![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0011.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, RbSpecies Glossary |
| Applications | WB, IHC, IP (-), KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457). |
| Predicted Species | Rat (100%), Chicken (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CSNK2A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | By WB, this antibody recognizes a band at ~42 kDa, representing CKII alpha. Not recommended for immunoprecipitation. *NB 100-378 is recommended for IP. The investigator should determine the optimal working dilution for a specific application. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-buffered Saline, 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Casein Kinase 2 alpha Antibody (NB100-377)Find related products by research area.
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| Gene Symbol | CSNK2A1 |
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| Uniprot |
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![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0013.jpg "Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 or 0.1 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0005.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 or 0.1 ug/ml.")
![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0012.jpg "Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")