C1qR1/CD93 Antibody (R139) - BSA Free Summary
Immunogen |
The immunogen for this antibody was CD93. |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
CD93 |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:100-1:2000
- Flow Cytometry 1:10-1:1000
- Immunoprecipitation 1:10-1:500
- Western Blot 1:100-1:2000
|
Application Notes |
This R139 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. |
Reactivity Notes
This antibody is reactive to Human.
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
PBS (pH 7.2) |
Preservative |
0.09% Sodium Azide |
Concentration |
0.5 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for C1qR1/CD93 Antibody (R139) - BSA Free
Background
The monoclonal antibody R139 recognizes human CD93, also known as C1qRp. The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A. CD93 is predicted to play a role in the clearance of apoptotic cells. Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells. Expression on DCs is downregulated upon maturation. Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors. The monoclonal antibody R139 blocks C1q-mediated enhancement of phagocytosis. CD93 can be shed from the cell surface. This phenomenon can be measured using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA. The epitope for R139 resides in the EGF domains.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Secondary Antibodies
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