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ABCG5 Antibody (1B5E10)

Images

 
Western Blot: ABCG5 Antibody (1B5E10) [NBP2-23488] - Western blot analysis of ABCG5 in (1) HL7702, (2) RAJI and (3) Jurkat cell lysate.
Immunohistochemistry: ABCG5 Antibody (1B5E10) [NBP2-23488] - IHC analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.
Flow Cytometry: ABCG5 Antibody (1B5E10) [NBP2-23488] - Flow cytometry analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).
Western Blot: ABCG5 Antibody (1B5E10) [NBP2-23488] - Western blot analysis of ABCG5 in human ABCG5 recombinant protein. (Expected MW is 32.7 kDa)
Western Blot: ABCG5 Antibody (1B5E10) [NBP2-23488] - Western blot analysis of ABCG5 in (1) HEK293 and (2) ABCG5 hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry: ABCG5 Antibody (1B5E10) [NBP2-23488] - IHC analysis of paraffin-embedded human cervical cancer cancer tissues with DAB staining.

Product Details

Summary
Product Discontinued
View other related ABCG5 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-23488
    • Availability
      Product Discontinued

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ABCG5 Antibody (1B5E10) Summary

Immunogen
Partial recombinant human ABCG5 (between residues 300-500) expressed in E. coli [UniProt Q9H222].
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ABCG5
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:500 - 1:2000
Control
Jurkat Whole Cell Lysate
Raji Whole Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.5% proprietary protein stabilizer
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for ABCG5 Antibody (1B5E10)

  • ATP-binding cassette sub-family G member 5
  • ATP-binding cassette, sub-family G (WHITE), member 5
  • ATP-binding cassette, subfamily G, member 5
  • sterolin 1
  • sterolin-1
  • STSL

Background

ABCG5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCG5 is a member of the White subfamily. It functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. ABCG5 is expressed in a tissue-specific manner in the liver, colon, and intestine. Mutations in this protein may contribute to sterol accumulation and atheroschlerosis, and they have been observed in patients with sitosterolemia (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ABCG5 Products

Array NBP2-23488

Research Areas for ABCG5 Antibody (NBP2-23488)

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Blogs on ABCG5.

ABCG8: Cholesterol's Fate
The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

Exploring how ABCG8 Affects Heart Disease
The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th...  Read full blog post.

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Bioinformatics

Gene Symbol ABCG5
Entrez
Uniprot