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Calnexin Antibody

Images

 
Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.
Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97476] - Analysis of human placenta tissue stained with Calnexin, pAb at 5ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Gp, Ha, Pm, Rb, Sh, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IP, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Calnexin Antibody Summary

Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of canine calnexin.
Marker
Endoplasmic Reticulum Membrane Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CANX
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Single Cell Western 1:10
  • Western Blot 1:1000
Application Notes
Single Cell Western reported by an internal validation on U373 MG cells at a 1:10 dilution
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-97476 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Calnexin Antibody

  • calnexin
  • CNX
  • IP90FLJ26570
  • Major histocompatibility complex class I antigen-binding protein p88
  • P90

Background

Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calnexin Antibody (NBP1-97476)(3)

We have publications tested in 2 confirmed species: Human, C. elegans.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
All Applications
Filter By Species
Human
(1)
C. elegans
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-97476 Applications Species
Butler V, Cortopassi W, Argouarch A et al. C. elegans granulins promote an age-associated decline in protein homeostasis via lysosomal protease inhibition. CST. 2018-11-12 (WB) WB
Butler VJ, Gao F, Corrales CI et al. Age- and stress-associated C. elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response PLoS Genet. 2019-08-01 [PMID: 31398187] (WB, C. elegans) WB C. elegans
Park SJ, Jeong J, Park YU et al. Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics Sci Rep 2015-03-03 [PMID: 25732993] (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calnexin Antibody (NBP1-97476) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Calnexin Antibody (NBP1-97476)

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Blogs on Calnexin.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

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Bioinformatics

Gene Symbol CANX