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Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit Summary

Background
The Quantikine Human PCSK9 immunoassay is a 4.5 hour solid phase ELISA designed to measure PCSK9 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human PCSK9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural PCSK9 showed linear curves that were parallel to the standard curves obtained using the... Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring PCSK9.
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Specificity
Natural and recombinant human PCSK9. This assay recognizes free and LDLR-bound PCSK9.
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
PCSK9

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DPC900.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit

  • 170 kDa melanoma membrane-bound gelatinase
  • DKFZp686G13158
  • DPPIV
  • EC 3.4.21.-
  • FAP
  • FAPA
  • Fibroblast Activation Protein alpha
  • fibroblast activation protein, alpha
  • Integral membrane serine protease
  • Seprase
  • vibronectin

Background

Proprotein convertase subtilisin kexin 9 (PCSK9), also named neural apoptosis-regulated convertase 1 (NARC-1), is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. The full-length protein has 692 amino acids, including a signal peptide, a pro- domain, and a catalytic domain. PCSK9 is highly expressed in the liver, intestine, and kidney. It is initially synthesized as a soluble 74 kDa precursor protein. In the endoplasmic reticulum, it undergoes autocatalytic intramolecular cleavage to generate a 14 kDa pro- domain and a 60 kDa catalytic domain. These two domains remain associated when PCSK9 is secreted outside the cells (1-3). The primary physiologic function of PCSK9 is to mediate the degradation of low density lipoprotein receptor (LDL R). Early observations indicated that gain-of-function missense mutations in the PCSK9 gene can cause an autosomal dominant form of hypercholesterolemia (4, 5). The expression of PCSK9 was observed to be up-regulated by the sterol regulatory element binding proteins (SREBPs), a family of transcription factors that are responsible for the upregulation of genes involved in cholesterol and fatty acid metabolism, such as the LDL R gene (6, 7). Further experimental evidence revealed that in mice, when the PCSK9 gene was knocked out, the number of LDL R in hepatocytes increased, whereas when PCSK9 was over-expressed, the amount of LDL R protein was reduced in the liver (8, 9). In humans, genetic analyses have shown that individuals who have nonsense or loss-of-function mutations in the PCSK9 gene have significantly lower plasma LDL cholesterol levels (10, 11). These investigations clearly indicated that PCSK9 plays a key role in reducing the hepatic LDL R levels. Recently, the underlying mechanism has been uncovered: under normal physiologic conditions, the LDL R is internalized on the cell surface and directed to the endosomes in order to be recycled back to the cell surface. PCSK9 binds to the EGF domain of the LDL R and prevents LDL R from being sorted to the endosomes. Instead, the PCSK9/LDL R complex is redistributed to the lysosomes for degradation (12-14). As such, PCSK9 regulates the amount of LDL R in the circulation and modulates cholesterol levels. Serum PCSK9 concentrations have been found to be directly associated with cholesterol levels (15, 16). Since individuals with loss-of-function PCSK9 mutations have strikingly reduced risk of coronary heart diseases, PCSK9 has become an attractive drug target in recent years (17, 18). One approach is to generate small molecules that are able to interfere with PCSK9 autoactivation and its interaction with LDL R. Other approaches aiming to reduce the amounts of PCSK9 in the circulation, such as small interfering RNAs (siRNAs), have also shown promise (19, 20).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Fibroblast Activation Protein alpha/FAP (DPC900)(51)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Transgenic Mouse.


