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CD68/SR-D1 Antibody (298813) [Unconjugated]

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CD68 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD68 Monoclonal Antibody (Catalog # MAB2040) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.
CD68 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD68 Monoclonal Antibody (Catalog # MAB2040) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the ...read more
CD68/SR-D1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD68/SR-D1 Monoclonal Antibody (Catalog # MAB2040) at 15 µg/mL for 1 hour at room temperature followed by ...read more
Orthogonal Strategies: CD68/SR-D1 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 560591) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, Dual ISH-IHC, mIF
Clone
298813
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

  

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2040-SP
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CD68/SR-D1 Antibody (298813) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
Asn22-Ile320
Accession # AAB25811
Specificity
Detects human CD68/SR‑D1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD68
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC 5 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Multiplex Immunofluorescence 20 ug/mL
Publications
Read Publications using
MAB2040 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD68/SR-D1 Antibody (298813) [Unconjugated]

  • CD_antigen: CD68
  • CD68 antigen
  • CD68 molecule
  • CD68
  • DKFZp686M18236
  • gp110
  • LAMP4
  • macrophage antigen CD68
  • Macrosialin
  • SCARD1
  • scavenger receptor class D, member 1
  • SRD1
  • SR-D1

Background

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD68/SR-D1 Antibody (MAB2040)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD68
Uniprot