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CCL22/MDC Antibody (158113) [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
158113
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB439-SP
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CCL22/MDC Antibody (158113) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # O88430
Specificity
Detects mouse CCL22/MDC in direct ELISAs and Western blots. Shows 12% cross-reactivity with recombinant human (rh) CCL22 and recombinant mouse (rm) MIP-3 alpha , 25% cross-reactivity with rmCTACK, 6% cross-reactivity with rhBLC/BCA-1, and no cross-reactivity with rcrMIP-1 alpha , rhMIP-1 alpha , rmMIP-1 alpha , rcrMIP-1 beta , rmMIP-1 beta , rhMIP-1 beta rhPARC, rcrRANTES, rhRANTES, rmRANTES, rhTARC, or rmTARC,
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CCL22
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB439 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL22/MDC Antibody (158113) [Unconjugated]

  • A-152E5.1
  • ABCD-1
  • CC chemokine STCP-1
  • C-C motif chemokine 22
  • CCL22
  • chemokine (C-C motif) ligand 22
  • DC/B-CK
  • Macrophage-derived chemokine
  • MDC
  • MDCStimulated T-cell chemotactic protein 1
  • MGC34554
  • SCYA22MDC(1-69)
  • small inducible cytokine A22
  • small inducible cytokine subfamily A (Cys-Cys), member 22
  • Small-inducible cytokine A22
  • STCP-1
  • stimulated T cell chemotactic protein 1

Background

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

  1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
  2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
  3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL22/MDC Antibody (MAB439)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA Development.


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Bioinformatics

Gene Symbol CCL22
Uniprot