SREBP2 Antibody (1D2) - BSA Free

Sterol regulatory element-binding proteins (SREBPs) compose a family of transcriptional factors that regulate the expression of various genes required for the synthesis of phospholipids, fatty acids, and cholesterol. Out of the three isoforms present (SREBP-1a, SREBP-1c and SREBP-2), SREBP-2 has been implicated more directly in the synthesis of cholesterol during metabolism. Similar to SREBP-1a and SREBP-1c, SREBP-2 acts by first binding to sterol regulatory DNA sequences, and subsequently up-regulating the synthesis of enzymes involved in sterol biosynthesis. SREBP-2 is expressed in both the cytoplasm and nucleus on a subcellular level, depending on the state of the protein. It has been known to interact and form a complex with the SCAP protein at the ER, in turn inhibiting interaction between SCAP and SEC24B. This interaction prevents the cleavage of SREBP-2, which results in the synthesis of cholesterol and uptake of LDL despite high levels of present sterols. In recent research, new functions for the SREBP family have offered insight on the effect of lipid metabolism on various pathophysiological diseases (cancers, steatosis and immunity, PMID: 22154484).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 6 applications: ChIP, IHC-P, IP, Immunohistochemistry, In Vivo, WB.

Showing Publications 1 - 10 of 20. Show All 20 Publications. Collapse Publications.

