c-Myc Antibody

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, S. pombe.

We have publications tested in 7 applications: Chemotaxis, ICC/IF, IP, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, WB, Western Blot.

Showing Publications 1 - 10 of 28. Show All 28 Publications. Collapse Publications.

Publications using NB600-336	Applications	Species
Wells JR, Padua MB, Haaning AM et al. Non-coding cause of congenital heart defects: Abnormal RNA splicing with multiple isoforms as a mechanism for heterotaxy Human Genetics and Genomics Advances 2024-09-12 [PMID: 39275801]
Park ZM, Sporer AJ, Kraft K et al. Kar4, the yeast homolog of METTL14, is required for mRNA m6A methylation and meiosis PLOS Genetics 2023-08-21 [PMID: 37603553] (Western Blot)	Western Blot
Park ZM, Belnap E, Remillard M, Rose MD Vir1p, the Yeast Homolog of Virilizer, is Required for mRNA m 6 A Methylation and Meiosis bioRxiv : the preprint server for biology 2023-02-07 [PMID: 36798303] (Western Blot)	Western Blot
Park ZM, Sporer A, Kraft K et al. Kar4, the Yeast Homolog of METTL14, is Required for mRNA m (6) A Methylation and Meiosis bioRxiv 2023-08-16 [PMID: 36747717] (Western Blot)	Western Blot
Park Z, Sporer A, Kraft K et al. Kar4, the Yeast Homolog of METTL14, is Required for mRNA m6A Methylation and Meiosis bioRxiv 2023-01-30
Liang JH, Akhanov V, Ho A et al. Dopamine signaling from ganglion cells directs layer-specific angiogenesis in the retina Current biology : CB 2023-08-07 [PMID: 37572663]
Filidou E, Kandilogiannakis L, Tarapatzi G et al. A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood Biomedicines 2023-03-05 [PMID: 36979766] (Immunocytochemistry/ Immunofluorescence, Human)	Immunocytochemistry/ Immunofluorescence	Human
Xiong Y, Wang L, Xu S et al. Small molecule Z363 co-regulates TAF10 and MYC via the E3 ligase TRIP12 to suppress tumour growth Clinical and Translational Medicine 2023-01-13 [PMID: 36639831] (Immunoprecipitation, Western Blot)	Immunoprecipitation, Western Blot
Alzaydi MM, Abdul-Salam VB, Whitwell HJ et al. Intracellular Chloride Channels Regulate Endothelial Metabolic Reprogramming in Pulmonary Arterial Hypertension American journal of respiratory cell and molecular biology 2022-10-20 [PMID: 36264759]
Zeb A, Choubey V, Gupta R Et al. A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagy Redox biology 2021-11-11 [PMID: 34801863]
Akella NM, Le Minh G, Ciraku L et al. O-GlcNAc Transferase Regulates Cancer Stem-like Potential of Breast Cancer Cells Mol. Cancer Res. 2020-01-23 [PMID: 31974291] (WB, Human)	WB	Human
Martinez-Velez N, Garcia-Moure M, Marigil M et al. Blood feeding activates the vitellogenic stage of oogenesis in the mosquito Aedes aegypti through inhibition of glycogen synthase kinase 3 by the insulin and TOR pathways Dev Biol 2019-06-03 [PMID: 31153832]
Yang L, Liu Y, Wang M et al. Quercetin-induced apoptosis of HT-29 colon cancer cells via inhibition of the Akt-CSN6-Myc signaling axis. Mol Med Rep 2016-11-01 [PMID: 27748879] (Human)		Human
Clairfeuille T, Norwood SJ, Qi X et al. Structure and membrane binding properties of the endosomal tetratricopeptide repeat (TPR) domain-containing sorting nexins SNX20 and SNX21 J. Biol. Chem. 2015-04-16 [PMID: 25882846]
