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Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (71412) [Unconjugated]

 (6 Publications)
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Activin RIA/ALK-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Activin RIA/ALK-2 Monoclonal Antibody (Catalog # MAB637) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Activin RIA/ALK-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Activin RIA/ALK-2 Monoclonal Antibody (Catalog # MAB637) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
71412
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB637-SP
Carrier Free
MAB637-500
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Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (71412) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIA/ALK‑2
Asp23-Val124
Accession # Q04771
Specificity
Detects human Activin RIA/ALK‑2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACVR1
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB637 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (71412) [Unconjugated]

  • activin A receptor, type I
  • Activin RIA
  • ActivinRIA
  • ACTRI
  • ACVR1
  • ACVR1A
  • ACVRLK2
  • ALK2
  • ALK-2
  • FOP
  • SKR1
  • TSRI

Background

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A), and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type I receptors are highly conserved. Human, mouse and bovine type IA activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type I receptor does not bind activin.

  1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (MAB637)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC-Fr, IHC-P, Neutralization, Western Blot.


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Flow Cytometry
(1)
IHC-Fr
(1)
IHC-P
(1)
Neutralization
(1)
Western Blot
(1)
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Human
(3)
Mouse
(2)
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Showing Publications 1 - 6 of 6.
Publications using MAB637 Applications Species
Senem Aykul, Lily Huang, Lili Wang, Nanditha M. Das, Sandra Reisman, Yonaton Ray et al. Anti-ACVR1 antibodies exacerbate heterotopic ossification in fibrodysplasia ossificans progressiva (FOP) by activating FOP-mutant ACVR1 Journal of Clinical Investigation 6/15/2022 [PMID: 35511419]
Y Kimishima, T Misaka, T Yokokawa, K Wada, K Ueda, K Sugimoto, K Minakawa, K Nakazato, T Ishida, M Oshima, S Koide, K Shide, K Shimoda, A Iwama, K Ikeda, Y Takeishi Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils Nature Communications, 2021-10-26;12(1):6177. 2021-10-26 [PMID: 34702814] (Western Blot, Human) Western Blot Human
HN Arai, F Sato, T Yamamoto, K Woltjen, H Kiyonari, Y Yoshimoto, C Shukunami, H Akiyama, R Kist, A Sehara-Fuj Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate Cell Rep, 2019-10-15;29(3):603-616.e5. 2019-10-15 [PMID: 31618630] (Flow Cytometry, IHC-Fr, Human, Mouse) Flow Cytometry, IHC-Fr Human, Mouse
Sarah J. Hatsell, Vincent Idone, Dana M. Alessi Wolken, Lily Huang, Hyon J. Kim, Lili Wang et al. ACVR1 R206H receptor mutation causes fibrodysplasia ossificans progressiva by imparting responsiveness to activin A Science Translational Medicine 9/2/2015 [PMID: 26333933]
Huse K, Bakkebo M, Oksvold MP, Forfang L, Hilden VI, Stokke T, Smeland EB, Myklebust JH Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: differential effects of BMP-6 and BMP-7. Eur. J. Immunol., 2011-10-18;41(11):3135-45. 2011-10-18 [PMID: 21898381] (Neutralization, Human) Neutralization Human
Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition. J. Biol. Chem., 2007-06-11;282(32):23337-47. 2007-06-11 [PMID: 17562716] (IHC-P, Mouse) IHC-P Mouse

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Bioinformatics

Gene Symbol ACVR1
Uniprot