Publications using NBP1-54446	Applications	Species
Chen F, Matsuda A, Budinger GRS et al. Hypercapnia increases ACE2 expression and pseudo-SARS-CoV-2 entry in bronchial epithelial cells by augmenting cellular cholesterol Frontiers in immunology 2023-10-12 [PMID: 37901225] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
Fowler JWM, Boutagy NE, Zhang R et al. SREBP2 regulates the endothelial response to cytokines via direct transcriptional activation of KLF6 Journal of lipid research 2023-07-10 [PMID: 37437844] (ChIP, Human)	ChIP	Human
Soundararajan A, Wang T, Sundararajan R et al. Multiomics analysis reveals the mechanical stress-dependent changes in trabecular meshwork cytoskeletal-extracellular matrix interactions Frontiers in Cell and Developmental Biology 2022-09-13 [PMID: 36176278]
Tanton H, Sewastianik T, Seo HS et al. A novel beta-catenin/BCL9 complex inhibitor blocks oncogenic Wnt signaling and disrupts cholesterol homeostasis in colorectal cancer Science advances 2022-04-29 [PMID: 35486727] (WB)	WB
Ghaffari, S, Jang, E Et al. Endothelial HMGB1 Is a Critical Regulator of LDL Transcytosis via an SREBP2-SR-BI Axis. Arterioscler Thromb Vasc Biol 2021-01-01 [PMID: 33054399] (IHC-P, In Vivo, Human, Mouse)	IHC-P, In Vivo	Human, Mouse
Yang R, Zhao Y, Gu Y et al. Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma Oncogene 2020-08-27 [PMID: 32855525] (WB)	WB
Du X, Kazim AS, Dawes IW et al. The AAA ATPase VPS4/SKD1 regulates endosomal cholesterol trafficking independently of ESCRT-III.. Traffic. 2013-01-01 [PMID: 23009658] (WB, Human)	WB	Human
Du X, Kazim AS, Brown AJ, Yang H. An essential role of Hrs/Vps27 in endosomal cholesterol trafficking.. Cell Rep. 2012-01-26 [PMID: 22832105] (WB, Human) Details: SREBP2 antibody (clone 1D2) was used for WB analysis of lysates from HeLa cells that were transfected with siNPC1, siHrs, or a universal control siCtrl, and were then stimulated with 50ug/ml of LDL (Figure 3C).	WB	Human
Luu W, Sharpe LJ, Stevenson J, Brown AJ et al. Akt acutely activates the cholesterogenic transcription factor SREBP-2.. Biochim Biophys Acta. 2012-02-01 [PMID: 22005015] (WB, Human)	WB	Human
Song BL, DeBose-Boyd RA. Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl coenzyme a reductase stimulated by delta- and gamma-tocotrienols.. J Biol Chem. 2006-09-01 [PMID: 16831864] (WB, Human)	WB	Human
Song BL, Javitt NB, DeBose-Boyd RA. Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol.. Cell Metab. 2005-03-01 [PMID: 16054061] (WB, Human) Details: SREBP2 antibody (clone 1D2) was used at 5ug/ml concentration in WB application on nuclear/membrane fractions of SV-589 cells (an immortalized line of human fibroblasts expressing the SV40 large T antigen) that were exposed to medium A containing 10% lipoprotein-deficient serum/LPDS, 50 uM compactin, 10 mM mevalonate, and different different concentrations of 25-hydroxycholesterol, lanosterol, or 24,25-dihydrolanosterol (Figure 2A).	WB	Human
Sun LP, Li L, Goldstein JL, Brown MS. Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro.. J Biol Chem. 2005-07-15 [PMID: 15899885] (WB, Human)	WB	Human
Adams CM, Reitz J, De Brabander JK et al. Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs.. J Biol Chem. 2004-12-10 [PMID: 15452130] (WB, Human)	WB	Human
Zhang Y, Hillgartner FB. Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens.. J Nutr. 2004-09-01 [PMID: 15333705] (WB, Chicken) Details: SREBP2 antibody (clone 1D2) was used for WB analysis of nuclear and membrane fractions from livers of 10- 12 days old chickens subjected to 24 hours of starvation followed by feeding of high-carbohydrate, low-fat diet for 0, 5, and 24 hours (FIGURE 3). The antibody was also used for EMSA /GS (gel mobility shift assays) under similar experimental conditions on nuclear extracts from chick's liver tissue (FIGURE 5).	WB	Chicken
Demoulin JB, Ericsson J, Kallin A et al. Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins.. J Biol Chem. 2004-08-20 [PMID: 15213220] (IP, WB, Human)	IP, WB	Human
Sever N, Yang T, Brown MS et al. Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain.. Mol Cell. 2003-01-01 [PMID: 12535518] (WB, Human)	WB	Human
Janowski BA. The hypocholesterolemic agent LY295427 up-regulates INSIG-1, identifying the INSIG-1 protein as a mediator of cholesterol homeostasis through SREBP.. Proc Natl Acad Sci U S A. 2002-10-01 [PMID: 12242342] (WB, Human) Details: SREBP2 antibody (clone 1D2) was used for WB analysis of lysates from HepG2 cells that were subjected to medium containing 1ug/ml of 25-hydroxycholesterol/25HC and / or LY295427, a known antagonist of the cholesterol-homeostatic effects of 25-HC.	WB	Human
Yabe D, Brown MS, Goldstein JL. Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins.. Proc Natl Acad Sci U S A. 2002-10-01 [PMID: 12242332] (WB, Human) Details: SREBP2 antibody (clone 1D2) was used at 1-5ug/ml concentration in WB application on lysates of SRD-13A cells transfected with human pTK-HSV-BP-2 encoding wild-type HSV-tagged human SREBP-2 . The antibody detected the cleaved nuclear and uncleaved precursor forms of SREBP2 protein. See full text for detailed protocols and data images.	WB	Human
Yin L, Zhang Y, Hillgartner FB. Sterol regulatory element-binding protein-1 interacts with the nuclear thyroid hormone receptor to enhance acetyl-CoA carboxylase-alpha transcription in hepatocytes.. J Biol Chem. 2002-05-31 [PMID: 11907029] (WB, Chicken)	WB	Chicken
Janowski BA, Shan B, Russell DW. The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols.. J Biol Chem. 2001-11-30 [PMID: 11577112] (WB, Human)	WB	Human
Show All 20 Publications. Collapse Publications.

Array NBP1-54446

• SREBP2 Antibodies
• SREBP2 Kit
• SREBP2 Lysate
• SREBP2 Proteins