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BMP-2/BMP-4 Antibody (100230) [Unconjugated]

 (15 Publications)
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Recombinant Human BMP‑2 (Catalog # 355-BM) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Human BMP‑2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-2/BMP-4 Monoclonal Antibody (Catalog # MAB3552). The ND50 is typically 5-15 µg/mL in the presence of heparin (2 µg/mL).
Recombinant Human BMP‑2 (Catalog # 355-BM) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clone
100230
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAM3552
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3552-SP
Carrier Free
MAB3552-500
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BMP-2/BMP-4 Antibody (100230) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP-2
Gln283-Arg396
Accession # P12643
Specificity
Detects human BMP-2 and human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant zebra fish (rzf) BMP-2 and rhBMP-4, 20%-40% cross-reactivity with rhBMP-3, -3b, -6, and no cross-reactivity with rhBMP-5, -7, -10, or -15 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BMP2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB3552 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-2/BMP-4 Antibody (100230) [Unconjugated]

  • BDA2
  • BMP-2
  • BMP-2/BMP-4
  • BMP2A
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2

Background

Bone Morphogenetic Protein 2 (BMP-2) and BMP-4 are two of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta ) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMP-2/BMP-4 Antibody (MAB3552)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 6 applications: Bioassay, Cell Culture, IHC, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Bioassay
(2)
Cell Culture
(1)
IHC
(1)
Immunoprecipitation
(1)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB3552 Applications Species
S Nagel, C Pommerenke, C Meyer, RAF MacLeod The Hematopoietic TALE-Code Shows Normal Activity of IRX1 in Myeloid Progenitors and Reveals Ectopic Expression of IRX3 and IRX5 in Acute Myeloid Leukemia International Journal of Molecular Sciences, 2022-03-16;23(6):. 2022-03-16 [PMID: 35328612] (Cell Culture, Human) Cell Culture Human
W Chen, SS Foo, E Hong, C Wu, WS Lee, SA Lee, D Evseenko, MEL Moreira, A García-Sas, G Cheng, K Nielsen-Sa, P Brasil, E Avvad-Port, JU Jung Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses Nature Microbiology, 2021-01-28;0(0):. 2021-01-28 [PMID: 33510473] (Bioassay, Human) Bioassay Human
Bersini S, Schulte R, Huang L et al. Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome eLife 9/8/2020 [PMID: 32896271]
Susanna Canali, Chia-Yu Wang, Kimberly B. Zumbrennen-Bullough, Abraham Bayer, Jodie L. Babitt Bone morphogenetic protein 2 controls iron homeostasis in mice independent of Bmp6 American Journal of Hematology 11/1/2017 [PMID: 28815688]
CY Wang, AB Core, S Canali, KB Zumbrennen, S Ozer, L Umans, A Zwijsen, JL Babitt Smad1/5 is required for erythropoietin-mediated suppression of hepcidin in mice Blood, 2017-04-24;0(0):. 4/24/2017 [PMID: 28438754]
Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis. PLoS ONE, 2015-06-25;10(6):e0131453. 2015-06-25 [PMID: 26110906] (Bioassay, Human) Bioassay Human
Bruce A. Boswell, Linda S. Musil Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells Molecular Biology of the Cell 7/1/2015 [PMID: 25947138]
Paul T. Hamilton, Michelle S. Jansen, Sathya Ganesan, R. Edward Benson, Robin Hyde-DeRuyscher, Wayne F. Beyer et al. Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides PLoS ONE 8/8/2013 [PMID: 23950987]
Rafael Kramann, Simone K. Couson, Sabine Neuss, Uta Kunter, Manfred Bovi, Jörg Bornemann et al. Exposure to Uremic Serum Induces a Procalcific Phenotype in Human Mesenchymal Stem Cells Arteriosclerosis, Thrombosis, and Vascular Biology 9/1/2011 [PMID: 21680902]
Sonia Stefanovic, Nesrine Abboud, Stéphanie Désilets, David Nury, Chad Cowan, Michel Pucéat Interplay of Oct4 with Sox2 and Sox17: a molecular switch from stem cell pluripotency to specifying a cardiac fate Journal of Cell Biology 9/7/2009 [PMID: 19736317]
Show All 15 Publications.

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Bioinformatics

Gene Symbol BMP2
Uniprot