CD3 zeta Antibody (BL-336-1B2) Summary
Additional Information |
Recombinant Monoclonal Antibody. |
Immunogen |
Immunogen sequence between amino acids 150-164 (C-Term) |
Predicted Species |
Mouse (100%), Porcine (100%), Sheep (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
CD247 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.5 ul per 1 - 10^6 cells
- Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
- Immunohistochemistry 1:100 - 1:500
- Immunohistochemistry-Paraffin 1:100 - 1:500
- Immunoprecipitation 20 ul/mg lysate
- Simple Western
- Western Blot 1:1000
|
Application Notes |
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Packaging, Storage & Formulations
Storage |
Store at 2 - 8 C / 1 year from date of receipt |
Buffer |
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA |
Preservative |
0.09% Sodium Azide |
Purity |
Antigen Affinity-purified |
Notes
This Product sold under License from Bethyl Laboratories, Inc.
Alternate Names for CD3 zeta Antibody (BL-336-1B2)
Background
T-cell surface glycoprotein CD3 zeta chain is encoded by the gene CD247, also known as CD3Z or CD3 zeta. CD247/CD3 zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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