Our customer would like to consult something about the ABCA1 antibody with the Cat No. NB400-105. In the datasheet, it showed this protein expressed very low in most cell types. The customer may test the mouse liver tissues, and he doesn’t know the expression level of ABCA1 in this tissue. Is it necessary to induce the expression via LXR treatment to get enough protein expressed? If he doesn’t induce the expression, how much protein loaded was suitable as recommended?

Our own Western blot data for NB400-105 was generated by treating RAW cells with 9-cis-retinoic acid and 22R-hydroxycholesterol, which are both known to induce expression of ABCA1 in macrophages. 40ug of protein was loaded on to the gel in this instance. Unfortunately we do not have any testing data from mouse liver tissues using this particular antibody, however the data for another of our ABCA1 antibodies may be of interest to your customer: NB100-2068. The Western blot data for this product was generated using mouse liver tissue, and a clear band can be seen at the expected molecular weight in wild type tissue. We loaded 30ug of protein when running this Western blot, however, your customer may need to optimise the staining procedure for their sample type.

Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?

Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.

We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?

Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.

I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.

Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.

To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?

The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.

We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?

ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.

Does this product contain any BSA?

No, this antibody does not have any BSA in its formulation.

I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?

The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.

Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?

The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.

Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?

To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.

Can i use DTT instead of Beta- mercaptoethanol?

Either DTT or 2-mercaptoethanol is routinely used in SDS-PAGE sample buffer (reducing gel electrophoresis). Except that 2-mercaptoethanol is a stronger reducing agent and is mostly frequently used for that purpose.

Have you tested abca1 antibody(catalog # NB400-105) on hepatic Abca1 expression?

We do not currently have any testing data where we have used NB400-105 with hepatic samples; all of our testing data for NB400-105 can be seen on the product page for this antibody. Should your customer be interested, we do show murine liver data with the following product: NB100-2068.

For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?

For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.

ABCA1 Antibody - BSA Free

Name: ABCA1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB400-105
Rating: 4.3 (7 reviews)

(414 Publications)

(7 Reviews)

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Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

Orthogonal Strategies: Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). ...read more

Genetic Strategies: HSKMCs were transfected with scrambled siRNA (scr siRNA) or ABCA1 siRNA. The cells were lysed and ABCA1 (A) protein levels were quantified by immunoblotting. Image collected and cropped by ...read more

Biological Strategies: Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total ...read more

Biological Strategies: HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior to being fixed for 10 minutes using 10% formalin and then ...read more

Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more

ABCA-1 FITC conjugated antibody of human adipose tissue macrophage subsets by flow cytometry. Image from verified customer review.

Association between expression of ABCA1 and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC in tissue microarray. Representative image of ovarian ...read more

ABCA1 in human primary fibroblasts in culture. 10 ug of total protein. 7.5% TGX gel. Blocking 5% milk in PBST 1h RT. 1:1000 primary ab in BSA 3% PBST O/N at 4C. Secondary 1:5000 HRP 1h RT. Arrow shows around 250 kDa. WB ...read more

ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Biological Strategies: Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate.

Prostates of LXR mutant mice accumulate cholesterol esters through inappropriate LXR target genes regulation. Total protein lysates of WT and LXR null mice under normal or high cholesterol diet were analyzed by western ...read more

miR-33b reduces cellular cholesterol efflux and serum HDL-C levels. Western blotting for ABCA1 (using NB400-105) and ABCG1 proteins in peritoneal macrophages from WT and KI+/+ mice. Representative images are shown. ...read more

miR-33b regulates ABCA1 and SREBP-1. Analysis for ABCA1, SREBP-1, CPT1a, and AMPK alpha protein levels in the livers of WT, KI+/-, and KI+/+ mice. Representative images are shown. TF2B and beta-actin were used as ...read more

Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.

Liver mRNA & protein expression analysis of chow-fed Osbpl8KO mice.A: qPCR analysis of the quantity of the mRNAs identified at the bottom in chow-fed KO females (open bars) & males (closed bars). The mRNAs were ...read more

Protein expressions of ABCA1, SR-BI & ABCG1 in liver & macrophage by Western blot. Simvastatin increased the expressions of ABCA1 & ABCG1 in liver & ABCA1 in macrophage, Both L-4F & the combination group improved the ...read more

ABCA1, ABCG1 & SR-BI protein expressions. a & b Hepatic protein expressions of ABCA1, ABCG1 & SR-BI were significantly decreased in COMT−/− mice at GD 18.5, compared to C57BL/6 J mice. Decreased hepatic ABCA1 ...read more

Effects of 13-HODE & LA in the presence & absence of PPAR alpha & PPAR gamma selective antagonists on molecular markers of cholesterol homeostasis in RAW264.7 macrophages. RAW264.7 cells were pre-treated without or ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Upregulation of ABCA1 & ABCG1 & (B) enhancement of cholesterol efflux by purple Perilla frutescens extracts (PPE) in 50 μg/ml Cu2+-oxidized low-density ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - 6‐Dihydroparadol increases A) ABCA1 & B) ABCG1, but not C) SR‐BI protein levels in cholesterol‐loaded THP‐1‐derived macrophages. THP‐1 cells were ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Akt inhibition suppresses mTORC1 activity.BHK-ABCA1 cells were induced overnight with mifepristone (10 nM) & then incubated with DEBC, LY294002 or rapamycin at ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Reversal of hepatic steatosis by reduction of SREBP-1 levels.(a) Western blotting analysis of SREBP-1 & ABCA1 levels in livers of miR-33+/+Srebf1+/+, ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - 6‐Dihydroparadol increases A) ABCA1 & B) ABCG1 protein stability. THP‐1 cells were differentiated as described in Figure 2. Then cells were loaded with unlabeled ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Ligand activation of LXR inhibits LPS-induced MAP kinase activation through Abca1 induction.(A–D) Bone marrow-derived macrophages from Lxr alpha −/−Lxr beta ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Western Blotting for ABCA1, as detailed in Supplementary Protocol #5 (Additional file 1). Caco-2 cells cultivated on filter inserts were treated for 48 h with ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Expression of ABCA1 & CROT in livers & RIP140 in macrophages is elevated in miR-33−/−Apoe−/− mice compared with miR-33+/+Apoe−/− mice. A, Quantitative ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Basal ABCA1 expression & function in patient-derived iRPEs. (A) Representation of patient-derived iPSC lines & genotypes harboring polymorphisms in ABCA1 that are ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - A–C) Effects of 6‐dihydroparadol & specific proteasomal, lysosomal, & calpain inhibitors on the ABCA1 protein levels. THP‐1 cells were differentiated as ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Suppressive effects of Ucn1 on foam cell formation & related protein expression in human monocyte-derived macrophages.Human monocytes were incubated for 7 days with ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Effect of evodiamine on ABCA1 transcription & the degradation rate of ABCA1 protein. (a) Differentiated THP-1 macrophages were incubated with 10 μM evodiamine or ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - miR-33 deficiency improved cholesterol efflux in macrophages. A, Quantitative real-time PCR analysis of Abca1 & Abcg1 in macrophages from miR-33+/+Apoe−/− & ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Srebf1 is a miR-33 target gene.(a) Relative changes in lipid metabolism-related genes in the livers of miR-33−/− mice compared with miR-33+/+ mice fed NC at 16 ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Chemical structure, (B) cytotoxicity of alpha -asarone, (C) enhancement of cholesterol efflux by alpha -asarone, (D & E) upregulation of ABCA1 & ABCG1 by ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Bilirubin significantly decreases expression of ATP‐binding cassette transporter A1 (ABCA1) protein in THP‐1 macrophages treated with LXR agonist (TO901317) to ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Induction of cholesterol efflux attenuates the enhancement in HIV-1infectivity in NPCD55 cells. (A) Normal & NPCD55 cells were treated with 5 μM TO-901317 at 24 h ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Effects of apabetalone on ABCA1 expression & carboplatin response in ovarian cancer cells. (A) Apabetalone treatment reduced expression of ABCA1 in OVCAR-5 & OVCAR-5 ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Expression of ATP‐binding cassette transporter A1 (ABCA1) protein in THP‐1 macrophages treated with bilirubin & in peripheral blood mononuclear cells (PBMCs) ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Time course response of ABCA1 induction by pioglitazone, & (B) enhancement of ABCA1 & (C) peroxisome proliferator-activated receptor gamma (PPAR gamma ) by ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - LXR agonist-stimulated ABCA1 expression & function in patient-derived iRPEs. Patient-derived iRPE lines were stimulated for 16 h with LXR agonist & are relative to ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Akt inhibition by LY294002 or Akt1/2 also enhances cholesterol efflux to apoA-I from ABCA1-expressing BHK cells.A) BHK cells were induced as in Fig. 1 & then ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Generation, differentiation & analysis of ABCA1-deficient iRPEs. (A) Immunofluorescence staining for ZO-1 (red) in 4-week cultured iRPEs. Nuclei were stained with ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Akt inhibition enhances cholesterol efflux to apoA-I from wt MEFs, but not from TSC2-/- MEFs where mTORC1 is constitutively activated.A) Mouse embryonic fibroblasts ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Akt inhibition by DEBC enhances cholesterol efflux to apoA-I specifically from ABCA1-expressing BHK cells.A) BHK-ABCA1 cells were induced with mifepristone (10 nM) ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - LXRs control expression of genes involved in cholesterol homeostasis & fatty acid synthesis in MPECs.(A) qPCR analysis of Abca1, Abcg1 & Idol levels in WT (+/+) & ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Anti-ApoA-1 IgGs upregulate ABCA1. ABCA1 was increased after 16 & 24 h of anti-ApoA-1 IgG exposure to HMDM at the mRNA level as revealed by RT-PCR (a) as well as at ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - ABCA1 protein expression is upregulated in ovarian cancer cells with acquired carboplatin resistance. ABCA1 expression in OVCAR-5 (A) & CaOV3 (C) & carboplatin ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Evodiamine enhances apo A1-mediated ChE from THP-1 macrophages & increases ABCA1 protein level. (a) Differentiated THP-1 cells were loaded with [3H]-cholesterol ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Functional miR-33 responsive elements in the 3′UTR of ATP8B1 & ABCB11A,B. Conserved sequences in the 3′UTR of ATP8B1 & ABCB11 are partially complementary to ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Gas6 treatment enhances expression of LXR alpha & LXR beta & their target genes in BMDM.Mouse BMDM were stimulated with 400 ng/ml Gas6, 1 μM T0901317, ...read more

