Caspase-8 Antibodies
We offer Caspase-8 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each Caspase-8 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Caspase-8 Antibodies can be used in a variety of model species: Bovine, Human, Monkey, Mouse, Primate, Rat. Use the list below to choose the Caspase-8 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Caspase-8 polyclonal antibodies and browse our Caspase-8 monoclonal antibody catalog.
Alternate Names for Caspase-8 Antibodies
anti-Caspase-8 antibody, anti-CASP8 antibody, anti-AIS antibody, anti-androgen receptor antibody, anti-DHTRTFM antibody, anti-Dihydrotestosterone receptorHYSP1 antibody, anti-HUMARA antibody, anti-NR3C4KD antibody, anti-Nuclear receptor subfamily 3 group C member 4 antibody, anti-SMAX1SBMA antibody, anti-spinal and bulbar muscular atrophy antibody
20 Results for "caspase-8" in Primary Antibodies
![Immunohistochemistry: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Reduced pancreatic Atg7 level increased the expression of Caspase-8 in 12-week-old Atg7deltapan mice. Caspase-8 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-8 (green) ( 1/1000, scale bar=50 um). Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-active-cleaved-Immunohistochemistry-NB100-56116-img0009.jpg "Immunohistochemistry: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Reduced pancreatic Atg7 level increased the expression of Caspase-8 in 12-week-old Atg7deltapan mice. Caspase-8 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-8 (green) ( 1/1000, scale bar=50 um). Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.")
![Western Blot: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-active-cleaved-Western-Blot-NB100-56116-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tumor tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Anaplastic glioma (Grade III, left) and Gemistocytoma (Grade II, right) cores showing negative and positive staining for Caspase-8, respectively. B. Higher magnification of the Gemistocytoma tumor (from A).](http://images.novusbio.com/fullsize/Caspase-8-Antibody-active-cleaved-Immunohistochemistry-Paraffin-NB100-56116-img0004.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of cortex (from A). C. Higher magnification of brain stem (from A). D. Normal cerebellum showing caspase-8 staining in the Purkinge cells.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-active-cleaved-Immunohistochemistry-Paraffin-NB100-56116-img0005.jpg)
![Immunohistochemistry: Caspase-8 Antibody [NB100-56116] - Necroptosis marker p-MLKL can be detected in OA cartilage & after TNF/CHX stimulation.Exemplary images of impacted & differently stimulated cartilage explants after immunohistochemical staining of a cleaved CASP8, b RIPK3, & c p-MLKL. In addition, percentage of cleaved CASP8− (d) & p-MLKL− (e) positive cells was quantified (n = 3). Images were acquired using a 20× objective; the bars represent 200 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527653), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56116_rabbit-polyclonal-caspase-8-antibody-active-cleaved-imgenex-img-5703-310202415392576.jpg)
![Immunohistochemistry: Caspase-8 Antibody [NB100-56116] - Expression of necroptosis- & apoptosis-related markers is elevated in OA cartilage.Necroptosis- & apoptosis-related markers in highly degenerated cartilage tissue of OA patients (ICRS grade ≥ 3) were determined by a gene expression analysis of apoptosis & necroptosis markers as well as immunohistochemical analysis (IHC) of b cleaved CASP8, c RIPK3, & d p-MLKL. Macroscopically intact cartilage (ICRS grade ≤ 1) served as control. Bars in the IHC images represent 200 µm. Statistical analysis was performed by an unpaired multiple t test; error bars indicate median & range from min to max; n = 13. Significant differences between groups were depicted as: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527653), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56116_rabbit-polyclonal-caspase-8-antibody-active-cleaved-imgenex-img-5703-310202415394549.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NB100-56116] - Analysis of the cell type susceptible to apoptotic cell death in the hypothalamus of IRS2−/− mice. Immunoblots probed with antibodies against GFAP (A) & Tuj-1 (B) in the hypothalamus of wild-type (WT), non-diabetic IRS2-deficient (ND IRS2−/−) & diabetic IRS2-deficient (D IRS2−/−) mice. The average of three independent assays performed in duplicate is shown. Statistical significance by ANOVA: *P<0.05 vs WT mice. n=6/group. (C) Colocalization of TUNEL & GFAP in the hypothalamus of WT, ND IRS2−/− & D IRS2−/− mice. Arrows indicate astrocytes with apoptotic nuclei. (D) Colocalization of cleaved caspase-8 & GFAP, & of cleaved caspase-8 & NeuN, in the hypothalamus of WT, ND IRS2−/− & D IRS2−/− mice. Arrows indicate colocalization of GFAP & cleaved caspase-8. Scale bar: 50 µm; inset, 100 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/dmm/article/doi/10.1242/dmm.023515/257174/Increased-oxidative-stress-and-apoptosis-in-the), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56116_rabbit-polyclonal-caspase-8-antibody-active-cleaved-imgenex-img-5703-31020241683918.jpg)
![Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0018.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Caspase-8 expression in 786-O and A-498 cellsClose](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0022.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody (90A992) [NB100-56527] - Formalin-fixed, paraffin-embedded human spleen (top) and breast (bottom) stained with Caspase-8 antibody at 4 ug/ml. Localization can be cytoplasmic and nuclear. Cancer/normal adjacent tissue array was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Immunohistochemistry-Paraffin-NB100-56527-img0014.jpg)
