Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - Localization of PIEZO1 on MLO-Y4 primary cilia and plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 um. Image ...read more
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Western Blot: PIEZO1 Antibody [NBP1-78537] - Analysis of MCF7 cell lysate using PIEZ01 antibody (NBP1-78537) at 2 ug/mL.
Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.
Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Histological analysis and the expression of PIEZO1 (NBP1-78537) and collage II (NBP1-77795) in human nucleus pulposus tissues. A, B Hematoxylin and eosin (H&E) ...read more
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - Green: 2 hours at RT with CLTC antibody diluted 1:100 in PBS-Triton 0.2%X-100/BSA 1%. Red: Phalloidin Blue: DAPI. Image from verified customer ...read more
Simple Western: PIEZO1 Antibody - BSA Free [NBP1-78537] - Piezo1 expression profiling, cellular mechanotransduction, and biomechanical correlation analysis across brain regions of different donors. The relative ...read more
Immunohistochemistry: PIEZO1 Antibody - BSA Free [NBP1-78537] - Piezo1 expression & cell function of human nucleus pulposus cells under mechanical stress. A NP cells received various mechanical stress treatment. Cell ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for PIEZO1 Antibody - BSA Free
FAM38A
KIAA0233
member A
membrane protein induced by beta-amyloid treatment
Mib
PIEZO1
piezo-type mechanosensitive ion channel component 1
Protein FAM38A
Background
Piezo1 (Mib, FAM38A) is a mechanosensitive ion channel which responds to mechanically activated currents in numerous cells, including the brain, bladder, colon, kidney, lung, and skin. However, mRNA expression of Piezo1 is weak in the DRG, unlike Piezo2. The expression of Piezo1 is induced in Alzheimer's associated senile plaques (PMID 16854388), as well as integrin activation through R-Ras signaling (PMID 20016066). R-Ras is recruited to the endoplasmic reticulum which increases calcium release, leading to the activation of the protease calpain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.
We have publications tested in 12 applications: Block/Neutralize, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, In vitro, In vivo assay, KO, Simple Western, WB.
Lee W, Nims RJ, Savadipour A et al. Osteoarthritis: articular chondrocyte inflammatory signaling leads to enhanced gene expression and function of mechanotransduction channel Piezo1 as a pathogenic feed-forward mechanism bioRxiv 2020-01-01 (KO, ICC/IF, Porcine, Human)
Luo H, Liu H, Bian W et al. Yoda1 Activates Piezo1 in Vitro to Simulate the Upregulation of Piezo1 in the Infected Brain: Piezo1 Participates in the Immune Activation of Microglia. Research Square 2021-03-11 (In vitro, Human)
Li X, Liu Q, Zhang H Effects of mechanosensitive ion channel Piezo1 on Osteoarthritic articular chondrocytes Chronic Diseases Prevention Review (ICC/IF, Human)
FAQs for PIEZO1 Antibody (NBP1-78537). (Showing 1 - 1 of 1 FAQs).
What is the expected molecular weight of the detected band in WB (mouse protein) in NBP1-78537? Has there been detection at lower molecular weight?
This human protein has at least one isoform with the major one has 2,521 a.a. with the calculated size of 286,790 Da. If considering the post-translational modifications (phosphorylated a.a. and glycosylations), the apparent size could be considerable larger (https://www.uniprot.org/uniprot/Q92508#sequences). Above website also shows some potential isoforms with the much smaller sizes (in 300 a.a or less). Whereas the counterpart in mouse has the major isoform of 2,547 a.a with 292,002 Da, and 4 potential isoforms (ranging from 1000 a.a. - 2500+ a.a.; https://www.uniprot.org/uniprot/E2JF22#sequences) [however it doesn't mean that there is no other smaller ones like human; just that probably no scientist has looked for them, because the proteins in the high mammals are very conserved]. 1) In terms of detecting the smaller PIEZO1 species, the WB image (fig# 1) done by our manufacturing lab on the MCF7 cell lysate shows that many smaller bands below the expected PIEZO1 band (above 251 kDa). 2) I think they could not assign any of the smaller bands to the protein target was because a, for expressing the large proteins in vivo and in vitro, they intend to be pre-mature terminated of the chain synthesis. Therefore the shorter chain(s) can vary in size(s) in the different cases. 3) I checked online and found that https://www.frontiersin.org/articles/10.3389/fnagi.2018.00332/full shows rat PIEZO1 has many smaller bands (Fig 1); seems that it was a wide-spread observation.4) All rabbit polyclonal antibodies, more or less, have some backgrounds/non-specific bands, because of the nature of the host. Only performing the steps to block the membranes well and incubate the Abs at room temp for 1 hr (or less) [and use less Ab as possible] to reduce the backgrounds/non-specific bands.
