![Western Blot: ASC1 Antibody [NB100-419] - Detection of Human TRIP4/ASC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC1 antibody NB100-419 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASC1-Antibody-Western-Blot-NB100-419-img0003.jpg "Western Blot: ASC1 Antibody [NB100-419] - Detection of Human TRIP4/ASC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC1 antibody NB100-419 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide mapping to a region between residues 75 and 125 of human ASC1 using the numbering given in entry NP_057297.2 (GeneID 9325. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TRIP4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western Blot and immunoprecipitation. Immunohistochemistry has not been tested. *The investigator should determine the optimal working dilution for a specific application. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-419 | Applications | Species |
|---|---|---|
| Wan L, Juszkiewicz S, Blears D et al. Translation stress and collided ribosomes are co-activators of cGAS Molecular cell 2021-07-01 [PMID: 34111399] |
Secondary Antibodies |
Isotype Controls |
Research Areas for ASC1 Antibody (NB100-419)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TRIP4 |
| Entrez |
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| Uniprot |
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![Western Blot: ASC1 Antibody [NB100-419] - Detection of human ASC1 in Western Blot and immunoprecipitation, using NB100-419. Immunoprecipitated ASC1 was detected using an antibody to a distinctly different epitope on ASC1.](http://images.novusbio.com/fullsize/ASC1-Antibody-Western-Blot-NB100-419-img0001.jpg "Western Blot: ASC1 Antibody [NB100-419] - Detection of human ASC1 in Western Blot and immunoprecipitation, using NB100-419. Immunoprecipitated ASC1 was detected using an antibody to a distinctly different epitope on ASC1.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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