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Human
(45)
Mouse
(3)
Rat
(1)
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(1)
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(1)
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Showing Publications 1 - 10 of 51. Show All 51 Publications.
Publications using DPC900 Applications Species
Oguri, N;Gi, T;Nakamura, E;Furukoji, E;Goto, H;Maekawa, K;Tsuji, AB;Nishii, R;Aman, M;Moriguchi-Goto, S;Sakae, T;Azuma, M;Yamashita, A; Expression of fibroblast activation protein-? in human deep vein thrombosis Thrombosis research 2024-06-25 [PMID: 38955058] (Human) Human
Zhang, B;Chuang, GY;Biju, A;Biner, D;Cheng, J;Wang, Y;Bao, S;Chao, CW;Lei, H;Liu, T;Nazzari, AF;Yang, Y;Zhou, T;Chen, SJ;Chen, X;Kong, WP;Ou, L;Parchment, DK;Sarfo, EK;SiMa, H;Todd, JP;Wang, S;Woodward, RA;Cheng, C;Rawi, R;Mascola, JR;Kwong, PD; Cholesterol reduction by immunization with a PCSK9 mimic Cell reports 2024-05-30 [PMID: 38819987] (Human) Human
Bestas, B;Wimberger, S;Degtev, D;Madsen, A;Rottner, AK;Karlsson, F;Naumenko, S;Callahan, M;Touza, JL;Francescatto, M;Möller, CI;Badertscher, L;Li, S;Cerboni, S;Selfjord, N;Ericson, E;Gordon, E;Firth, M;Chylinski, K;Taheri-Ghahfarokhi, A;Bohlooly-Y, M;Snowden, M;Pangalos, M;Nuttall, B;Akcakaya, P;Sienski, G;Maresca, M; A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo Nature communications 2023-09-06 [PMID: 37673883] (Mouse) Mouse
Ballantyne, CM;Ditmarsch, M;Kastelein, JJ;Nelson, AJ;Kling, D;Hsieh, A;Curcio, DL;Maki, KC;Davidson, MH;Nicholls, SJ; Obicetrapib plus ezetimibe as an adjunct to high-intensity statin therapy: A randomized phase 2 trial Journal of clinical lipidology 2023-05-31 [PMID: 37277261] (Human) Human
A Larrea-Seb, C Trenti, S Jebari-Ben, S Bertolini, S Calandra, EA Negri, E Bonelli, A Benito-Vic, L Uraga-Grac, C Martín, T Fasano Functional Characterization of p.(Arg160Gln) PCSK9 Variant Accidentally Found in a Hypercholesterolemic Subject International Journal of Molecular Sciences, 2023-02-07;24(4):. 2023-02-07 [PMID: 36834740] (Human) Human
Atreya MR, Cvijanovich NZ, Fitzgerald JC et al. Detrimental effects of PCSK9 loss-of-function in the pediatric host response to sepsis are mediated through independent influence on Angiopoietin-1 Research square 2023-02-03 [PMID: 36778250] (Human) Human
MK ?widerska, A Mostowska, D Skrypnik, PP Jagodzi?sk, P Bogda?ski, AE Grzegorzew Energy Homeostasis Gene Nucleotide Variants and Survival of Hemodialysis Patients-A Genetic Cohort Study Journal of Clinical Medicine, 2022-09-18;11(18):. 2022-09-18 [PMID: 36143124] (Human) Human
F Spannella, F Giulietti, R Galeazzi, A Passarelli, S Re, C Di Pentima, M Allevi, P Magni, R Sarzani Plasma Levels of Proprotein Convertase Subtilisin/Kexin Type 9 Are Inversely Associated with N-Terminal Pro B-Type Natriuretic Peptide in Older Men and Women Biomedicines, 2022-08-12;10(8):. 2022-08-12 [PMID: 36009507] (Human) Human
LS Silva-Berm, A Vargas-Vil, CA Sánchez-Va, AC Palacio, AF Buitrago, CO Mendivil Peri-event plasma PCSK9 and hsCRP after an acute myocardial infarction correlate with early deterioration of left ventricular ejection fraction: a cohort study Oncogene, 2022-07-21;21(1):61. 2022-07-21 [PMID: 35864531] (Human) Human
J Baran, ? Niewiara, J Podolec, M Siedli?ski, E Józefczuk, A Bernacik, R Badacz, T Przew?ocki, P Pieni??ek, K ?mudka, J Legutko, A Kab?ak-Zie Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes Oncogene, 2022-06-17;9(6):. 2022-06-17 [PMID: 35735822] (Human) Human
Show All 51 Publications.

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Bioinformatics

Gene Symbol PCSK9