![Western Blot: BCAS1 Antibody [NB100-681] - Western blot analysis of BCAS1 from 15 ug of MCF-7 cell lysate using NB100-681 at 1:1000 dilution. BCAS1 is a 60 kDa protein: however it forms homodimers, which are detected in MCF-7 cells.](http://images.novusbio.com/fullsize/BCAS1-Antibody-Western-Blot-NB100-681-img0005.jpg "Western Blot: BCAS1 Antibody [NB100-681] - Western blot analysis of BCAS1 from 15 ug of MCF-7 cell lysate using NB100-681 at 1:1000 dilution. BCAS1 is a 60 kDa protein: however it forms homodimers, which are detected in MCF-7 cells.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Reacts with residues 532-550 [DTNSLQNGDKLQKRPEKRQ] of human NABC1. |
| Specificity | Reacts with human NABC1. Reactivity to other species unknown. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BCAS1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody may be used for Western blot analysis where 2 bands are seen at ~120 and ~80 kDa. NABC1 is a 60 kDa protein; however it forms dimers, which are readily detectable in MCF-7 cells. No other applications have been tested. Suggested Working Dilutions:* Western blot - 1:500 to 1:1,000 *The investigator should determine the optimal dilution for a specific application. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: BCAS1 Antibody [NB100-681] - Detection of NABC1 in MCF-7 whole cell lysate (15 ug) using a 1:1,000 dilution of NB 100-681.](http://images.novusbio.com/fullsize/BCAS1-Antibody-Western-Blot-NB100-681-img0004.jpg "Western Blot: BCAS1 Antibody [NB100-681] - Detection of NABC1 in MCF-7 whole cell lysate (15 ug) using a 1:1,000 dilution of NB 100-681.")
![Simple Western: BCAS1 Antibody [NB100-681] - Simple Western lane view shows a specific band for BCAS1 in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/BCAS1-Antibody-Simple-Western-NB100-681-img0003.jpg "Simple Western: BCAS1 Antibody [NB100-681] - Simple Western lane view shows a specific band for BCAS1 in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Intracellular Staining by Flow Cytometry EMP3 Antibody (439949) [Unconjugated]](https://images.novusbio.com/images2/EMP3_MAB4505_Flow_Cytometry_8469.jpg)
![Intracellular Staining by Flow Cytometry EMP3 Antibody (439949) [Unconjugated]](https://images.novusbio.com/images/antibody/mab4505_human-emp3-mab-clone-439949-intracellular-staining-by-flow-cytometry-1782023142644.jpg)
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")