Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-simple-western-3032023112047.jpg "Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Zrecombinant monoclonal antibody [BL-336-1B2] (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Flow-Cytometry-NBP2-76399-img0007.jpg)
![Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinantmonoclonal antibody [BL-336-1B2] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-NBP2-76399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2].Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-Paraffin-NBP2-76399-img0009.jpg)
![Immunoprecipitation: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by WB of immunoprecipitates from Jurkat lysate.Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunoprecipitation-NBP2-76399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Rabbit antiCD247/CD3Z recombinant monoclonal antibody[BL-336-1B2]. (Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-immunohistochemistry-paraffin-732024101321..jpg)
![Western Blot: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Whole cell lysate (10 ug) from Jurkat, U2OS, SR, HEK293T, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-western-blot-732024101522..jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Analysis in human lymph node and pancreas tissues using NBP1-85750 antibody. Corresponding CD247 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-85750-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Analysis in human lymph node and pancreas tissues using NBP1-85750 antibody. Corresponding CD247 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD3 zeta Antibody [NBP1-85750] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-NBP1-85750-img0007.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Staining of human Pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-85750-img0014.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-85750-img0008.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Staining of human Cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-85750-img0012.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Staining of human Lymph node shows strong membranous positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-85750-img0013.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-85750-img0015.jpg)
![Western Blot: CD3 zeta Antibody [H00000919-D01P] - Analysis of CD247 expression in rat brain.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-H00000919-D01P-img0007.jpg "Western Blot: CD3 zeta Antibody [H00000919-D01P] - Analysis of CD247 expression in rat brain.")
![Western Blot: CD3 zeta Antibody [H00000919-D01P] - Analysis of CD247 expression in IMR-32.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-H00000919-D01P-img0003.jpg)
![Western Blot: CD3 zeta Antibody [H00000919-D01P] - Analysis of CD247 expression in Raw 264.7.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-H00000919-D01P-img0004.jpg)
![Western Blot: CD3 zeta Antibody [H00000919-D01P] - Analysis of CD247 expression in mouse kidney.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-H00000919-D01P-img0005.jpg)
![Western Blot: CD3 zeta Antibody [H00000919-D01P] - Analysis of CD247 expression in mouse brain.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-H00000919-D01P-img0006.jpg)
![Western Blot: CD3 zeta Antibody [H00000919-D01P] - Analysis of CD247 expression in transfected 293T cell line by CD247 polyclonal antibody.Lane 1: CD247 transfected lysate(18.70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-H00000919-D01P-img0008.jpg)
![Proximity Ligation Assay: CD3 zeta Antibody [H00000919-D01P] - Analysis of protein-protein interactions between CD247 and CD3E. HeLa cells were stained with anti-CD247 rabbit purified polyclonal 1:1200 and anti-CD3E mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Proximity-Ligation-Assay-H00000919-D01P-img0002.jpg)
![Western Blot: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Analysis of CD3 zeta (CD247) in lysate of murine splenocytes (reducing conditions) by the monoclonal antibody H146-968.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-H146-968-Azide-Free-Western-Blot-NBP1-44683-img0001.jpg "Western Blot: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Analysis of CD3 zeta (CD247) in lysate of murine splenocytes (reducing conditions) by the monoclonal antibody H146-968.")
![Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Analysis (intracellular staining) of CD3 zeta (CD247) in human peripheral blood using the monoclonal antibody H146-968 PE](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-H146-968-Azide-Free-Flow-Cytometry-NBP1-44683-img0003.jpg)
![Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Flow Cytometry: CD3 zeta Antibody (H146-968) [PE] [NBP1-44683PE] - A cell surface stain was performed on hPBMCs with CD3 Zeta antibody (H146-968) NBP1-44683 (top image) and a matched isotype control (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PE.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-H146-968-Azide-Free-Flow-Cytometry-NBP1-44683-img0002.jpg)
![Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Western-Blot-NBP1-42561-img0011.jpg "Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of K562 cells using anti-CD247 mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Immunocytochemistry-Immunofluorescence-NBP1-42561-img0009.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of human Thymus tissues using anti-CD247 mouse mAb](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Immunohistochemistry-Paraffin-NBP1-42561-img0010.jpg)
![Flow Cytometry: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of Jurkat cells using anti-CD247 mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Flow-Cytometry-NBP1-42561-img0012.jpg)
![Western Blot: CD3 zeta Antibody (1T5R7) [NBP3-15349] - Western blot analysis of extracts of Jurkat cells, using CD3 zeta Rabbit mAb (NBP3-15349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-1T5R7-Western-Blot-NBP3-15349-img0002.jpg "Western Blot: CD3 zeta Antibody (1T5R7) [NBP3-15349] - Western blot analysis of extracts of Jurkat cells, using CD3 zeta Rabbit mAb (NBP3-15349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (1T5R7) [NBP3-15349] - Immunohistochemistry of paraffin-embedded human colon using CD3 zeta Rabbit mAb (NBP3-15349) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-1T5R7-Immunohistochemistry-Paraffin-NBP3-15349-img0001.jpg)
![Flow Cytometry: CD3 zeta Antibody (1T5R7) [NBP3-15349] - Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 ug/ml, blue line) or CD3 zeta Rabbit mAb (NBP3-15349, 10 ug/ml orange line), followed by goat anti-Rabbit pAb FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line) .](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-1T5R7-Flow-Cytometry-NBP3-15349-img0003.jpg)
![Flow Cytometry: CD3 zeta Antibody (1T5R7) [NBP3-15349] - Flow cytometry: Raji cells were stained with Rabbit IgG isotype control (AC042, 10 ug/ml, blue line) or CD3 zeta Rabbit mAb (NBP3-15349, 2.5 ug/ml orange line), followed by goat anti-Rabbit pAb FITC (1:200 dilution) staining. Non-fluorescently stained Raji cells was used as blank control (red line) .](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-1T5R7-Flow-Cytometry-NBP3-15349-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Detection of phosphorylated CD3 zeta (EM-26; light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr72-Antibody-EM-26-Immunocytochemistry-Immunofluorescence-NBP2-62193-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Detection of phosphorylated CD3 zeta (EM-26; light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.")
![Western Blot: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr72-Antibody-EM-26-Western-Blot-NBP2-62193-img0001.jpg)
![Western Blot: CD3 zeta [p Tyr111] Antibody (EM-55) [NBP2-37709] - Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55).](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr111-Antibody-EM-55-Western-Blot-NBP2-37709-img0003.jpg "Western Blot: CD3 zeta [p Tyr111] Antibody (EM-55) [NBP2-37709] - Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55).")
![Western Blot: CD3 zeta [p tyr 111] Antibody (EM-55) [NBP2-37709] - Analysis in lysates of HEK293T/17 cells transiently transfected with expression vector harboring gene for mCD3zeta. Lane 1) Unstimulated cells, Lane 2) Pervanadate-stimulated cells](http://images.novusbio.com/fullsize/CD3-zeta-p-tyr-111-Antibody-EM-55-Western-Blot-NBP2-37709-img0001.jpg)
![Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr142-Antibody-EM-54-Western-Blot-NBP2-37708-img0001.jpg "Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5")
![Immunocytochemistry/Immunofluorescence: CD3 zeta Antibody (4D10A6) [NBP1-47337] - Immunofluorescence analysis of peripheral blood T cells using CD3 mouse mAb (A) and flow cytometric analysis of eripheral blood T cells using CD3 mouse mAb (B).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4D10A6-Immunocytochemistry-Immunofluorescence-NBP1-47337-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD3 zeta Antibody (4D10A6) [NBP1-47337] - Immunofluorescence analysis of peripheral blood T cells using CD3 mouse mAb (A) and flow cytometric analysis of eripheral blood T cells using CD3 mouse mAb (B).")
![Flow Cytometry: CD3 zeta Antibody (4D10A6) [NBP1-47337] - FCM analysis CD3 expressed in human peripheral blood cells. Image using the Unpurified format of this antibody.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4D10A6-Flow-Cytometry-NBP1-47337-img0004.jpg)
![Western Blot: CD3 zeta [p Tyr153] Antibody (EM-17) [NBP2-62192] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr153-Antibody-EM-17-Western-Blot-NBP2-62192-img0001.jpg "Western Blot: CD3 zeta [p Tyr153] Antibody (EM-17) [NBP2-62192] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.")
![Western Blot: CD3 zeta Antibody [NBP2-15796] - Analysis of CD247 expression in transfected 293T cell line by CD247 polyclonal antibody. A: Non-transfected lysate. B: CD247 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-NBP2-15796-img0001.jpg "Western Blot: CD3 zeta Antibody [NBP2-15796] - Analysis of CD247 expression in transfected 293T cell line by CD247 polyclonal antibody. A: Non-transfected lysate. B: CD247 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")