Caldesmon/CALD1 Antibody (CALD1/1424R) - Azide and BSA Free

Recombinant Monoclonal Antibody.

Recombinant human full-length Caldesmon/CALD1 protein (Uniprot: Q05682 )

Cytoplasmic

Smooth Muscle Marker

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This monoclonal antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

IgG

Monoclonal

Rabbit

CALD1

Protein A or G purified

Test in a species/application not listed above to receive a full credit towards a future purchase.

Immunohistology (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.