Reactivity | MuSpecies Glossary |
Applications | WB |
Clone | 158113 |
Clonality | Monoclonal |
Host | Rat |
Conjugate | Unconjugated |
Immunogen | E. coli-derived recombinant mouse CCL22/MDC Gly25-Ser92 Accession # O88430 |
Specificity | Detects mouse CCL22/MDC in direct ELISAs and Western blots. Shows 12% cross-reactivity with recombinant human (rh) CCL22 and recombinant mouse (rm) MIP-3 alpha , 25% cross-reactivity with rmCTACK, 6% cross-reactivity with rhBLC/BCA-1, and no cross-reactivity with rcrMIP-1 alpha , rhMIP-1 alpha , rmMIP-1 alpha , rcrMIP-1 beta , rmMIP-1 beta , rhMIP-1 beta
rhPARC, rcrRANTES, rhRANTES, rmRANTES, rhTARC, or rmTARC, |
Source | N/A |
Isotype | IgG2a |
Clonality | Monoclonal |
Host | Rat |
Gene | CCL22 |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
|
Publications |
|
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration. |
Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.
Publication using MAB439 | Applications | Species |
---|---|---|
Kearley J, Robinson DS, Lloyd CM CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling. J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6. 2008-07-30 [PMID: 18672278] (ELISA Development, Mouse) | ELISA Development | Mouse |
Secondary Antibodies |
Isotype Controls |
Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.