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Aquaporin-2 [p Ser269] Antibody

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Western Blot: Aquaporin-2 [p Ser269] Antibody [NBP1-48314] - vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the approx 29k and 37k glycosylated form of the AQP2 protein phosphorylated at ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Aquaporin-2 [p Ser269] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 conjugated to KLH. Accession # P34080
Modification
p Ser269
Specificity
This Antibody is specific for Aquaporin 2 (Ser 269).
Predicted Species
Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AQP2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 3 uL
  • Western Blot 1:1000-1:2000
Application Notes
WB analysis shows bands at 29/37 kDa
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for Aquaporin-2 [p Ser269] Antibody

  • ADH water channel
  • AQP-2
  • AQP-CD
  • aquaporin 2 (collecting duct)
  • aquaporin-2
  • Aquaporin-CD
  • Collecting duct water channel protein
  • MGC34501
  • Water channel protein for renal collecting duct
  • water-channel aquaporin 2
  • WCH-CD

Background

Aquaporin 2 (AQP2) is a membrane protein involved in plasma membrane water transport in the renal collecting duct. This antibody is targeted to a peptide sequence upstream from the phosphorylated region that, upon phosphorylation, can diminish the antibody signal in immunoblots and immunocytochemistry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin-2 Antibody (NBP1-48314) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AQP2
Entrez
Uniprot