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RACK1/GNB2L1 Antibody [Unconjugated]

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Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A20 mouse B cell lymphoma cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RACK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3434) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RACK1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A20 mouse B cell lymphoma cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed ...read more
RACK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat RACK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3434) at 15 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3434-SP
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RACK1/GNB2L1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human RACK1
Met1-Arg317
Accession # P63244
Specificity
Detects human, mouse, and rat RACK1.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GNB2L1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using AF3434.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RACK1/GNB2L1 Antibody [Unconjugated]

  • GNB2L1
  • guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
  • guanine nucleotide binding
  • RACK1
  • Receptor for Activated C Kinase 1
  • Receptor for activated C kinase

Background

The receptor for activated C kinase 1 (RACK1) is highly conserved and has homology to the beta subunit of heterotrimeric G proteins. Initially isolated by its binding of active PKC isoforms, RACK1 is a scaffold protein that promotes the formation of multiprotein complexes that integrate distinct signaling pathways. RACK1 is also a 40S ribosomal subunit constituent, potentially linking these pathways directly to protein translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GNB2L1
Uniprot