![Western Blot: B-Raf Antibody [NBP2-15531] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B Raf antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-15531-img0007.jpg "Western Blot: B-Raf Antibody [NBP2-15531] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B Raf antibody diluted by 1:1000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human B-Raf. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BRAF |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 84 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531] - Paraffin-embedded mouse testis. B-Raf antibody [N2C1], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunohistochemistry-Paraffin-NBP2-15531-img0008.jpg "Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531] - Paraffin-embedded mouse testis. B-Raf antibody [N2C1], Internal dilution: 1:500.")
![Western Blot: B-Raf Antibody [NBP2-15531] - A. 30 ug PC-12 whole cell extract 7.5 % SDS-PAGE B-Raf antibody [N2C1], Internal dilution: 1:5000](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-15531-img0002.jpg "Western Blot: B-Raf Antibody [NBP2-15531] - A. 30 ug PC-12 whole cell extract 7.5 % SDS-PAGE B-Raf antibody [N2C1], Internal dilution: 1:5000")
![Western Blot: B-Raf Antibody [NBP2-15531] - Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal diluted at 1:5000.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-15531-img0006.jpg "Western Blot: B-Raf Antibody [NBP2-15531] - Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal diluted at 1:5000.")
![Immunoprecipitation: B-Raf Antibody [NBP2-15531] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 ug of B-Raf antibody [N2C1], Internal 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunoprecipitation-NBP2-15531-img0004.jpg "Immunoprecipitation: B-Raf Antibody [NBP2-15531] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 ug of B-Raf antibody [N2C1], Internal 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")