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CD5 Antibody (53-7.3) [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IP, CyTOF-ready, Func
Clone
53-7.3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB115U-100UG
Alexa Fluor 405
FAB115V-025
Alexa Fluor 488
FAB115G-100UG
Alexa Fluor 532
FAB115X
Alexa Fluor 594
FAB115T-100UG
Alexa Fluor 647
FAB115R-100UG
Alexa Fluor 700
FAB115N-025
Alexa Fluor 750
FAB115S-100UG
Allophycocyanin
FAB115A-025
Allophycocyanin/Cy7
FAB115APCCY7
Biotin
BAM115
DyLight 350
FAB115D
DyLight 405
FAB115E
DyLight 488
FAB115K
DyLight 550
FAB115L
DyLight 594
FAB115M
DyLight 650
FAB115W
DyLight 680
FAB115Y
DyLight 755
FAB115Z
Fluorescein
FAB115F
HRP
FAB115H
Janelia Fluor 525
FAB115JF525
Janelia Fluor 549
FAB115I
Janelia Fluor 646
FAB115J
Janelia Fluor 669
FAB115JF669
PE/Atto594
FAB115PEATT594
PE/Cy5.5
FAB115PECY55
PE/Cy7
FAB115PECY7
PerCP
FAB115C
Phycoerythrin
FAB115P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB115F
Carrier Free
FAB115P
Carrier Free
MAB115-SP
Carrier Free
FAB115N-025
Carrier Free
FAB115V-025
Carrier Free
FAB115A-025
Carrier Free
FAB115U-100UG
Carrier Free
FAB115T-100UG
Carrier Free
FAB115S-100UG
Carrier Free
FAB115R-100UG
Carrier Free
FAB115G-100UG
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CD5 Antibody (53-7.3) [Unconjugated] Summary

Immunogen
Mouse thymus or spleen
Specificity
Detects mouse CD5.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
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Applications/Dilutions

Dilutions
  • Blocking B Cell Proliferation
  • Complement-dependent Cytotoxicity
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD5 Antibody (53-7.3) [Unconjugated]

  • CD5 antigen (p56-62)
  • CD5 antigen
  • CD5 molecule
  • CD5
  • LEU1T-cell surface glycoprotein CD5
  • Lymphocyte antigen T1/Leu-1
  • T1

Background

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.

  1. Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
  4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
  5. Luo, W. et al. (1992) J. Immunol. 148:1630.
  6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD5