![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024941471.jpg "Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL2379 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human Calbindin D-28K. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CALB1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Calbindin D-28K Antibody (NBP3-25392)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CALB1 |
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415939.jpg "Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024942549.jpg "Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249393563.jpg "Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415961.jpg "Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249371590.jpg "Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249355523.jpg "Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")