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![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 or 0.1 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0005.jpg)
![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0012.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Analysis of CSNK2A1 expression in transfected 293T cell line by CSNK2A1 monoclonal antibody (M01), clone 3D9.Lane 1: CSNK2A1 transfected lysate(45.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Western-Blot-H00001457-M01-img0014.jpg "Western Blot: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Analysis of CSNK2A1 expression in transfected 293T cell line by CSNK2A1 monoclonal antibody (M01), clone 3D9.Lane 1: CSNK2A1 transfected lysate(45.1 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Analysis of monoclonal antibody to CSNK2A1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Immunocytochemistry-Immunofluorescence-H00001457-M01-img0011.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - CSNK2A1 monoclonal antibody (M01), clone 3D9 Analysis of CSNK2A1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Western-Blot-H00001457-M01-img0013.jpg)
![Proximity Ligation Assay: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Analysis of protein-protein interactions between TP53 and CSNK2A1. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-CSNK2A1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Proximity-Ligation-Assay-H00001457-M01-img0009.jpg)
![Sandwich ELISA: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Detection limit for recombinant GST tagged CSNK2A1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Sandwich-ELISA-H00001457-M01-img0012.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-278202415295566.jpg "Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-378-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-NB100-378-img0010.jpg)
![Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of human CKII alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 (lot NB100-378-2) used for IP at 6 ug/mg lysate. CKII alpha was also immunoprecipitated by a previous lot (lot NB100-378-1) of this antibody. For blotting immunoprecipitated CKII alpha, NB100-378 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunoprecipitation-NB100-378-img0013.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0022.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0016.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (S05-7F8) [NBP3-19853] - Western blot detection of Casein Kinase 2 alpha in Hela (Lane 1), CHO-K1 (Lane 2), C6 (Lane 3), mouse Brain (Lane 4), Ramos (Lane 5) using NBP3-19853 (1:1000 diluted)](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-S05-7F8-Western-Blot-NBP3-19853-img0001.jpg "Western Blot: Casein Kinase 2 alpha Antibody (S05-7F8) [NBP3-19853] - Western blot detection of Casein Kinase 2 alpha in Hela (Lane 1), CHO-K1 (Lane 2), C6 (Lane 3), mouse Brain (Lane 4), Ramos (Lane 5) using NBP3-19853 (1:1000 diluted)")
![Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Western-Blot-NBP2-15909-img0002.jpg "Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CKII alpha Antibody [NBP2-15909] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15909-img0003.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NBP2-15909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (NBP2-15909) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15909_rabbit-polyclonal-casein-kinase-2-alpha-antibody-91020242014672.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (2P3M7) [NBP3-15858] - Western blot analysis of extracts of various cell lines, using Casein Kinase 2 alpha antibody (NBP3-15858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-2P3M7-Western-Blot-NBP3-15858-img0001.jpg "Western Blot: Casein Kinase 2 alpha Antibody (2P3M7) [NBP3-15858] - Western blot analysis of extracts of various cell lines, using Casein Kinase 2 alpha antibody (NBP3-15858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Casein Kinase 2 alpha Antibody (2B6) [NBP3-15160] - Positive WB detected in: NIH/3T3 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, U-87 whole cell lysate. All lanes: Casein Kinase 2 alpha antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 30 KDa. Observed band size: 45 KDa.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-2B6-Western-Blot-NBP3-15160-img0001.jpg "Western Blot: Casein Kinase 2 alpha Antibody (2B6) [NBP3-15160] - Positive WB detected in: NIH/3T3 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, U-87 whole cell lysate. All lanes: Casein Kinase 2 alpha antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 30 KDa. Observed band size: 45 KDa.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 2 alpha Antibody [NBP2-57124] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57124-img0001.jpg "Immunocytochemistry/Immunofluorescence: Casein Kinase 2 alpha Antibody [NBP2-57124] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NBP2-68778] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68778-img0001.jpg "Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NBP2-68778] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts and Leydig cells.")