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ABCB11 Antibody (BSEP/7534) - Azide and BSA Free

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Western blot analysis of THP-1 cell lysate using ABCB11 Antibody (BSEP/7534) - Azide and BSA Free.
Formalin-fixed, paraffin-embedded human liver stained with ABCB11 Antibody (BSEP/7534) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
BSEP/7534
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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ABCB11 Antibody (BSEP/7534) - Azide and BSA Free Summary

Description
Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Immunogen
Recombinant fragment (around aa600-800) of human ABCB11 protein (exact sequence is proprietary)
Localization
Membrane
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
ABCB11
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT
  • Western Blot 1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for ABCB11 Antibody (BSEP/7534) - Azide and BSA Free

  • ABC member 16, MDR/TAP subfamily
  • ABC16
  • ATP-binding cassette sub-family B member 11
  • ATP-binding cassette, sub-family B (MDR/TAP), member 11
  • bile salt export pump
  • BSEP
  • BSEPPFIC2
  • EC 3.6.3
  • EC 3.6.3.44
  • PFIC-2
  • PGY4
  • progressive familial intrahepatic cholestasis 2
  • sister p-glycoprotein
  • SPGPBRIC2

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ABCB11