Touch Infographic: From Touch Receptors to the Brain The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for ... Read full blog post.
PIEZO1: A Mechanosensitive Ion Channel Protein PIEZO1, and its close homologue PIEZO2, are mechanosensitive ion channel proteins. Mechanosensitive ion channels couple protein conformation to the mechanics of the surrounding membrane, and switch between a closed state and an open state in response ... Read full blog post.
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![Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - Localization of PIEZO1 on MLO-Y4 primary cilia and plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//www.ciliajournal.com/content/4/1/7) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PIEZO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78537-img0009.jpg "Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - Localization of PIEZO1 on MLO-Y4 primary cilia and plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//www.ciliajournal.com/content/4/1/7) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-78537 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/PIEZO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78537-img0008.jpg "Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-78537 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: PIEZO1 Antibody [NBP1-78537] - Analysis of MCF7 cell lysate using PIEZ01 antibody (NBP1-78537) at 2 ug/mL.](http://images.novusbio.com/fullsize/PIEZO1-Antibody-Western-Blot-NBP1-78537-img0007.jpg "Western Blot: PIEZO1 Antibody [NBP1-78537] - Analysis of MCF7 cell lysate using PIEZ01 antibody (NBP1-78537) at 2 ug/mL.")
![Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PIEZO1-Antibody-Immunohistochemistry-NBP1-78537-img0006.jpg "Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.")
![Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Histological analysis and the expression of PIEZO1 (NBP1-78537) and collage II (NBP1-77795) in human nucleus pulposus tissues. A, B Hematoxylin and eosin (H&E) staining in human NP tissues of the control or the IVDD group (200×). C, D PIEZO1 immunostaining in human NP tissues of the control or the IVDD group (100×). E The percentage of PIEZO1 positive cells to all cells in the field (%). F, G Collagen II immunostaining in human NP tissues of the control or the IVDD group (100×). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35606793/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-78537_rabbit-polyclonal-piezo1-antibody-2552023153954.jpg "Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Histological analysis and the expression of PIEZO1 (NBP1-78537) and collage II (NBP1-77795) in human nucleus pulposus tissues. A, B Hematoxylin and eosin (H&E) staining in human NP tissues of the control or the IVDD group (200×). C, D PIEZO1 immunostaining in human NP tissues of the control or the IVDD group (100×). E The percentage of PIEZO1 positive cells to all cells in the field (%). F, G Collagen II immunostaining in human NP tissues of the control or the IVDD group (100×). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35606793/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - Green: 2 hours at RT with CLTC antibody diluted 1:100 in PBS-Triton 0.2%X-100/BSA 1%. Red: Phalloidin Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-78537_rabbit-polyclonal-piezo1-antibody-immunocytochemistry-immunofluorescence-162023153059..jpg "Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - Green: 2 hours at RT with CLTC antibody diluted 1:100 in PBS-Triton 0.2%X-100/BSA 1%. Red: Phalloidin Blue: DAPI. Image from verified customer review.")
![Simple Western: PIEZO1 Antibody - BSA Free [NBP1-78537] - Piezo1 expression profiling, cellular mechanotransduction, and biomechanical correlation analysis across brain regions of different donors. The relative abundance of (A) Piezo1, (B) YAP, (C) pYAP, and (D) beta-catenin in the regions of WM (magenta), GW junction (purple), and pons (green) for individual donors (D1-4). (E, top left) Spearman correlation between Piezo1 relative fluorescence units (RFU) and stiffness; GW Junction (orange) ns, WM (blue) r = -0.5341, p 250 kDa & >150 kDa. There was also a prominent immunopositive band at >75 kDa in the P1 & P14 lenses, the levels of which appeared to be decreased in the P16 lenses. (D). Piezo1 immunopositive bands of >250 & >75 kDa were present predominantly in the lens fiber samples (P21 & P27) compared to the lens epithelium (P21). (E,F). Immunofluorescence analysis of Piezo1 in the P1 mouse lens (the sagittal plane of the cryosection) revealed that the protein distributes predominantly to lens fibers relative to the epithelium (boxed area in panel (E) was magnified & shown in panel (F)). (G) Shows background immunofluorescence with secondary antibody alone. GAPDH: Loading control; Epi: Epithelium; Bars: Image magnification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35563101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78537_rabbit-polyclonal-piezo1-antibody-310202416175273.jpg "Simple Western: PIEZO1 Antibody - BSA Free [NBP1-78537] - Piezo1 expression profiling, cellular mechanotransduction, and