![Flow Cytometry: Caspase-8 Antibody (90A992) [NB100-56527] - Flow cytometric analysis of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody was used for this test. IC-Flow (Intracellular Staining Flow Cytometry Kit) was used to fix and prepare the cells for staining.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Flow-Cytometry-NB100-56527-img0008.jpg)
![Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Analysis using the Biotin conjugate of NB100-56527. Detection of human Caspase-8 using Jurkat lysates with NB100-55786 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. NB100-55786 only detects 55 kDa Caspase-8 in Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0021.jpg)
![Simple Western: Caspase-8 Antibody (90A992) [NB100-56527] - Simple Western lane view shows a specific band for Caspase 8 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Simple-Western-NB100-56527-img0019.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, and caspases 3, 8, and 9) and inflammation (TNF-a) in the heart of hypertensive rats (A) SHR and (B) DOCA-salt, and rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected and cropped by Citeab from the following publication (The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension. Molecules (2018) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Pro-and-Active-Western-Blot-NBP1-05123-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, and caspases 3, 8, and 9) and inflammation (TNF-a) in the heart of hypertensive rats (A) SHR and (B) DOCA-salt, and rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected and cropped by Citeab from the following publication (The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension. Molecules (2018) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using NBP1-05123 at 1:2000. A. Anaplastic glioma cores from two different patients, positive (left) and negative (right) staining for caspase-8. B. Higher magnification from the caspase-8 positive (A, left) core.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Pro-and-Active-Immunohistochemistry-Paraffin-NBP1-05123-img0002.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - AML12 whole cell lysate. Good specific band is observed. Primary antibody at 1: 2000, secondary antibody at 1: 20000. Skimmed milk as blocking buffer. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Pro-and-Active-Western-Blot-NBP1-05123-img0003.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05123_rabbit-polyclonal-caspase-8-antibody-pro-and-active-31020241534352.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05123_rabbit-polyclonal-caspase-8-antibody-pro-and-active-310202415394532.jpg)
![Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05123_rabbit-polyclonal-caspase-8-antibody-pro-and-active-31020241535665.jpg)
![Knockout Validated: Caspase-8 Antibody [NBP2-15722] - Wild-type (WT) and Caspase 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP2-15722-img0005.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Caspase-8 Antibody [NBP2-15722] - Wild-type (WT) and Caspase 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP2-15722-img0015.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP2-15722-img0008.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP2-15722-img0012.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase-8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242015729.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242001016.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: Caspase-8 protein stained by Caspase-8 antibody (NBP2-15722) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242063388.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:100.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242001090.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242021975.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase-8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-910202420122084.jpg)
![Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP1-76610-img0006.jpg "Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76610-img0003.jpg)
![Immunohistochemistry: Caspase-8 Antibody [NBP1-76610] - Staining of human spleen tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunohistochemistry-NBP1-76610-img0004.jpg)
![Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis in Jurkat cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP1-76610-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Jurkat cells with caspase-8 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76610-img0002.jpg)
![Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - IF of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 ug/ml. Red: Caspase-8 Antibody (NBP1-76610). Blue: DAPI staining](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunofluorescence-NBP1-76610-img0007.jpg)
![Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208] - CaSki cells were mock-infected or exposed to each of the indicated viruses (MOI ~ 1 pfu/cell) or 10 uM staurosporine and 4 h pi, cellular lysates were prepared and analyzed by immunoblotting for Caspase-8. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006766) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-FLICE-4-1-20-Western-Blot-NB500-208-img0004.jpg "Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208] - CaSki cells were mock-infected or exposed to each of the indicated viruses (MOI ~ 1 pfu/cell) or 10 uM staurosporine and 4 h pi, cellular lysates were prepared and analyzed by immunoblotting for Caspase-8. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006766) licensed under a CC-BY license.")