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Time course response of ABCA1 induction by TO-091317 & (B) upregulation of ABCA1 by purple Perilla frutescens extracts (PPE), & (C) enhancement of liver X ...read more

Product Details

Summary

Reactivity	Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, RbSpecies Glossary
Applications	WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IP, WB, B/N, ChIP, Dual ISH-IHC, Func, GS, KD, KO
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Validated by:

Biological Strategies

Genetic Strategies

Orthogonal Strategies

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB400-105AF350

Alexa Fluor 405 NB400-105AF405

Alexa Fluor 488 NB400-105AF488

Alexa Fluor 532 NB400-105AF532

Alexa Fluor 594 NB400-105AF594

Alexa Fluor 647 NB400-105AF647

Alexa Fluor 700 NB400-105AF700

Alexa Fluor 750 NB400-105AF750

Allophycocyanin NB400-105APC

Allophycocyanin/Cy7 NB400-105APCCY7

Biotin NB400-105B

CoraFluor 1 NB400-105CL1

DyLight 350 NB400-105UV

DyLight 405 NB400-105V

DyLight 488 NB400-105G

DyLight 550 NB400-105R

DyLight 594 NB400-105DL594

DyLight 650 NB400-105C

DyLight 680 NB400-105FR

DyLight 755 NB400-105IR

FITC NB400-105F

HRP NB400-105H

Janelia Fluor 525 NB400-105JF525

Janelia Fluor 549 NB400-105JF549

Janelia Fluor 646 NB400-105JF646

Janelia Fluor 669 NB400-105JF669

PE NB400-105PE

PE/Atto594 NB400-105PEATT594

PE/Cy5.5 NB400-105PECY55

PE/Cy7 NB400-105PECY7

PerCP NB400-105PCP

mFluor Violet 450 SE NB400-105MFV450

mFluor Violet 500 SE NB400-105MFV500

mFluor Violet 610 SE NB400-105MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
BSA Free NB400-105SS

Novus Biologicals offers 100% Guarantee

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB400-105AF350
Alexa Fluor 405	NB400-105AF405
Alexa Fluor 488	NB400-105AF488
Alexa Fluor 532	NB400-105AF532
Alexa Fluor 594	NB400-105AF594
Alexa Fluor 647	NB400-105AF647
Alexa Fluor 700	NB400-105AF700
Alexa Fluor 750	NB400-105AF750
Allophycocyanin	NB400-105APC
Allophycocyanin/Cy7	NB400-105APCCY7
Biotin	NB400-105B
CoraFluor 1	NB400-105CL1
DyLight 350	NB400-105UV
DyLight 405	NB400-105V
DyLight 488	NB400-105G
DyLight 550	NB400-105R
DyLight 594	NB400-105DL594
DyLight 650	NB400-105C
DyLight 680	NB400-105FR
DyLight 755	NB400-105IR
FITC	NB400-105F
HRP	NB400-105H
Janelia Fluor 525	NB400-105JF525
Janelia Fluor 549	NB400-105JF549
Janelia Fluor 646	NB400-105JF646
Janelia Fluor 669	NB400-105JF669
PE	NB400-105PE
PE/Atto594	NB400-105PEATT594
PE/Cy5.5	NB400-105PECY55
PE/Cy7	NB400-105PECY7
PerCP	NB400-105PCP
mFluor Violet 450 SE	NB400-105MFV450
mFluor Violet 500 SE	NB400-105MFV500
mFluor Violet 610 SE	NB400-105MFV610

ABCA1 Antibody - BSA Free Summary

Immunogen	Partial peptide sequence (within residues 1100-1300) of human ABCA1 Antibody [UniProt# O95477]. Actual immunogen sequence is proprietary information.
Localization	Astrocyte Marker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	ABCA1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Block/Neutralize reported in scientific literature (PMID 30821416)
Chromatin Immunoprecipitation (ChIP)
Chromatin Immunoprecipitation reported in scientific literature (PMID 19515742)
Dual RNAscope ISH-IHC
ELISA reported in scientific literature (PMID 18541924)
Flow Cytometry 1:400. Use reported in scientific literature (PMID 21501868)
Gel Super Shift Assays reported in scientific literature (PMID 15684432)
Gel Supershift Assay
Immunoblotting reported in scientific literature (PMID 27599291)
Immunocytochemistry/ Immunofluorescence 1:100. Use reported in scientific literature (PMID 21501868)
Immunohistochemistry 1:200
Immunohistochemistry-Frozen
Immunohistochemistry-Paraffin 1:200
Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 21846716; 21106520)
Knockdown Validated reported in scientific literature (PMID 31666189)
Knockout Validated reported in scientific literature (PMID 32273567)
PCR reported in scientific literature (PMID 27406916)
Simple Western 1:50
Western Blot 1:500

Application Notes

Western Blot band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: separated by Charge, antibody dilution of 1:50Separated by Charge.

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control

Raw 264.7 Whole Cell - T0901317 treated Assay Kit
THP-1 TO901317 Treated / Untreated Cell Lysate

Control Peptide

ABCA1 Protein (NB400-105PEP)

Reviewed Applications

Read 7 Reviews rated 4.3

using
NB400-105 in the following applications:

Western Blot
Immunofluorescence

Publications

Read Publications using
NB400-105 in the following applications:

B/N
1 publication
Block/Neutralize
1 publication
Chemotaxis
1 publication
ELISA
1 publication
EMSA
1 publication
FLOW
3 publications
Flow
1 publication
IB
1 publication
ICC/IF
23 publications
IF/IHC
19 publications
IHC
1 publication
IHC-Fr
6 publications
IHC-P
9 publications
IP
9 publications
Immunocytochemistry/ Immunofluorescence
1 publication
Immunohistochemistry
1 publication
Immunohistochemistry-Frozen
1 publication
KD
1 publication
KO
1 publication
WB
210 publications
Western Blot
1 publication

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24612239). Rabbit reactivity reported in scientific literature (PMID: 26444796). Chinese Hamster reactivity reported in scientific literature (PMID: 27902765). Primate reactivity reported in scientific literature (PMID: 25440061). Equine reactivity reported in scientific literature (PMID: 26711702).

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1 mg/ml
Purity	Immunogen affinity purified

Alternate Names for ABCA1 Antibody - BSA Free

ABC1
ABC-1
ABC1ATP-binding cassette transporter A1
ABCA1
ATP-binding cassette 1
ATP-binding cassette transporter 1
ATP-binding cassette, sub-family A (ABC1), member 1
CERP
Cholesterol efflux regulatory protein
EC 2.7.7.8
EC 2.8.1.8
EC 3.6.3
EC 3.6.3.41
FLJ14958
HDLDT1
membrane-bound
MGC164864
MGC165011
TGD
TGDATP-binding cassette sub-family A member 1

Background

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ICC/IF, IHC, IHC-P, IP, WB

RFC2 Antibody
(OTI2B2)

NBP2-45570

Immunohistochemistry RFC2 Antibody (OTI2B2)

Species: Hu, Mu, Rt

Applications: Flow, IHC, IHC-P, WB

RFC1 Antibody
[Unconjugated]

AF6457

Species: Hu

Applications: WB

RFC4 Antibody
(OTI1A8)

NBP2-45946

Immunohistochemistry RFC4 Antibody (OTI1A8)

Species: Ca, Hu, Pm, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, WB

PPAR gamma/NR1C3
Antibody - BSA Free

NBP2-22106

Immunohistochemistry-Paraffin PPAR gamma/NR1C3 Antibody - BSA Free

(2 Reviews)

(12 Publications)

Species: Hu, Mu, Rt

Applications: IHC, IHC-P, IP, WB

Apolipoprotein
E/ApoE Antibody (WUE- ...

