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5'-Nucleotidase/CD73 Antibody (606112) [mFluor Violet 500 SE]

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Product Details

Summary
Reactivity Hu, EqSpecies Glossary
Applications Flow, CyTOF-ready
Clone
606112
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 500 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB5795U-100UG
Alexa Fluor 405
FAB5795V-100UG
Alexa Fluor 488
FAB5795G-100UG
Alexa Fluor 532
FAB5795X
Alexa Fluor 594
FAB5795T-100UG
Alexa Fluor 647
FAB5795R-100UG
Alexa Fluor 700
FAB5795N-100UG
Alexa Fluor 750
FAB5795S-100UG
Allophycocyanin
FAB5795A
Allophycocyanin/Cy7
FAB5795APCCY7
Biotin
FAB5795B
CoraFluor 1
FAB5795CL1
DyLight 350
FAB5795D
DyLight 405
FAB5795E
DyLight 488
FAB5795K
DyLight 550
FAB5795L
DyLight 594
FAB5795M
DyLight 650
FAB5795W
DyLight 680
FAB5795Y
DyLight 755
FAB5795Z
FITC
FAB5795F
HRP
FAB5795H
Janelia Fluor 525
FAB5795JF525
Janelia Fluor 549
FAB5795I
Janelia Fluor 585
FAB5795JF585
Janelia Fluor 635
FAB5795JF635
Janelia Fluor 646
FAB5795J
Janelia Fluor 669
FAB5795JF669
PE/Atto594
FAB5795PEATT594
PE/Cy5.5
FAB5795PECY55
PE/Cy7
FAB5795PECY7
PerCP
FAB5795C
Phycoerythrin
FAB5795P
Unconjugated
MAB5795
mFluor Violet 450 SE
FAB5795MFV450
mFluor Violet 610 SE
FAB5795MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5795
Carrier Free
FAB5795A
Carrier Free
FAB5795P
Carrier Free
MAB5795
Carrier Free
MAB5795-SP
Carrier Free
FAB5795V-100UG
Carrier Free
FAB5795U-100UG
Carrier Free
FAB5795T-100UG
Carrier Free
FAB5795S-100UG
Carrier Free
FAB5795R-100UG
Carrier Free
FAB5795N-100UG
Carrier Free
FAB5795G-100UG
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5'-Nucleotidase/CD73 Antibody (606112) [mFluor Violet 500 SE] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human 5'‑Nucleotidase/CD73
Trp27-Ile511
Accession # P21589
Specificity
Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NT5E
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for 5'-Nucleotidase/CD73 Antibody (606112) [mFluor Violet 500 SE]

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for 5'-Nucleotidase/CD73 Antibody (FAB5795MFV500). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol NT5E