Park ZM It Takes Two to Tetrad: Dissecting the Role of Kar4 and Vir1 in Meiosis and mRNA m6 A Methylation Thesis 2023-01-01 (WB)	WB
Naffin-Olivos JL, Georgieva M, Goldfarb N et al. Mycobacterium tuberculosis Hip1 Modulates Macrophage Responses through Proteolysis of GroEL2. PLoS Pathog. 2014-05-01 [PMID: 24830429] (WB)	WB
Lin YN, Wu CT, Lin YC et al. CEP120 interacts with CPAP and positively regulates centriole elongation. J Cell Biol 2013-07-22 [PMID: 23857771] (ICC/IF)	ICC/IF
Lin YC, Chang CW, Hsu WB et al. Human microcephaly protein CEP135 binds to hSAS-6 and CPAP, and is required for centriole assembly EMBO J 2013-03-19 [PMID: 23511974] (IP, Human)	IP	Human
Tomalka AG, Stopford CM, Lee PC, Rietsch A. A translocator-specific export signal establishes the translocator-effector secretion hierarchy that is important for type III secretion system function Mol Microbiol 2012-12-01 [PMID: 23121689] (WB)	WB
Tanaka A, Tanizawa H, Sriswasdi S, et al. Epigenetic Regulation of Condensin-Mediated Genome Organization during the Cell Cycle and upon DNA Damage through Histone H3 Lysine 56 Acetylation Mol Cell 2012-10-17 [PMID: 23084836] (Chemotaxis)	Chemotaxis
Fontaine SN, Bauer SP, Lin X et al. Replacement of charged and polar residues in the coiled-coiled interface of huntingtin-interacting protein 1 (HIP1) causes aggregation and cell death FEBS Lett 2012-07-23 [PMID: 22835334] (WB, Human)	WB	Human
Park YK, Park H Differentiated embryo chondrocyte 1 (DEC1) represses PPAR gamma 2 gene through interacting with CCAAT/enhancer binding protein beta (C/EBPbeta) Mol Cells 2012-05-22 [PMID: 22610404] (ICC/IF, WB, Mouse)	ICC/IF, WB	Mouse
Tang C, Lin S, Hsu W et al. The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. EMBO J. 2011-01-01 [PMID: 22020124]
Zheng Q, Su H, Ranek MJ, Wang X. Autophagy and p62 in Cardiac Proteinopathy. Circ Res. 2011-06-09 [PMID: 21659648] (ICC/IF, Mouse)	ICC/IF	Mouse
Wei JR, Krishnamoorthy V, Murphy K et al. Depletion of antibiotic targets has widely varying effects on growth. Proc Natl Acad Sci U S A;108(10):4176-81. 2011-03-08 [PMID: 21368134]
Tang CJ, Fu RH, Wu KS et al. CPAP is a cell-cycle regulated protein that controls centriole length. Nat Cell Biol;11(7):825-31. 2009-07-01 [PMID: 19503075] (IP, WB, Human)	IP, WB	Human
Cam H P, Noma K et al. Host genome surveillance for retrotransposons by transposon-derived proteins. Nature 2008-01-24 [PMID: 18094683] (IP, S. pombe)	IP	S. pombe
Sun Y et al. Rab6 regulates both ZW10/RINT-1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis. Mol Biol Cell;18(10):4129-42. 2007-10-01 [PMID: 17699596]
Show All 28 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Human	06/04/2021	View Summary Application Western Blot Sample Tested HEK293 Species Human Lot B-10
Enlarge	5 reviewed by: Natalie Erdmann	IF	Human	10/02/2015	View Summary Application Immunofluorescence Sample Tested K-562, U266, Daudi, normal human T cells Species Human Lot B
Enlarge	5 reviewed by: Verified Customer	WB	Human	07/24/2014	View Summary Application Western Blot Sample Tested WB for c-Myc in MOLT-4 cells Species Human

Array NB600-336

• c-Myc Antibodies
• c-Myc ELISA Kits
• c-Myc Lysate
• c-Myc Proteins