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Calbindin D/S100G Antibody [Unconjugated]

 (8 Publications)
 
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Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by ...read more
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Goat ...read more
Calbindin D was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3320-SP
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Calbindin D/S100G Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Calbindin D
Ala2-Asn261
Accession # P05937
Specificity
Detects human Calbindin D in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100G
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 5 ug/mL
Publications
Read Publications using
AF3320 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calbindin D/S100G Antibody [Unconjugated]

  • CABP
  • CABP;CABP1;CABP9K;CALB3;Protein S100-G
  • CABP1
  • CABP28K
  • CABP9K
  • Calbindin 3
  • Calbindin D
  • Calbindin-D
  • Calbindin-D9K
  • S100G

Background

Calbindin D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin D is widely expressed and regulates calcium homeostasis. Calbindin D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. Human, mouse, and rat Calbindin D share 98% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calbindin D/S100G Antibody (AF3320)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA Development (Detection), IHC, IHC-P.


Filter By Application
ELISA Development (Detection)
(1)
IHC
(3)
IHC-P
(2)
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Human
(1)
Mouse
(5)
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Showing Publications 1 - 8 of 8.
Publications using AF3320 Applications Species
Viventi S, Frausin S, Howden SE et al. In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia Stem cells translational medicine 3/18/2021 [PMID: 33734599]
Ashley J. Swain, Adriana Galvan, Thomas Wichmann, Yoland Smith Structural plasticity of GABAergic and glutamatergic networks in the motor thalamus of parkinsonian monkeys Journal of Comparative Neurology 2020-06-01 [PMID: 31808567]
M Zhang, Y Wang, J Geng, S Zhou, B Xiao Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice Cell Rep, 2019-02-05;26(6):1419-1431.e4. 2019-02-05 [PMID: 30726728] (IHC, Mouse) IHC Mouse
Allison M Bradbury Cerebrospinal fluid calbindin D concentration as a biomarker of cerebellar disease progression in Niemann-Pick type C1 disease J Pharmacol Exp Ther, 2016-06-15;0(0):. 2016-06-15 [PMID: 27307499] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Bifsha P, Yang J, Fisher R, Drouin J Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra. PLoS Genet, 2014-12-11;10(12):e1004863. 2014-12-11 [PMID: 25501001] (IHC-P, Mouse) IHC-P Mouse
Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2014-11-24;18(1):145-53. 2014-11-24 [PMID: 25420068] (IHC, Mouse) IHC Mouse
Di Giovannantonio , Luca G, Di Salvio , Michela, Omodei , Daniela, Prakash , Nilima, Wurst , Wolfgang, Pierani , Alessand, Acampora , Dario, Simeone , Antonio Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development, 2013-12-11;141(2):377-88. 2013-12-11 [PMID: 24335253] (IHC, Mouse) IHC Mouse
Di Salvio M, Di Giovannantonio LG, Acampora D Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat. Neurosci., 2010-11-07;13(12):1481-8. 2010-11-07 [PMID: 21057506] (IHC-P, Mouse) IHC-P Mouse

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Secondary Antibodies

 

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Check out the latest blog posts on Calbindin D/S100G.
Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.
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Bioinformatics

Gene Symbol S100G
Uniprot