![Western Blot: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Azide-and-BSA-Free-Western-Blot-NBP2-80599-img0002.jpg "Western Blot: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140. Image from the standard format of this antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IP, ChIP |
| Clone | LLBmi1-1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Human Bmi1 protein [Swiss-Prot# P35226] |
| Localization | Nucleus. Cytoplasm. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BMI1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This Bmi1 Antibody (LLBmi1-1) is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation and Western blot, where a band can be seen at approximately 42 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BMI-1 Antibody (NBP2-80599)Find related products by research area.
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| Gene Symbol | BMI1 |
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-BMI-1 Antibody (LLBmi1-1) NBP2-80599 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80599-img0007.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-BMI-1 Antibody (LLBmi1-1) NBP2-80599 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80599-img0005.jpg "Flow Cytometry: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Western Blot: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Azide-and-BSA-Free-Western-Blot-NBP2-80599-img0001.jpg "Western Blot: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-BMI-1 Antibody (LLBmi1-1) NBP2-80599 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80599-img0006.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-BMI-1 Antibody (LLBmi1-1) NBP2-80599 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80599-img0004.jpg "Flow Cytometry: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher). Image from the standard format of this antibody.")
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