Recombinant Human CD3 zeta His Protein Summary
Description |
A recombinant protein with a C-terminal His-tag and corresponding to the amino acids 52-164 of Human CD3. Source: Baculovirus Amino Acid Sequence: ADPRVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPRHHHH HH |
Source |
Baculovirus |
Protein/Peptide Type |
Recombinant Protein |
Gene |
CD247 |
Purity |
>90%, by SDS-PAGE |
Endotoxin Note |
< 1.0 EU per 1ug of protein (determined by LAL method) |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
14.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 6.8), 50% glycerol, 1 mM DTT, 1 mM EDTA, 0.1 M NaCl |
Preservative |
No Preservative |
Concentration |
0.2 mg/ml |
Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant Human CD3 zeta His Protein
Background
T-cell surface glycoprotein CD3 zeta chain, also known as CD247, belongs to the CD3Z/FCER1G family. CD247 is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Recombinant human CD247 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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