NB110-60531

Western Blot Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Flow Cytometry Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

(2 Reviews)

(41 Publications)

Species: Hu, Mu, Rt(-)

Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB

ABCB6 Antibody

NBP3-41339

Immunohistochemistry-Paraffin ABCB6 Antibody

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, WB

ABCA4 Antibody
(3F4)

NBP1-30032

Immunohistochemistry ABCA4 Antibody (3F4)

(1 Review)

(5 Publications)

Species: Bv, Ca, Fe, Hu, Mu, Xp

Applications: ICC/IF, IHC, IHC-Fr, KO, WB

CD36 Antibody -
BSA Free

NB400-144

Immunohistochemistry CD36 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence CD36 Antibody - BSA Free

(9 Reviews)

(136 Publications)

Species: Av, Bv, Hu, Mu, Po, Pm, Rb, Rt

Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB

LDLR Antibody
[Unconjugated]

AF2255

Western Blot LDLR Antibody [Unconjugated]

(47 Publications)

Species: Mu

Applications: Block, CyTOF-ready, Flow, IHC, WB

CETP Antibody
(ATM192) - BSA Free

NB300-558

Western Blot CETP Antibody (ATM192) - BSA Free

(1 Publication)

Species: Bv, Hu, Rb

Applications: ELISA, ICC/IF, IP, WB

PPAR alpha/NR1C1
Antibody (3B6/PPAR) - ...

NB300-537

Western Blot PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

(1 Review)

(16 Publications)

Species: Bv, Hu, Mu, Rb, Rt

Applications: ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB

Insulin Antibody
(182410) [Unconjugate ...

MAB1417

Immunohistochemistry Insulin Antibody (182410) [Unconjugated]

(25 Publications)

Species: Bv, Hu, Mu

Applications: CyTOF-ready, ICC, IHC, ICFlow

TNF-alpha
[Unconjugated]

210-TA

(3 Reviews)

(822 Publications)

Species: Hu

Applications: BA

Lipoprotein
Lipase/LPL Antibody [ ...

AF7197

Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]

Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]

(12 Publications)

Species: Hu, Mu

Applications: ICC, IHC, WB

Publications for ABCA1 Antibody (NB400-105)(414)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Canine, Chicken, Chinese Hamster, Hamster, Mustelid, Porcine, Rabbit.

We have publications tested in 21 applications: B/N, Block/Neutralize, Chemotaxis, ELISA, EMSA, FLOW, Flow, IB, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, KD, KO, WB, Western Blot.

Showing Publications 1 - 10 of 414. Show All 414 Publications.

Publications using NB400-105	Applications	Species
Huynh TN, Fikse EN, De La Torre AL et Al. Inhibiting the Cholesterol Storage Enzyme ACAT1/SOAT1 in Aging Apolipoprotein E4 Mice Alters Their Brains' Inflammatory Profiles Int J Mol Sci 2024-12-21 [PMID: 39769453]
Ren Sheng, Yining Li, Yexiang Wu, Chang Liu, Weizhi Wang, Xiaowan Han, Yinghong Li, Lijuan Lei, Xinhai Jiang, Yuyan Zhang, Yuhao Zhang, Shunwang Li, Bin Hong, Chao Liu, Yanni Xu, Shuyi Si A pan-PPAR agonist E17241 ameliorates hyperglycemia and diabetic dyslipidemia in KKAy mice via up-regulating ABCA1 in islet, liver, and white adipose tissue. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024-03-04 [PMID: 38308968]
Xia J, Wang W, Xia X et al. Triptolide suppresses secretion of inflammatory factors and promotes expression of ABCA1 International Journal of Clinical and Experimental Medicine 2019-06-30 (WB, Mouse)	WB	Mouse
Xue C, Zeng P, Gong K et Al. Nogo-B inhibition facilitates cholesterol metabolism to reduce hypercholesterolemia Cell Rep 2024-09-03 [PMID: 39235944]
Eva Tsaousidou, Jędrzej Chrzanowski, Pascal Drané, Grace Y Lee, Nadine Bahour, Zeqiu Branden Wang, Shijun Deng, Zhe Cao, Kaimeng Huang, Yizhou He, Mateusz Kaminski, Dominika Michalek, Ekin Güney, Kalindi Parmar, Wojciech Fendler, Dipanjan Chowdhury, Gökhan S Hotamışlıgil Endogenous p53 inhibitor TIRR dissociates systemic metabolic health from oncogenic activity. Cell reports 2024-07-01 [PMID: 38861384]
Fabiano M, Oikawa N, Kerksiek A, Furukawa JI et Al. Presenilin Deficiency Results in Cellular Cholesterol Accumulation by Impairment of Protein Glycosylation and NPC1 Function Int J Mol Sci 2024-05-25 [PMID: 38791456]
Juan Vladimir de la Rosa, Carlos Tabraue, Zhiqiang Huang, Marta C. Orizaola, Patricia Martin‐Rodríguez, Knut R. Steffensen, Juan Manuel Zapata, Lisardo Boscá, Peter Tontonoz, Susana Alemany, Eckardt Treuter, Antonio Castrillo Reprogramming of the LXR alpha Transcriptome Sustains Macrophage Secondary Inflammatory Responses Advanced Science 2024-03-28 [PMID: 38549193]
Stefanie Steinbauer, Alice König, Cathrina Neuhauser, Bettina Schwarzinger, Herbert Stangl, Marcus Iken, Julian Weghuber, Clemens Röhrl Elder ( Sambucus nigra ), identified by high-content screening, counteracts foam cell formation without promoting hepatic lipogenesis Scientific Reports 2024-02-12 [PMID: 38347122]
Carneiro de Oliveira K, Wei Y, Repetti RL, Meth J et Al. Tubular deficiency of ABCA1 augments cholesterol- and Na(+)-dependent effects on systemic blood pressure in male mice Am J Physiol Renal Physiol 2023-12-28 [PMID: 38153852]
Wiener JP, Desire S, Garliyev V et al. Down-Regulation of ABCA7 in Human Microglia, Astrocyte and THP-1 Cell Lines by Cholesterol Depletion, IL-1? and TNF?, or PMA Cells 2023-08-25 [PMID: 37681876]
Miyagawa S, Horie T, Nishino T et al. Inhibition of microRNA-33b in humanized mice ameliorates nonalcoholic steatohepatitis Life science alliance 2023-08-01 [PMID: 37263777] (WB, Mouse, Human)	WB	Mouse, Human
Dragoljevic D, Lee MKS, Pernes G et al. Administration of an LXR agonist promotes atherosclerotic lesion remodelling in murine inflammatory arthritis Clinical & translational immunology 2023-04-18 [PMID: 37091327] (IHC, Mouse) Details: Mouse lesions in arthritic mice	IHC	Mouse

Mao M, Deng Y, Wang L et al. Chronic unpredictable mild stress promotes atherosclerosis via adipose tissue dysfunction in ApoE(-/-) mice PeerJ 2023-09-04 [PMID: 37692113]
Resetar M, Tietcheu Galani BR, Tsamo AT et al. Flindissone, a Limonoid Isolated from Trichilia prieuriana, Is an LXR Agonist Journal of Natural Products 2023-08-25 [PMID: 37526502] (Western Blot, Block/Neutralize)	Western Blot, Block/Neutralize
Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 [PMID: 37432260] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence
Molina-Gonzalez I, Holloway RK, Jiwaji Z et al. Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration Nature communications 2023-06-08 [PMID: 37291151] (ICC/IF, Mouse)	ICC/IF	Mouse
Cao D, Khan Z, Li X et al. Macrophage angiotensin-converting enzyme (ACE) reduces atherosclerosis by increasing PPAR? and fundamentally changing lipid metabolism Cardiovascular research 2023-05-24 [PMID: 37225143] (WB, Mouse)	WB	Mouse
Cashikar A, Rios D, Timm D et al. Regulation of astrocyte lipid metabolism and ApoE secretion by the microglial oxysterol, 25-hydroxycholesterol Journal of lipid research 2023-02-25 [PMID: 36849076] (Mouse)		Mouse
Li Z, He M, Chen G et al. Effect of Total Sphingomyelin Synthase Activity on Low Density Lipoprotein Catabolism in Mice bioRxiv : the preprint server for biology 2023-02-07 [PMID: 36798262] (WB, Mouse)	WB	Mouse
Yalcinkaya M, Fotakis P, Liu W et al. Cholesterol accumulation in macrophages drives NETosis in atherosclerotic plaques via IL-1b secretion Cardiovascular research 2022-12-20 [PMID: 36537208] (WB, Mouse)	WB	Mouse
Wang H, Zheng A, Arias EB, Cartee GD. Prior AICAR induces elevated glucose uptake concomitant with greater ?3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats Facets (Ott) 2022-11-16 [PMID: 36381195]
Ma C, Zhang J, Yang S et al. Astragalus flavone ameliorates atherosclerosis and hepatic steatosis via improving lipid metabolism and inhibition of inflammation in apoE-/- mice Research Square Sep 3 2020
Dubland JA. Low lysosomal acid lipase activity in smooth muscle cells relative to macrophages provides new insights into foam cell formation in atherosclerosis. Thesis. 2018-01-01 (WB, Human)	WB	Human
Papotti B, Adorni MP, Marchi C et al. PCSK9 Affects Astrocyte Cholesterol Metabolism and Reduces Neuron Cholesterol Supplying In Vitro: Potential Implications in Alzheimer's Disease International journal of molecular sciences 2022-10-13 [PMID: 36293049] (WB, Human)	WB	Human

