| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | PL8-F6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Allophycocyanin/Cy7 |
| Immunogen | Purified human Alkaline Phosphatase/ALPP protein (Uniprot: P05187) |
| Localization | Cytoplasmic and cell surface |
| Marker | Germ Cell Tumor Marker |
| Specificity | Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ALPP |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. |
| Storage | Store at 4C in the dark. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
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![Alkaline Phosphatase/ALPP Antibody (PL8-F6) [Allophycocyanin/Cy7]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Alkaline Phosphatase/ALPP Antibody (PL8-F6) [Allophycocyanin/Cy7]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Alkaline Phosphatase/ALPP Antibody (NBP2-47993APCCY7)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ALPP |
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