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Calnexin Antibody

Images

 
Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).
Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97485] - Analysis of human spleen tissue stained with Calnexin, pAb at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, Dr, Gp, Ha, Pm, Rb, Sh, XpSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, MS
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Calnexin Antibody Summary

Immunogen
Synthetic peptide corresponding to the sequence near the C-terminus of canine calnexin.
Marker
Endoplasmic Reticulum Membrane Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CANX
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy 1:10-1:500
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:250
  • Western Blot 1:1000
Application Notes
Detects a band of ~90kDa by Western blot. Flow Cytometry was reported in scientific literature. Simple Western reported by an internal validation: tested in human multiple myeloma; antibody dilution of 1:250; separated by size; detects a band at 119 kDa
Publications
Read Publications using
NBP1-97485 in the following applications:

  • 1 publication
  • 4 publications
  • IP
    2 publications
  • WB
    6 publications

Reactivity Notes

Quail (100%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Calnexin Antibody

  • calnexin
  • CNX
  • IP90FLJ26570
  • Major histocompatibility complex class I antigen-binding protein p88
  • P90

Background

Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calnexin Antibody (NBP1-97485)(12)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Porcine, Primate.

We have publications tested in 4 applications: FLOW, ICC/IF, IP, WB.


Filter By Application
FLOW
(1)
ICC/IF
(4)
IP
(2)
WB
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Rat
(2)
Canine
(1)
Porcine
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-97485 Applications Species
GOmez-Puerta S, Ferrero R, Hochstoeger T et al. Live imaging of the co-translational recruitment of XBP1 mRNA to the ER and its processing by diffuse, non-polarized IRE1 alpha eLife 2022-06-22 [PMID: 35730412] (WB) WB
Santamaria M, Pardo-Saganta A, Alvarez-Asiain L et al. Nuclear alpha1-Antichymotrypsin Promotes Chromatin Condensation and Inhibits Proliferation of Human Hepatocellular Carcinoma Cells. Gastroenterology 2013-01-04 [PMID: 23295442] (WB, Human) WB Human
L Scorrano et al. Mitofusin 2 tethers endoplasmic reticulum to mitochondria. Nature 456, 605 . 2008-01-01 [PMID: 19052620] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
J Oberwinkler et al. Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic beta cells. Nat. Cell Biol. 10, 1421 . 2008-01-01 [PMID: 18978782] (WB, Human) WB Human
M Knepper et al. LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat. Am. J. Physiol. Renal Physiol. 295, F1799 . 2008-01-01 [PMID: 18922883] (WB, Rat) WB Rat
G Barton et al. The ectodomain of Toll-like recepto r9 is cleaved to generate a functional receptor. Nature 456, 658 . 2008-01-01 [PMID: 18820679] (WB, Mouse) WB Mouse
RS Hegde et al. Substrate-specific function of the translocon-associated protein complex during translocation across the ER membrane. J. Cell Biol. 160, 529 . 2003-01-01 [PMID: 12578908] (WB, Porcine, Canine) WB Porcine, Canine
LJ Parent et al. Specificity of plasma membrane targeting by the rous sarcoma virus gag protein. J. Virol. 77, 470 . 2003-01-01 [PMID: 12477852] (ICC/IF) ICC/IF
K Nagata et al. Type XXVI collagen, a new member of the collagen family, is specifically expressed in the testis and ovary. J. Biol. Chem. 277, 37678 . 2002-01-01 [PMID: 12145293] (ICC/IF, Primate) ICC/IF Primate
M Edidin et al. Probing for membrane domains in the endoplasmic reticulum: retention and degradation of unassembled MHC class I molecules. Mol. Biol. Cell 13, 1566 . 2002-01-01 [PMID: 12006653] (IP, Mouse) IP Mouse
Show All 12 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calnexin Antibody (NBP1-97485) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Calnexin Antibody (NBP1-97485)

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Blogs on Calnexin.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

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Bioinformatics

Gene Symbol CANX
Entrez
Uniprot