the immunoprecipitate using CHCHD3 antibody at a dilution of 1:1000.")
![Immunocytochemistry/ Immunofluorescence: CHCHD3 Antibody [NBP3-38566] - Confocal immunofluorescence analysis of U2OS cells using CHCHD3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38566_rabbit-chchd3-pab-101220241214413.jpg)
![Western Blot: CHCHD3 Antibody [NBP3-38566] - Western blot analysis of various lysates using CHCHD3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 40s.](http://images.novusbio.com/fullsize/nbp3-38566_rabbit-chchd3-pab-1012202411592842.jpg)
![Western Blot: CHCHD3 Antibody [NBP1-56871] - Titration: 2 ug/ml Positive Control: whole cell protein extracts, immortalized human fibroblasts.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP1-56871-img0002.jpg "Western Blot: CHCHD3 Antibody [NBP1-56871] - Titration: 2 ug/ml Positive Control: whole cell protein extracts, immortalized human fibroblasts.")
![Western Blot: CHCHD3 Antibody [NBP1-56871] - This Anti-CHCHD3 antibody was used in Western Blot of Placenta tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP1-56871-img0001.jpg)
![Immunoprecipitation:CHCHD3 AntibodyNBP3-29683] -Detection of human CHCHD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHCHD3 antibody NBP3-29683 (lot NBP3-29683-1) used for IP at 6 µg per reaction. CHCHD3 was also immunoprecipitated by rabbit anti-CHCHD3 antibody BL21538. For blotting immunoprecipitated CHCHD3, NBP3-29683 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29683_rabbit-chchd3-pab-3012202412392159.jpg "Immunoprecipitation:CHCHD3 AntibodyNBP3-29683] -Detection of human CHCHD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHCHD3 antibody NBP3-29683 (lot NBP3-29683-1) used for IP at 6 µg per reaction. CHCHD3 was also immunoprecipitated by rabbit anti-CHCHD3 antibody BL21538. For blotting immunoprecipitated CHCHD3, NBP3-29683 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: CHCHD3 Antibody [NBP1-56870] - Hela cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CHCHD3-Antibody-Western-Blot-NBP1-56870-img0002.jpg "Western Blot: CHCHD3 Antibody [NBP1-56870] - Hela cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CHCHD3 Overexpression Lysate (Adult Normal) [NBL1-09142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHCHD3.](http://images.novusbio.com/fullsize/CHCHD3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09142-img0002.jpg "Western Blot: CHCHD3 Overexpression Lysate (Adult Normal) [NBL1-09142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHCHD3.")
![SDS-Page: CHCHD3 Protein [NBP2-22791]](http://images.novusbio.com/fullsize/CHCHD3-Protein-SDS-Page-NBP2-22791-img0001.jpg "SDS-Page: CHCHD3 Protein [NBP2-22791]")