![Western Blot: Caspase 8 Antibody (FLICE 4-1-20) [NB500-208] - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using NB500-208 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-FLICE-4-1-20-Western-Blot-NB500-208-img0003.jpg)
![Western Blot: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis using Caspase 8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-Western-Blot-NBP2-22183-img0005.jpg "Western Blot: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis using Caspase 8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of liver cancer tissues using Caspase 8 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-Immunohistochemistry-Paraffin-NBP2-22183-img0003.jpg)
![Flow Cytometry: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of NIH/3T3 cells using Caspase 8 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-Flow-Cytometry-NBP2-22183-img0001.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of colon cancer tissues using Caspase 8 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-Immunohistochemistry-Paraffin-NBP2-22183-img0002.jpg)
![ELISA: Caspase-8 Antibody (1H11) [NBP2-22183] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-ELISA-NBP2-22183-img0004.jpg)
![Western Blot: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 3: Jurkat treated with 25uM Etoposide for 16 hours cell lysate Lane 4: Jurkat treated with 1uM staurosporine for 3 hours cell lysate Lane 5: HeLa cell lysate Lane 6: HeLa treated with 1uM staurosporine for 3 hours cell lysate Lane 7: HeLa treated with 100uM Etoposide for 4 hours cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 55 kDa Observed band size: 18-55 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32109) at 1/2,000 dilution was used in antibody diluent at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32109_rabbit-caspase-8-mab-psh04-77-278202416285279.jpg "Western Blot: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 3: Jurkat treated with 25uM Etoposide for 16 hours cell lysate Lane 4: Jurkat treated with 1uM staurosporine for 3 hours cell lysate Lane 5: HeLa cell lysate Lane 6: HeLa treated with 1uM staurosporine for 3 hours cell lysate Lane 7: HeLa treated with 100uM Etoposide for 4 hours cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 55 kDa Observed band size: 18-55 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32109) at 1/2,000 dilution was used in antibody diluent at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32109) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32109_rabbit-caspase-8-mab-psh04-77-298202414161856.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Immunocytochemistry analysis of HeLa cells treated with or without 1μM staurosporine for 3 hours labeling Caspase-8 with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-8 antibody (NBP3-32109) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32109_rabbit-caspase-8-mab-psh04-77-29202410234224.jpg)
![Flow Cytometry: Caspase-8 Antibody (PSH04-77) [NBP3-32109] - Flow cytometric analysis of HeLa cells treated with or without 1μM staurosporine for 3 hours cells labeling Caspase-8. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32109, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32109_rabbit-caspase-8-mab-psh04-77-29202416453297.jpg)
![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0004.jpg "Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.")
![Flow Cytometry: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using Caspase-8 mAbs (3B10) (Prod. No. NBP2-80098)](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Flow-Cytometry-NBP2-80098-img0001.jpg)
![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0002.jpg)
![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0003.jpg)