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BRM Antibody

Images

 
Western Blot: BRM Antibody [NBP1-80042] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-80042] - Human Skin Tissue Squamous epithelial cells (Indicated with Arrows), 4-8ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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BRM Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptide directed towards the middle region of human SMARCA2. Peptide sequence VINYKDSSGRQLSEVFIQLPSRKELPEYYELIRKPVDFKKIKERIRNHKY. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCA2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for BRM Antibody

  • ATP-dependent helicase SMARCA2
  • BAF190B
  • BRG1-associated factor 190B
  • BRM
  • BRMFLJ36757
  • EC 3.6.1
  • EC 3.6.4.-
  • global transcription activator homologous sequence
  • hBRM
  • hBRMMGC74511
  • hSNF2a
  • probable global transcription activator SNF2L2
  • Protein brahma homolog
  • SMARCA2
  • SNF2
  • SNF2/SWI2-like protein 2
  • SNF2A
  • SNF2-alpha
  • SNF2L2
  • SNF2L2BAF190
  • SNF2LA
  • SNF2-like 2
  • Sth1p
  • subfamily a, member 2
  • sucrose nonfermenting 2-like protein 2
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 2
  • SWI2

Background

BRM is encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SMARCA2