Cassim Bawa FN, Gopoju R, Xu Y et al. Retinoic Acid Receptor Alpha (RARa) in Macrophages Protects from Diet-Induced Atherosclerosis in Mice Cells 2022-10-11 [PMID: 36291054] (WB, Mouse)	WB	Mouse
Cai Y, Wang Z, Li L et al. Neuropeptide Y regulates cholesterol uptake and efflux in macrophages and promotes foam cell formation Journal of cellular and molecular medicine 2022-09-29 [PMID: 36172879] (WB, Human)	WB	Human
Man B, Hu C, Yang G et al. Berberine attenuates diabetic atherosclerosis via enhancing the interplay between KLF16 and PPAR alpha in ApoE-/- mice Biochemical and Biophysical Research Communications 2022-07-01 [PMID: 35933927] (WB, Mouse) Details: Figure 3E	WB	Mouse
Wang JQ, Li LL, Hu A Et al. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion Nature 2022-08-03 [PMID: 35922515] (WB) Details: Citation using the FITC version of this antibody.	WB
Perelroizen R, Philosof B, Budick-Harmelin N et al. Astrocyte immunometabolic regulation of the tumour microenvironment drives glioblastoma pathogenicity Brain : a journal of neurology 2022-07-28 [PMID: 35899587] (IHC-Fr, WB, Mouse)	IHC-Fr, WB	Mouse
Vaisar T, Wang S, Omer M et al. 10,12-conjugated linoleic acid supplementation improves HDL composition and function in mice Journal of lipid research 2022-06-14 [PMID: 35714730] (WB, Mouse)	WB	Mouse
My-Anh Nguyen, Huy-Dung Hoang, Adil Rasheed, Anne-Claire Duchez, Hailey Wyatt, Mary Lynn Cottee, Tyson E. Graber, Leah Susser, Sabrina Robichaud, İbrahim Berber, Michele Geoffrion, Mireille Ouimet, Hilal Kazan, Lars Maegdefessel, Erin E. Mulvihill, Tommy Alain, Katey J. Rayner miR-223 Exerts Translational Control of Proatherogenic Genes in Macrophages Circulation Research 2022-05-25 [PMID: 35611698]
Wang W, Lokman N, Noye T et al. ABCA1 is associated with the development of acquired chemotherapy resistance and predicts poor ovarian cancer outcome Cancer Drug Resist 2022-05-18 [PMID: 35582032]
Li J, Zhu Z, Li Y et al. D-4F, an apolipoprotein A-I mimetic, promotes the clearance of myelin debris and the reduction of foamy macrophages after spinal cord injury Bioengineered 2022-05-12 [PMID: 35546071]
Picataggi A, Rodrigues A, Cromley DA et al. Specificity of ABCA7-mediated cell lipid efflux Biochimica et biophysica acta. Molecular and cell biology of lipids 2022-07-01 [PMID: 35381375] (WB, Human)	WB	Human
Yano H, Fujiwara Y, Horlad H et al. Blocking cholesterol efflux mechanism is a potential target for antilymphoma therapy Cancer science 2022-06-01 [PMID: 35343027] (WB, Human)	WB	Human
Peters F, Ebner LJA, Atac D et al. Regulation of ABCA1 by AMD-Associated Genetic Variants and Hypoxia in iPSC-RPE International journal of molecular sciences 2022-03-16 [PMID: 35328615] (WB, Human)	WB	Human
Yildirim Z, Baboo S, Hamid SM et al. Intercepting IRE1 kinase-FMRP signaling prevents atherosclerosis progression EMBO molecular medicine 2022-02-22 [PMID: 35191199] (WB, Mouse)	WB	Mouse
Balamurugan K, Medishetti R, Kotha J et al. PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways iScience 2022-02-01 [PMID: 35141506] (WB, Human, Mouse)	WB	Human, Mouse
Toma L, Barbalata T, Sanda G Et al. CRISPR/dCas9 Transcriptional Activation of Endogenous Apolipoprotein AI and Paraoxonase 1 in Enterocytes Alleviates Endothelial Cell Dysfunction Biomolecules 2021-11-25 [PMID: 34944413] (WB, Human)	WB	Human
Kane J, Jansen M, Hendrix S et al. Anti-Galectin-2 antibody treatment reduces atherosclerotic plaque size and alters macrophage polarity Thrombosis and haemostasis 2021-12-01 [PMID: 34852377] (IF/IHC, Mouse)	IF/IHC	Mouse
Ma C, Wu H, Yang G, Xiang J et Al. Calycosin ameliorates atherosclerosis by enhancing autophagy via regulating the interaction between KLF2 and MLKL in apolipoprotein E gene-deleted mice Br J Pharmacol 2021-10-29 [PMID: 34713437]
Huuska N, Netti E, Tulamo R et al. Serum Amyloid A Is Present in Human Saccular Intracranial Aneurysm Walls and Associates With Aneurysm Rupture Journal of Neuropathology & Experimental Neurology 2021-10-01 [PMID: 34534311] (Immunohistochemistry)	Immunohistochemistry
Roberts LM, Schwarz B, Speranza E et al. Pulmonary infection induces persistent, pathogen-specific lipidomic changes influencing trained immunity iScience 2021-09-24 [PMID: 34522865]
Casado ME, Huerta L, Marcos-DIaz A Et al. Hormone-sensitive lipase deficiency affects the expression of SR-BI, LDLr, and ABCA1 receptors/transporters involved in cellular cholesterol uptake and efflux and disturbs fertility in mouse testis Biochimica et biophysica acta. Molecular and cell biology of lipids 2021-08-27 [PMID: 34461308] (IF/IHC, Mouse)	IF/IHC	Mouse
Han Y, Onufer E, Huang L et al. Enterically derived high-density lipoprotein restrains liver injury through the portal vein Science 2021-07-23 [PMID: 34437091]
Huang R, Hu Z, Chen X Et al. The Transcription Factor SUB1 Is a Master Regulator of the Macrophage TLR Response in Atherosclerosis Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021-08-10 [PMID: 34378353]
Wright MB, Varona Santos J, Kemmer C Et al. Compounds targeting OSBPL7 increase ABCA1-dependent cholesterol efflux preserving kidney function in two models of kidney disease Nature communications 2021-08-02 [PMID: 34341345] (IF/IHC, Mouse)	IF/IHC	Mouse
Mota AC, Dominguez M, Weigert A et al. Lysosome-Dependent LXR and PPAR delta Activation Upon Efferocytosis in Human Macrophages Frontiers in immunology 2021-05-07 [PMID: 34025647] (WB, Human)	WB	Human
Zhang S, Li Z, Zhang Y et al. Ketone Body 3 Hydroxybutyrate Ameliorates Atherosclerosis via Receptor Gpr109a Mediated Calcium Influx Advanced Science 2021-03-01 [PMID: 33977048] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Patel S THE ROLE OF MYELOID GSK3a/B IN ATHEROSCLEROSIS AND ATHEROSCLEROTIC REGRESSION Thesis 2022-01-01 (IHC-P, Mouse)	IHC-P	Mouse
Navia-Pelaez JM, Choi SH, Dos Santos Aggum Capettini L et al. Normalization of cholesterol metabolism in spinal microglia alleviates neuropathic pain The Journal of experimental medicine 2021-07-05 [PMID: 33970188]
Wang H, Arias EB, Cartee GD Reduced Membrane Cholesterol Content in Skeletal Muscle Is Not Essential for Greater Insulin-stimulated Glucose Uptake after Acute Exercise by Rats Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 2021-03-25 [PMID: 33765397]