biomechanical correlation analysis across brain regions of different donors. The relative abundance of (A) Piezo1, (B) YAP, (C) pYAP, and (D) beta-catenin in the regions of WM (magenta), GW junction (purple), and pons (green) for individual donors (D1-4). (E, top left) Spearman correlation between Piezo1 relative fluorescence units (RFU) and stiffness; GW Junction (orange) ns, WM (blue) r = -0.5341, p 250 kDa & >150 kDa. There was also a prominent immunopositive band at >75 kDa in the P1 & P14 lenses, the levels of which appeared to be decreased in the P16 lenses. (D). Piezo1 immunopositive bands of >250 & >75 kDa were present predominantly in the lens fiber samples (P21 & P27) compared to the lens epithelium (P21). (E,F). Immunofluorescence analysis of Piezo1 in the P1 mouse lens (the sagittal plane of the cryosection) revealed that the protein distributes predominantly to lens fibers relative to the epithelium (boxed area in panel (E) was magnified & shown in panel (F)). (G) Shows background immunofluorescence with secondary antibody alone. GAPDH: Loading control; Epi: Epithelium; Bars: Image magnification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35563101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry: PIEZO1 Antibody - BSA Free [NBP1-78537] - Piezo1 expression & cell function of human nucleus pulposus cells under mechanical stress. A NP cells received various mechanical stress treatment. Cell viability was measured by MTT assay. B Gene expression of Piezo1 was measured by real-time PCR. C, D Western blot analysis of Piezo1’s protein level. Total beta -actin served as loading controls. E, F Immunofluorescence staining analysis of Piezo1 expression. Relative fluorescent levels of Piezo1 were measured by Image J software. G Concentrations of pro-inflammatory cytokines TNF-alpha , IL-1 beta , & IL-6 in supernatants were measured by ELISA. H Mitochondrial membrane potential was measured by JC-1 probe & flow cytometer. I OCR of cells were measured by Seahorse XFe96 Extracellular Flux Analyzer at basal conditions & with serial administration of oligomycin, FCCP & rotenone. J–L Gene & protein expressions of P53 & P16. Total beta -actin served as loading controls. *p 250 kDa was detected only in P30 lens homogenates derived from the Piezo1-tdT mice, but not in the wild-type lens. The positive control (lung tissue lysate from the Piezo1-tdT mice) also showed a robust expression of Piezo1-tdT. Lanes 1 & 2 represent two different loads of the total protein (75 & 150 µg, respectively). (B) The Piezo1-tdT fusion protein was detected predominantly in fiber cell lysates compared to lens epithelial lysates. (C). Immunofluorescence analysis revealed the Piezo1-tdT fusion protein distributing to lens fibers with localization to both the short & long arms of the hexagonal lens fibers (Left & middle panels are with low & high magnification, respectively). The right panel shows a second antibody (Alexa Flour 488) background control fluorescence staining in the Piezo1-tdT mouse lens section. Bars: Image magnification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35563101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78537_rabbit-polyclonal-piezo1-antibody-310202416163794.jpg "Immunohistochemistry: PIEZO1 Antibody - BSA Free [NBP1-78537] - Piezo1 expression & cell function of human nucleus pulposus cells under mechanical stress. A NP cells received various mechanical stress treatment. Cell viability was measured by MTT assay. B Gene expression of Piezo1 was measured by real-time PCR. C, D Western blot analysis of Piezo1’s protein level. Total beta -actin served as loading controls. E, F Immunofluorescence staining analysis of Piezo1 expression. Relative fluorescent levels of Piezo1 were measured by Image J software. G Concentrations of pro-inflammatory cytokines TNF-alpha , IL-1 beta , & IL-6 in supernatants were measured by ELISA. H Mitochondrial membrane potential was measured by JC-1 probe & flow cytometer. I OCR of cells were measured by Seahorse XFe96 Extracellular Flux Analyzer at basal conditions & with serial administration of oligomycin, FCCP & rotenone. J–L Gene & protein expressions of P53 & P16. Total beta -actin served as loading controls. *p 250 kDa was detected only in P30 lens homogenates derived from the Piezo1-tdT mice, but not in the wild-type lens. The positive control (lung tissue lysate from the Piezo1-tdT mice) also showed a robust expression of Piezo1-tdT. Lanes 1 & 2 represent two different loads of the total protein (75 & 150 µg, respectively). (B) The Piezo1-tdT fusion protein was detected predominantly in fiber cell lysates compared to lens epithelial lysates. (C). Immunofluorescence analysis revealed the Piezo1-tdT fusion protein distributing to lens fibers with localization to both the short & long arms of the hexagonal lens fibers (Left & middle panels are with low & high magnification, respectively). The right panel shows a second antibody (Alexa Flour 488) background control fluorescence staining in the Piezo1-tdT mouse lens section. Bars: Image magnification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35563101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
