I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?

Name: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-47993
Price: 519 USD

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Accepted Answer

None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone	PL8-F6
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	Azide and BSA Free
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP2-47993AF350

Alexa Fluor 405 NBP2-47993AF405

Alexa Fluor 488 NBP2-47993AF488

Alexa Fluor 532 NBP2-47993AF532

Alexa Fluor 594 NBP2-47993AF594

Alexa Fluor 647 NBP2-47993AF647

Alexa Fluor 700 NBP2-47993AF700

Alexa Fluor 750 NBP2-47993AF750

Allophycocyanin NBP2-47993APC

Allophycocyanin/Cy7 NBP2-47993APCCY7

Biotin NBP2-47993B

CoraFluor 1 NBP2-47993CL1

DyLight 350 NBP2-47993UV

DyLight 405 NBP2-47993V

DyLight 488 NBP2-47993G

DyLight 550 NBP2-47993R

DyLight 594 NBP2-47993DL594

DyLight 650 NBP2-47993C

DyLight 680 NBP2-47993FR

DyLight 755 NBP2-47993IR

FITC NBP2-47993F

HRP NBP2-47993H

Janelia Fluor 525 NBP2-47993JF525

Janelia Fluor 549 NBP2-47993JF549

Janelia Fluor 585 NBP2-47993JF585

Janelia Fluor 635 NBP2-47993JF635

Janelia Fluor 646 NBP2-47993JF646

Janelia Fluor 669 NBP2-47993JF669

PE NBP2-47993PE

PE/Atto594 NBP2-47993PEATT594

PE/Cy5.5 NBP2-47993PECY55

PE/Cy7 NBP2-47993PECY7

PerCP NBP2-47993PCP

mFluor Violet 450 SE NBP2-47993MFV450

mFluor Violet 500 SE NBP2-47993MFV500

mFluor Violet 610 SE NBP2-47993MFV610

Catalog# & Formulation	Size	Price
IHC-Prediluted NBP2-48110

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47993AF350
Alexa Fluor 405	NBP2-47993AF405
Alexa Fluor 488	NBP2-47993AF488
Alexa Fluor 532	NBP2-47993AF532
Alexa Fluor 594	NBP2-47993AF594
Alexa Fluor 647	NBP2-47993AF647
Alexa Fluor 700	NBP2-47993AF700
Alexa Fluor 750	NBP2-47993AF750
Allophycocyanin	NBP2-47993APC
Allophycocyanin/Cy7	NBP2-47993APCCY7
Biotin	NBP2-47993B
CoraFluor 1	NBP2-47993CL1
DyLight 350	NBP2-47993UV
DyLight 405	NBP2-47993V
DyLight 488	NBP2-47993G
DyLight 550	NBP2-47993R
DyLight 594	NBP2-47993DL594
DyLight 650	NBP2-47993C
DyLight 680	NBP2-47993FR
DyLight 755	NBP2-47993IR
FITC	NBP2-47993F
HRP	NBP2-47993H
Janelia Fluor 525	NBP2-47993JF525
Janelia Fluor 549	NBP2-47993JF549
Janelia Fluor 585	NBP2-47993JF585
Janelia Fluor 635	NBP2-47993JF635
Janelia Fluor 646	NBP2-47993JF646
Janelia Fluor 669	NBP2-47993JF669
PE	NBP2-47993PE
PE/Atto594	NBP2-47993PEATT594
PE/Cy5.5	NBP2-47993PECY55
PE/Cy7	NBP2-47993PECY7
PerCP	NBP2-47993PCP
mFluor Violet 450 SE	NBP2-47993MFV450
mFluor Violet 500 SE	NBP2-47993MFV500
mFluor Violet 610 SE	NBP2-47993MFV610

Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free

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Description	1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44959). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen	Purified human Alkaline Phosphatase/ALPP protein (Uniprot: P05187)
Localization	Cytoplasmic and cell surface
Marker	Germ Cell Tumor Marker
Specificity	Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Isotype	IgG2b Kappa
Clonality	Monoclonal
Host	Mouse
Gene	ALPP
Purity	Protein A or G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	CyTOF-ready Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml Immunocytochemistry/ Immunofluorescence 1-2 ug/ml Immunofluorescence 0.5 - 1.0 ug/ml Immunohistochemistry Immunohistochemistry-Frozen 0.25 - 0.5 ug/ml Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml Western Blot
Application Notes	Immunohistochemistry (Frozen & Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues. Optimal dilution for a specific application should be determined.
Theoretical MW	70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer	10 mM PBS
Preservative	No Preservative
Concentration	1.0 mg/ml
Purity	Protein A or G purified