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alpha-Fetoprotein/AFP Antibody - BSA Free

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Western Blot: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Analysis in fetal human liver tissue lysate with antibody at (A) 1 ug/mL and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Mouse liver tissue with AFP antibody at 20 ug/mL.
Immunohistochemistry: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Analysis of AFP in mouse liver tissue with AFP antibody at 2.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Rat liver tissue with AFP antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Rat liver tissue with AFP antibody at 10 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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alpha-Fetoprotein/AFP Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the center of human AFP. The immunogen is located within amino acids 340 - 390 of AFP. Amino Acid Squence: CVHEYSRRHPQLAVS
Predicted Species
Porcine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AFP
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
alpha-Fetoprotein/AFP Protein (NBP1-76275PEP)
Publications
Read Publications using
NBP1-76275 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Sheep: (83%), Bovine: (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for alpha-Fetoprotein/AFP Antibody - BSA Free

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Fetoprotein/AFP Antibody (NBP1-76275)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IF/IHC, RIA.


Filter By Application
ICC/IF
(1)
IF/IHC
(2)
RIA
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Fetoprotein/AFP Antibody (NBP1-76275) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AFP
Entrez
Uniprot