Brampton C, Pomozi V, Chen LH et al. ABCC6 deficiency promotes dyslipidemia and atherosclerosis Scientific reports 2021-02-16 [PMID: 33594095] (ICC/IF, Mouse)	ICC/IF	Mouse
Horie T, Nakao T, Miyasaka Y et al. microRNA-33 maintains adaptive thermogenesis via enhanced sympathetic nerve activity Nature communications 2021-02-16 [PMID: 33594062] (WB, Mouse)	WB	Mouse
Alajbegovic A, Holmberg J, Daoud F, et al. MRTFA overexpression promotes conversion of human coronary artery smooth muscle cells into lipid-laden foam cells Vascular pharmacology 2021-01-28 [PMID: 33516965] (WB, Human)	WB	Human
Ma C, Feng K, Yang X, et al. Targeting macrophage liver X receptor by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis British journal of pharmacology 2021-01-28 [PMID: 33506494]
Lv JJ, Wang H, Cui HY, et al. Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis Frontiers in cell and developmental biology 2020-01-08 [PMID: 33490072] (ICC/IF, WB, Mouse)	ICC/IF, WB	Mouse
Palafox-Sanchez V, Ying Z, Royes LFF, Gomez-Pinilla F The interaction between brain and liver regulates lipid metabolism in the TBI pathology Biochimica et biophysica acta. Molecular basis of disease 2021-01-12 [PMID: 33444711] (WB, Rat)	WB	Rat
Xu (???) Y, Liu (??) C, Han (???) X et al. E17241 as a Novel ABCA1 (ATP-Binding Cassette Transporter A1) Upregulator Ameliorates Atherosclerosis in Mice Arteriosclerosis, Thrombosis, and Vascular Biology 2021-06-01 [PMID: 33441025]
Ma C, Zhang J, Yang S et al. Astragalus Flavone Ameliorates Atherosclerosis and Hepatic Steatosis Via Inhibiting Lipid-Disorder and Inflammation in apoE-/- Mice Frontiers in Pharmacology 2020-12-14 [PMID: 33381046] (WB, Mouse)	WB	Mouse
Ren Q, Xie X, Tang Y et al. Methyl tertiary-butyl ether inhibits THP-1 macrophage cholesterol efflux in vitro and accelerates atherosclerosis in ApoE-deficient mice in vivo J Environ Sci (China) 2020-12-18 [PMID: 33334519]
Fu Y, Sun Y, Wang M et al. Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism Cell Metab 2020-12-01 [PMID: 33186558]
Mukherjee V, Ramakrishna P, Bora S, Kotteazeth S Phytosteroid 28-homobrassinolide targets cholesterol and glucose homeostasis implicating ABCA1 and SREBP role in regulation Steroids 2020-11-07 [PMID: 33171131] (WB, Human) Details: Western blot analysis performed on HepG2 cells.	WB	Human
Dodge JC, Jensen EH, Yu J et al. Neutral lipid cacostasis contributes to disease pathogenesis in amyotrophic lateral sclerosis J Neurosci 2020-10-13 [PMID: 33051352]
Munir MT, Ponce C, Santos JM et al. VD3 and LXR agonist (T0901317) combination demonstrated greater potency in inhibiting cholesterol accumulation and inducing apoptosis via ABCA1-CHOP-BCL-2 cascade in MCF-7 breast cancer cells Mol Biol Rep 2020-10-01 [PMID: 32990902]
Zhao D, Li J, Xue C et al. TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells J. Biol. Chem. 2020-09-22 [PMID: 32963108] (Mouse)		Mouse
Shang Y, Ma C, Zhang J et al. Bifunctional supramolecular nanofiber inhibits atherosclerosis by enhancing plaque stability and anti-inflammation in apoE-/- mice Theranostics 2020-08-13 [PMID: 32929345] (WB, Mouse)	WB	Mouse
Soo-Ho Choi, Keun-Young Kim, Guy A. Perkins, Sébastien Phan, Genea Edwards, Yining Xia, Jungsu Kim, Dorota Skowronska-Krawczyk, Robert N. Weinreb, Mark H. Ellisman, Yury I. Miller, Won-Kyu Ju AIBP protects retinal ganglion cells against neuroinflammation and mitochondrial dysfunction in glaucomatous neurodegeneration Redox Biology 2020-08-27 [PMID: 32896719]
Venkat P, Zacharek A, Landschoot-Ward J et al. Exosomes derived from bone marrow mesenchymal stem cells harvested from type two diabetes rats promotes neurorestorative effects after stroke in type two diabetes rats Experimental neurology 2020-09-02 [PMID: 32889008] (IHC-P, Rat)	IHC-P	Rat
Xia X, Xu Q, Liu M et al. Deubiquitination of CD36 by UCHL1 promotes foam cell formation Cell Death Dis 2020-08-15 [PMID: 32801299] (IP)	IP
Gao Q, Wei A, Chen F et al. Enhancing PPAR gamma by HDAC inhibition reduces foam cell formation and atherosclerosis in ApoE deficient mice Pharmacological Research 2020-07-01 [PMID: 32621955] (WB, Mouse)	WB	Mouse
Werkman IL, KOvilein J, de Jonge JC, Baron W Impairing committed cholesterol biosynthesis in white matter astrocytes, but not grey matter astrocytes, enhances in vitro myelination J. Neurochem. 2020-06-30 [PMID: 32602556] (WB, Rat)	WB	Rat
Wang D, Hiebl V, Schachner D et al. Soraphen A enhances macrophage cholesterol efflux via indirect LXR activation and ABCA1 upregulation Biochem. Pharmacol. 2020-05-08 [PMID: 32437644] (WB, Human)	WB	Human
Marshall J, Courage E, Elliott R et al. THP-1 Macrophage Cholesterol Efflux Is Impaired by Palmitoleate Through Akt Activation PLoS One. 2020-05-21 [PMID: 32437392] (WB, Human)	WB	Human
Pathak P, Helsley RN, Brown AL et al. Small Molecule Inhibition of Gut Microbial Choline Trimethylamine Lyase Activity Alters Host Cholesterol and Bile Acid Metabolism Am. J. Physiol. Heart Circ. Physiol. 2020-04-24 [PMID: 32330092] (WB, Mouse)	WB	Mouse
Hiebl V, Schachner D, Ladurner A et al. Caco-2 Cells for Measuring Intestinal Cholesterol Transport - Possibilities and Limitations Biol Proced Online 2020-04-11 [PMID: 32308567]
Salonurmi T, Nabil H, Ronkainen J et Al. 4 beta-Hydroxycholesterol Signals From the Liver to Regulate Peripheral Cholesterol Transporters Front. Pharmacol. 2020-03-27 [PMID: 32292343] (WB, Rat)	WB	Rat
He Q, Wang F, Honda T et al. Ablation of miR-144 increases vimentin expression and atherosclerotic plaque formation Sci Rep 2020-04-09 [PMID: 32273567] (KO, Mouse)	KO	Mouse
B Fuenzalida, C Cantin, S Kallol, L Carvajal, V Pastén, S Contreras-, C Albrecht, J Gutierrez, A Leiva Cholesterol uptake and efflux are impaired in human trophoblast cells from pregnancies with maternal supraphysiological hypercholesterolemia Sci Rep, 2020-03-24;10(1):5264. 2020-03-24 [PMID: 32210256]
Koga M, Kanaoka Y, Inada K et al. Hesperidin blocks varenicline-aggravated atherosclerotic plaque formation in apolipoprotein E knockout mice by downregulating net uptake of oxidized low-density lipoprotein in macrophages J. Pharmacol. Sci. 2020-02-28 [PMID: 32173266] (WB, Mouse)	WB	Mouse
Bogie Jfj, Grajchen E, Wouters E et Al. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain J. Exp. Med. 2020-05-04 [PMID: 32097464] (FLOW, Mouse)	FLOW	Mouse
Cipollari, E, Szapary, H J Et al. Correlates and Predictors of Cerebrospinal Fluid Cholesterol Efflux Capacity from Neural Cells, a Family of Biomarkers for Cholesterol Epidemiology in Alzheimer's Disease. J Alzheimers Dis 2020-02-18 [PMID: 32065798] (WB, Rat)	WB	Rat
Matsuda J, Takahashi A, Takabatake Y et al. Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells Autophagy 2020-01-16 [PMID: 31944172]
Chen Y, Guo KM, Nagy T, Guo TL Metabolomics study of the prefrontal cortex in a rat model of attention deficit hyperactivity disorder reveals the association between cholesterol metabolism disorder and hyperactive behavior Biochem. Biophys. Res. Commun. 2019-12-18 [PMID: 31864712] (WB, Rat)	WB	Rat
Koga M, Kanaoka Y, Okamoto M et al. Varenicline aggravates atherosclerotic plaque formation in nicotine-pretreated ApoE knockout mice due to enhanced oxLDL uptake by macrophages through downregulation of ABCA1 and ABCG1 expression J. Pharmacol. Sci. 2019-11-14 [PMID: 31771811] (WB, Mouse)	WB	Mouse
Du Y, Li X, Su C et al. Butyrate protects against high-fat diet-induced atherosclerosis via up-regulating ABCA1 expression in apolipoprotein E-deficiency mice Br. J. Pharmacol. 2019-11-25 [PMID: 31769014]
Pagano S, Magenta A, D'Agostino M et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation J Clin Med 2019-11-21 [PMID: 31766415] (WB, Human)	WB	Human
Kaymak I, Maier CR, Schmitz W et al. Mevalonate pathway provides ubiquinone to maintain pyrimidine synthesis and survival in p53-deficient cancer cells exposed to metabolic stress Cancer Res. 2019-11-19 [PMID: 31744820] (WB, Mouse, Human)	WB	Mouse, Human
Xiao L, Luo G, Guo X et al. Macrophage iron retention aggravates atherosclerosis: Evidence for the role of autocrine formation of hepcidin in plaque macrophages Biochim Biophys Acta Mol Cell Biol Lipids 2019-10-27 [PMID: 31666189] (KD, WB, ICC/IF, Human, Mouse)	KD, WB, ICC/IF	Human, Mouse
Smith TKT, Kahiel Z, LeBlond ND et al. Characterization of Redox-Responsive LXR-Activating Nanoparticle Formulations in Primary Mouse Macrophages Molecules 2019-10-18 [PMID: 31635211] (WB, Mouse)	WB	Mouse
Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019-09-12 [PMID: 31604677]
