![Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249425488.jpg "Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1787 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human Calbindin 1/2 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CALB1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CALB1 |
![Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249362752.jpg "Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249432860.jpg "Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Immunohistochemistry-Paraffin: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-212024939116.jpg "Immunohistochemistry-Paraffin: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249342761.jpg "Immunocytochemistry/Immunofluorescence: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Frozen: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin + Calretinin antibody [HL1787] detects Calbindin + Calretinin protein at cytoplasm by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: Calbindin + Calretinin stained by Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249432881.jpg "Immunohistochemistry-Frozen: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin + Calretinin antibody [HL1787] detects Calbindin + Calretinin protein at cytoplasm by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: Calbindin + Calretinin stained by Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:100. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249342739.jpg "Immunohistochemistry-Paraffin: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-212024940898.jpg "Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot Calbindin D/S100G Antibody [Unconjugated]](https://images.novusbio.com/images2/Calbindin_D_AF3320_Western_Blot_17076.jpg)
![Simple Western Calbindin D/S100G Antibody [Unconjugated]](https://images.novusbio.com/images2/Calbindin_D_AF3320_Immunohistochemistry_17486.jpg)
![Immunohistochemistry Calbindin D/S100G Antibody [Unconjugated]](https://images.novusbio.com/images2/af3320_human-calbindin-d-affinity-purified-polyclonal-ab-immunohistochemistry-1612202012853.jpg)
![Immunohistochemistry GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Immunohistochemistry_6730.jpg)
![Western Blot GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Western_Blot_19950.jpg)
![Simple Western GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Simple_Western_20168.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")