![Immunocytochemistry/Immunofluorescence: OATP2/8 Antibody (MDQ) [NB100-74482] - Analysis of OATP8 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a OATP8 monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. OATP8 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/OATP2-8-Antibody-MDQ-Immunocytochemistry-Immunofluorescence-NB100-74482-img0002.jpg "Immunocytochemistry/Immunofluorescence: OATP2/8 Antibody (MDQ) [NB100-74482] - Analysis of OATP8 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a OATP8 monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. OATP8 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | MDQ |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | This product was originally labeled as an antibody to OATP8. However, the amino acid sequence used as the immunizing peptide is 100% identical to OATP2. Furthermore, publications using this antibody show reactivity to both OATP2 and 8. As such, this antibody is reactive to both OATP2 and OATP8. |
| Immunogen | Synthetic peptide corresponding to residues M(1) D Q N Q H L N K T A E A Q P S E N K K T R Y(23) C of human OATP2. |
| Epitope | The exact epitope within the immunizing peptide sequence is unknown. |
| Specificity | NB100-74482 detects OATP2 and OATP8 from human tissue and cells. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SLCO1B1 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB usage w as reported in literature (See Cui Y et al.). IHC-P usage reported in scientific literature (PMID: 25063395). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tissue culture supernatant |
| Preservative | 0.09% Sodium Azide |
| Purity | Unpurified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: OATP2/8 Antibody (MDQ) [NB100-74482] - Immunohistochemical staining of liver transporters in the resected specimens from our case and control cases. Image collected and cropped by CiteAb from the following publication (surgicalcasereports.springeropen.com/articles/10.1186/s40792-016-0216-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OATP2-8-Antibody-MDQ-Immunohistochemistry-NB100-74482-img0003.jpg "Immunohistochemistry: OATP2/8 Antibody (MDQ) [NB100-74482] - Immunohistochemical staining of liver transporters in the resected specimens from our case and control cases. Image collected and cropped by CiteAb from the following publication (surgicalcasereports.springeropen.com/articles/10.1186/s40792-016-0216-8), licensed under a CC-BY license.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")