Hennessy EJ, van Solingen C, Scacalossi KR et al. The long noncoding RNA CHROME regulates cholesterol homeostasis in primate Nat Metab 2019-01-01 [PMID: 31410392] (WB, Human)	WB	Human
Zhou Y, Bazick H, Miles JR et al. A neutral lipid-enriched diet improves myelination and alleviates peripheral nerve pathology in neuropathic mice Exp. Neurol. 2019-08-03 [PMID: 31386828] (WB, IHC-Fr, Mouse)	WB, IHC-Fr	Mouse
Paul A, Lydic TA, Hogan R, Goo YH Cholesterol Acceptors Regulate the Lipidome of Macrophage Foam Cells Int J Mol Sci 2019-08-02 [PMID: 31382484] (WB, Mouse)	WB	Mouse
Mukhamedova N, Hoang A, Dragoljevic D et al. Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells PLoS Pathog. 2019-07-01 [PMID: 31344124] (ICC/IF, Human, Mouse)	ICC/IF	Human, Mouse
Koyama S, Horie T, Nishino T et al. Identification of Differential Roles of MicroRNA-33a and -33b During Atherosclerosis Progression With Genetically Modified Mice J Am Heart Assoc 2019-07-02 [PMID: 31242815] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Rege SD, Luiz R, Tsai B et al. Brain Trauma Disrupts Hepatic Lipid Metabolism: Blame it on Fructose? Mol Nutr Food Res 2019-05-13 [PMID: 31087499] (WB, Rat)	WB	Rat
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Ni S, Ma L, Denis M et al. ABCA1-mediated cholesterol efflux generates microparticles in addition to HDL through processes governed by membrane rigidity. J Lipid Res;50(3):456-66. 2009-03-01 [PMID: 18941142] (ICC/IF, Hamster)	ICC/IF	Hamster
Pou J, Rebollo A, Roglans N, Sanchez RM, Vazquez-Carrera M, Laguna JC, Pedro-Botet J, Alegret M. Ritonavir increases CD36, ABCA1 and CYP27 expression in THP-1 macrophages. Exp Biol Med (Maywood);233(12):1572-82. 2008-12-01 [PMID: 18849545]
Robichaud JC, Francis GA, Vance DE. A role for hepatic scavenger receptor class B, type I in decreasing high density lipoprotein levels in mice that lack phosphatidylethanolamine N-methyltransferase. J Biol Chem 2008-12-19 [PMID: 18842588]
Delvecchio CJ, Bilan P, Nair P et al. LXR-induced reverse cholesterol transport in human airway smooth muscle is mediated exclusively by ABCA1. Am J Physiol Lung Cell Mol Physiol;295(5):L949-57. 2008-11-01 [PMID: 18820007]
Nakanishi S, Vikstedt R, Soderlund S et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity. J Lipid Res;50(2):183-92. 2009-02-01 [PMID: 18787236]
Cartagena CM, Ahmed F, Burns MP, Pajoohesh-Ganji A, Pak DT, Faden AI, Rebeck GW. Cortical injury increases cholesterol 24S hydroxylase (Cyp46) levels in the rat brain. J Neurotrauma;25(9):1087-98. 2008-09-01 [PMID: 18729719]
Peng D, Hiipakka RA, Dai Q, Guo J, Reardon CA, Getz GS, Liao S. Antiatherosclerotic effects of a novel synthetic tissue-selective steroidal liver X receptor agonist in low-density lipoprotein receptor-deficient mice. J Pharmacol Exp Ther;327(2):332-42. 2008-11-01 [PMID: 18723776]
Mukhamedova N, Escher G, D'Souza W, Tchoua U, Grant A, Krozowski Z, Bukrinsky M, Sviridov D. Enhancing apolipoprotein A-I-dependent cholesterol efflux elevates cholesterol export from macrophages in vivo. J Lipid Res;49(11):2312-22. 2008-11-01 [PMID: 18622028] (WB, IP, Mouse)	WB, IP	Mouse
Paul V, Meyer HH, Leidl K, Soumian S, Albrecht C. A novel enzyme immunoassay specific for ABCA1 protein quantification in human tissues and cells. J Lipid Res;49(10):2259-67. 2008-10-01 [PMID: 18541924] (WB, ELISA, Human)	WB, ELISA	Human
Frank PG, Pavlides S, Cheung MW, Daumer K, Lisanti MP. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol;295(1):C242-8. 2008-07-01 [PMID: 18508910]
Kumar R, McClain D, Young R et al. Cholesterol transporter ATP-binding cassette A1 (ABCA1) is elevated in prion disease affects PrPC PrPSc concentrations in cultured cells. J Gen Virol;89(6):1525-1532. 2008-01-01 [PMID: 18474570]
Bowden K, Ridgway ND. OSBP negatively regulates ABCA1 protein stability. J Biol Chem;283(26):18210-7. 2008-06-27 [PMID: 18450749] (WB, Mouse)	WB	Mouse
Tancevski I, Wehinger A, Demetz E, Eller P, Duwensee K, Huber J, Hochegger K, Schgoer W, Fievet C, Stellaard F, Rudling M, Patsch JR, Ritsch A. Reduced plasma high-density lipoprotein cholesterol in hyperthyroid mice coincides with decreased hepatic adenosine 5'-triphosphate-binding cassette transporter 1 expression. Endocrinology;149(7):3708-12. 2008-07-01 [PMID: 18388200]
Ouimet M, Wang M-D, Cadotte N et al. Epoxycholesterol Impairs Cholesteryl Ester Hydrolysis in Macrophage Foam Cells, Resulting in Decreased Cholesterol Efflux. Arterioscler Thromb Vasc Biol;28(6):1144-1150. 2008-01-01 [PMID: 18369155] (WB)	WB
Rode S, Rubic T, Lorenz RL et al. alpha-Tocopherol disturbs macrophage LXRalpha regulation of ABCA1/G1 and cholesterol handling. Biochem Biophys Res Commun 2008-05-09 [PMID: 18328805] (WB, Human)	WB	Human
Smoak K, Madenspacher J, Jeyaseelan S, Williams B, Dixon D, Poch KR, Nick JA, Worthen GS, Fessler MB. Effects of liver X receptor agonist treatment on pulmonary inflammation and host defense. J Immunol;180(5):3305-12. 2008-03-01 [PMID: 18292555]
Henry-Berger J, Mouzat K, Baron S, Bernabeu C, Marceau G, Saru JP, Sapin V, Lobaccaro JM, Caira F. Endoglin (CD105) expression is regulated by the liver X receptor alpha (NR1H3) in human trophoblast cell line JAR. Biol Reprod;78(6):968-75. 2008-06-01 [PMID: 18276933]
Montoudis A, Seidman E, Boudreau F et al. Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake. J Lipid Res;49(5):961-72. 2008-05-01 [PMID: 18235139] (WB)	WB
Watanabe T, Nishio K, Kanome T, Matsuyama TA, Koba S, Sakai T, Sato K, Hongob S, Nose K, Ota H, Kobayashi Y, Katagiri T, Shichiri M, Miyazaki A. Impact of salusin-alpha and -beta on human macrophage foam cell formation and coronary atherosclerosis. Circulation;117(5):638-48. 2008-02-05 [PMID: 18212280]
Hastie C, Masters JR, Moss SE, Naaby-Hansen S. Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells. J Biol Chem;283(18):12595-603. 2008-05-02 [PMID: 18211896]
Zhu M, Fu Y, Hou Y et al. Laminar Shear Stress Regulates Liver X Receptor in Vascular Endothelial Cells. Arterioscler Thromb Vasc Biol;28(3):527-33. 2008-03-01 [PMID: 18096827] (WB)	WB
Jacobs RL, Lingrell S, Zhao Y, Francis GA, Vance DE. Hepatic CTP:phosphocholine cytidylyltransferase-alpha is a critical predictor of plasma high density lipoprotein and very low density lipoprotein. J Biol Chem;283(4):2147-55. 2008-01-25 [PMID: 18042552]
Wang N, Yvan-Charvet L, Lutjohann D, Mulder M, Vanmierlo T, Kim TW, Tall AR. ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain. FASEB J;22(4):1073-82. 2008-04-01 [PMID: 18039927]
Zhou X et al. Genetic deletion of low density lipoprotein receptor impairs sterol-induced mouse macrophage ABCA1 expression. A new SREBP1-dependent mechanism. J Biol Chem. 283(4):2129-38. 2008-01-25 [PMID: 18029360]
Yan D, Mayranpaa MI, Wong J, Perttila J, Lehto M, Jauhiainen M, Kovanen PT, Ehnholm C, Brown AJ, Olkkonen VM. OSBP-related protein 8 (ORP8) suppresses ABCA1 expression and cholesterol efflux from macrophages. J Biol Chem;283(1):332-40. 2008-01-04 [PMID: 17991739]
Out, R et al. Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporters A1 and G1. Circ Res;102(1):113-20. 2008-01-04 [PMID: 17967783]
Hoe, H et al. The Metalloprotease Inhibitor TIMP-3 Regulates Amyloid Precursor Protein Apolipoprotein E Receptor Proteolysis. J Neurosci27: 10895-10905. 2007-10-01 [PMID: 17913923]
Bates SR, Tao JQ, Yu KJ, Borok Z, Crandall ED, Collins HL, Rothblat GH. Expression and biological activity of ABCA1 in alveolar epithelial cells. Am J Respir Cell Mol Biol;38(3):283-92. 2008-03-01 [PMID: 17884990] (IF/IHC, Rat)	IF/IHC	Rat
Talukdar S, Bhatnagar S, Dridi S, Hillgartner FB. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist T0-901317. J Lipid Res;48(12):2647-63. 2007-12-01 [PMID: 17823458] (WB, Chicken)	WB	Chicken
Babaev VR, Ishiguro H, Ding L, Yancey PG, Dove DE, Kovacs WJ, Semenkovich CF, Fazio S, Linton MF. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation;116(12):1404-12. 2007-09-18 [PMID: 17724261]
Hassan HH, Denis M, Lee DY, Iatan I, Nyholt D, Ruel I, Krimbou L, Genest J. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-I binding site for nascent HDL formation: implications for current models of HDL biogenesis. J Lipid Res;48(11):2428-42. 2007-11-01 [PMID: 17656736]
During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res;48(10):2283-94. 2007-10-01 [PMID: 17644776]
Atshaves BP, McIntosh AL, Payne HR, Gallegos AM, Landrock K, Maeda N, Kier AB, Schroeder F. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res;48(10):2193-211. 2007-10-01 [PMID: 17609524]
Zelcer N, Khanlou N, Clare R et al. Attenuation of neuroinflammation and Alzheimer's disease pathology by liver x receptors. Proc Natl Acad Sci U S A 2007-06-19 [PMID: 17563384] (Mouse)		Mouse
Sporstol M, Mousavi SA, Eskild W et al. ABCA1, ABCG1 and SR-BI: hormonal regulation in primary rat hepatocytes and human cell lines. BMC Mol Biol;8:5. 2007-01-22 [PMID: 17241464]
Zhang, C et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J Lipid Res. 2006-01-01 [PMID: 16807312] (WB, Porcine)	WB	Porcine
Trogan, E et al. Gene expression changes in foam cells the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS 103:3781-3786. 2006-01-01 [PMID: 16537455] (IF/IHC, Mouse)	IF/IHC	Mouse
Dong J, Liu J, Lou B et al. Adenovirus-mediated overexpression of sphingomyelin synthases 1 and 2 increases the atherogenic potential in mice. J Lipid Res;47(6):1307-14. 2006-06-01 [PMID: 16508036]
Gerbod-Giannone, M-C et al. TNFa induces ABCA1 through NF-kappaB in macrophage in phagocytes ingesting apoptotic cells. PNAS 103:3112-3117. 2006-01-01 [PMID: 16492740]
Duong PT et al. Characterization of nascent HDL particles microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res 47:832-843. 2006-01-01 [PMID: 16418537]
Boisvert WA, Rose DM, Boullier A, Quehenberger O, Sydlaske A, Johnson KA, Curtiss LK, Terkeltaub R. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size. Arterioscler Thromb Vasc Biol;26(3):563-9. 2006-03-01 [PMID: 16410462]
Takata Y, Chu V, Collins AR et al. Transcriptional repression of ATP-binding cassette transporter A1 gene in macrophages: a novel atherosclerotic effect of angiotensin II. Circ Res 2005-10-28 [PMID: 16224068] (WB, Mouse)	WB	Mouse
Berfield, AK et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am J Physiol Renal Physiol 290:138-147. 2006-01-01 [PMID: 16077083]
Forcheron F, Legedz L, Chinetti G, Feugier P, Letexier D, Bricca G, Beylot M. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. Arterioscler Thromb Vasc Biol;25(8):1711-7. 2005-08-01 [PMID: 15961705] (WB, Human, Rat)	WB	Human, Rat
Glaros, EN et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J Biol Chem 280(26): 24515-24523. 2005-01-01 [PMID: 15890646] (WB, Human)	WB	Human
Ragozin S, Niemeier A, Laatsch A et al. Knockdown of hepatic ABCA1 by RNA interference decreases plasma HDL cholesterol levels and influences postprandial lipemia in mice. Arterioscler Thromb Vasc Biol 2005-07-01 [PMID: 15845910]
Makowski L, Brittingham KC, Reynolds JM et al. The fatty acid-binding protein, aP2, coordinates macrophage cholesterol trafficking and inflammatory activity. Macrophage expression of aP2 impacts peroxisome proliferator-activated receptor gamma and IkappaB kinase activities. J Biol Chem 2005-04-01 [PMID: 15684432] (EMSA, Mouse)	EMSA	Mouse
Li A et al. Differential inhibition of macrophage foam-cell formation atherosclerosis in mice by PPAR alpha alpha, B/delta, gamma. J Clin Invest 114: 1564-1576. 2004-01-01 [PMID: 15578089]
Koldamova, R et al. The Liver Receptor Lig T0901317 Decreases Amyloid Beta Production in Vitro in a Mouse Model of Alzheimer';s Disease. The Journal of Biological Chemistry 280(6): 4079-4088. 2005-01-01 [PMID: 15557325] (WB, Mouse)	WB	Mouse
Dove DE, Su YR, Zhang W, Jerome WG, Swift LL, Linton MF, Fazio S. ACAT1 deficiency disrupts cholesterol efflux and alters cellular morphology in macrophages. Arterioscler Thromb Vasc Biol;25(1):128-34. 2005-01-01 [PMID: 15499044]
O'Connell BJ, Denis M, Genest J. Cellular physiology of cholesterol efflux in vascular endothelial cells. Circulation;110(18):2881-8. 2004-11-02 [PMID: 15492319]
Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol Biol Cell;15(12):5399-407. 2004-12-01 [PMID: 15469992]
Zeng, L et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J Biol Chem 279: 48801-48807. 2004-01-01 [PMID: 15358760] (WB, Human)	WB	Human
Denis, M et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry 279(40):41529-41536. 2004-01-01 [PMID: 15280376] (IP, Human)	IP	Human
Ando H, Tsuruoka S, Yamamoto H, Takamura T, Kaneko S, Fujimura A. Effects of pravastatin on the expression of ATP-binding cassette transporter A1. Exp Ther. 311(1):420-5. 2004-10-01 [PMID: 15210833]
Brown III, J et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer's Disease. The Journal of Biological Chemistry 279(33): 34674-34681. 2004-01-01 [PMID: 15148325] (WB, Human)	WB	Human
Agassandian M, Mathur SN, Zhou J, Field FJ, Mallampalli RK. Oxysterols trigger ABCA1-mediated basolateral surfactant efflux. Am J Respir Cell Mol Biol;31(2):227-33. 2004-08-01 [PMID: 15039140]
Sahoo, D et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research 45: 1122-1131. 2004-01-01 [PMID: 14993246] (WB, Rat)	WB	Rat
Field, F et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research 45:905-913. 2004-01-01 [PMID: 14993242] (WB, Human)	WB	Human
Krimbou L, Denis M, Haidar B et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research 45:839-848. 2004-01-01 [PMID: 14754908] (IP, Human)	IP	Human
Gaus K, Kritharides L, Schmitz G, Boettcher A, Drobnik W, Langmann T, Quinn CM, Death A, Dean RT, Jessup W. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. FASEB J;18(3):574-6. 2004-03-01 [PMID: 14734645]
Haidar, B et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J Biol Chem 279: 9963-9969. 2004-01-01 [PMID: 14701824] (IP, Human)	IP	Human
Gaus, K et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS 100(26): 15554-15559. 2003-01-01 [PMID: 14673117] (ICC/IF, Mouse, Human)	ICC/IF	Mouse, Human
Denis, M et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry 279(9):7384-7394. 2004-01-01 [PMID: 14660648] (IP, Human)	IP	Human
Su, Y et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice Up-regulates ABC Transporters. Molecular Therapy 0:1-8. 2003-01-01 [PMID: 14529830] (WB, Mouse)	WB	Mouse
Zager RA, Johnson AC, Hanson SY. Proximal tubular cholesterol loading after mitochondrial, but not glycolytic, blockade. Am J Physiol Renal Physiol 2003-12-01 [PMID: 12952856]
Bortnick, A. Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878. 2003-01-01 [PMID: 12909583] (WB, Human)	WB	Human
Wagner, B. Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, pp 5780-5789. 2003-08-01 [PMID: 12897148] (WB, Mouse)	WB	Mouse
Witting, S et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry 278(41):40121-40127. 2003-01-01 [PMID: 12890677] (WB, Mouse)	WB	Mouse
Khovidhunkit, W et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research 44: 1728-1736. 2003-01-01 [PMID: 12777468] (WB, Mouse)	WB	Mouse
Le Lay, S et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res 44(8): p 1499-1507. 2003-01-01 [PMID: 12754274] (WB, Mouse)	WB	Mouse
Sun, Y. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694. 2003-01-01 [PMID: 12754201] (WB, Mouse)	WB	Mouse
Terasaka, N et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters 536: 6-11. 2003-01-01 [PMID: 12586329] (IHC-P, Mouse)	IHC-P	Mouse
Basso, F et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research 44:296-302. 2003-01-01 [PMID: 12576511] (WB, Mouse)	WB	Mouse
Koldamova, R et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry 278(15):13244-13256. 2003-01-01 [PMID: 12547833] (WB, IF/IHC, ICC/IF, Rat)	WB, IF/IHC, ICC/IF	Rat
Escher, G et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry 278(13):11015-11019. 2003-01-01 [PMID: 12531903] (WB, Hamster, Human)	WB	Hamster, Human
Wang, N et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation111(1): 99-107. 2003-01-01 [PMID: 12511593] (WB, IP, Human)	WB, IP	Human
Johnson AC, Yabu JM, Hanson S et al. Experimental glomerulopathy alters renal cortical cholesterol, SR-B1, ABCA1, and HMG CoA reductase expression Am J Pathol. 2003-01-01 [PMID: 12507911] (WB, Mouse)	WB	Mouse
Haidar, B et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research 43(12):2087-2094. 2002-01-01 [PMID: 12454270] (IP, Human)	IP	Human
Reddy ST, Hama S, Ng C, Grijalva V, Navab M, Fogelman AM. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells. Arterioscler Thromb Vasc Biol;22(11):1877-83. 2002-11-01 [PMID: 12426219]
Fukumoto, H et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry 277(50):48508-48513. 2002-01-01 [PMID: 12384498] (WB, Human, Mouse)	WB	Human, Mouse
Feng, B et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry 277(45):43271-73280. 2002-01-01 [PMID: 12215451] (WB, Mouse)	WB	Mouse
Murthy, S et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J Lipid Research 43: 1054-1064. 2002-01-01 [PMID: 12091489] (WB, Human)	WB	Human
Burgess, JW et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press 277(35): 31318-31326. 2002-01-01 [PMID: 12050168] (WB, Human, Mouse)	WB	Human, Mouse
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Reviews for ABCA1 Antibody (NB400-105) (7) 4.37

Average Rating: 4.3

(Based on 7 reviews)

We have 7 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB400-105:

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reviewed by:
Verified Customer

Human

08/15/2021

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Summary

Application	Western Blot
Sample Tested	Human primary fibroblast
Species	Human
Lot	AM-5

Comments

10 ug of total protein. 7,5% TGX gel. Blocking 5% milk in PBST 1h RT. 1:1000 primary ab in BSA 3% PBST O/N at 4ºC. Secondary 1:5000 HRP 1h RT.

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reviewed by:
Jahangir Iqbal

Mouse

11/16/2015

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Summary

Application	Western Blot
Sample Tested	mouse liver
Species	Mouse
Lot	AF-2

reviewed by:
Verified Customer

Mouse

12/23/2014

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Summary

Application	Western Blot
Sample Tested	See PMID 23519696
Species	Mouse

reviewed by:
Angeliki Chroni

Human

11/03/2014

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Summary

Application	Immunofluorescence
Sample Tested	SK-N-SH human neuroblastoma cells
Species	Human

reviewed by:
Verified Customer

Rat

10/15/2012

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Summary

Application	Western Blot
Sample Tested	Cardiomyocytes from newborn rats
Species	Rat

reviewed by:
Cynthia Hong

Mouse

09/27/2011

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Summary

Application	Western Blot
Sample Tested	Mouse
Species	Mouse

reviewed by:
Verified Customer

Other

06/15/2009

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Summary

Application	Western Blot
Sample Tested	Rat astrocyte, Human Astrocyte, and Raw Mouse Neuron, Sample Amount: 25ug to 50ug
Species	Other

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View specific protocols for ABCA1 Antibody (NB400-105):

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FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 12 FAQs).

For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
- For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.
Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?
- Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.
We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?
- Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.
I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.
- Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.
To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?
- The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.
We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?
- ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.
Does this product contain any BSA?
- No, this antibody does not have any BSA in its formulation.
I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?
- The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.
Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?
- The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.
Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?
- To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.
Can i use DTT instead of Beta- mercaptoethanol?
- Either DTT or 2-mercaptoethanol is routinely used in SDS-PAGE sample buffer (reducing gel electrophoresis). Except that 2-mercaptoethanol is a stronger reducing agent and is mostly frequently used for that purpose.
Have you tested abca1 antibody(catalog # NB400-105) on hepatic Abca1 expression?
- We do not currently have any testing data where we have used NB400-105 with hepatic samples; all of our testing data for NB400-105 can be seen on the product page for this antibody. Should your customer be interested, we do show murine liver data with the following product: NB100-2068.

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Raw 264.7 Whole Cell - T0901317 treated Assay Kit
THP-1 TO901317 Treated / Untreated Cell Lysate

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Blogs on ABCA1. Showing 1-10 of 14 blog posts - Show all blog posts.

Check out the latest blog posts on ABCA1.

HIV-associated neurocognitive disorders involve extracellular Nef-induced modification of lipid rafts and redistribution of Alzheimer’s disease-related proteins
Jamshed Arslan, Pharm D, PhD Cholesterol is an essential part of animal cell membranes. Cholesterol-rich lipid rafts maintain the fluidity and protein trafficking of plasma membranes. Cellular ABCA1 protein moves cho... Read full blog post.

Phagocytes in Multiple Sclerosis: Myelin uptake leads to oxysterol-induced activation of liver X receptors, LXRs
By Jamshed Arslan Pharm.D., PhD.Myelin is a cholesterol-rich layer around nerves. Damage to this protective layer and the consequent slowing down of nerve impulses are hallmarks of multiple sclerosis (MS) and other ... Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis. Although cholesterol moves freely inside the cell, it cannot independently expo... Read full blog post.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr... Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp... Read full blog post.

ABCG8: Cholesterol's Fate
The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito... Read full blog post.

ABCA1
ABCA1 is a key gatekeeper influencing intracellular cholesterol transport, and is an important member of a multifamily of cAMP-dependent anion transporter cell membrane proteins that regulate reverse cholesterol efflux from cells in peripheral tissues... Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. ... Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP... Read full blog post.

LXR Alpha, ABCA1 and Cholesterol Homeostasis
LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee... Read full blog post.

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Bioinformatics

Gene Symbol	ABCA1
Entrez	19(Human) 11303(Mouse) 313210(Rat)
Uniprot	O95477(Human)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

ABCA1 Antibody - BSA Free

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