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Caspase-8 Antibody (90A992) [DyLight 650]

 
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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clone
90A992
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56527AF350
Alexa Fluor 405
NB100-56527AF405
Alexa Fluor 488
NB100-56527AF488
Alexa Fluor 532
NB100-56527AF532
Alexa Fluor 594
NB100-56527AF594
Alexa Fluor 647
NB100-56527AF647
Alexa Fluor 700
NB100-56527AF700
Alexa Fluor 750
NB100-56527AF750
Allophycocyanin
NB100-56527APC
Biotin
NB100-56527B
Biotin
NB100-55786
DyLight 350
NB100-56527UV
DyLight 405
NB100-56527V
DyLight 488
NB100-56527G
DyLight 550
NB100-56527R
DyLight 594
NB100-56527DL594
DyLight 680
NB100-56527FR
DyLight 755
NB100-56527IR
FITC
NB100-56527F
HRP
NB100-56527H
Janelia Fluor 549
NB100-56527JF549
Janelia Fluor 646
NB100-56527JF646
PE
NB100-56527PE
PerCP
NB100-56527PCP
Unconjugated
NB100-56527
mFluor Violet 450 SE
NB100-56527MFV450
mFluor Violet 500 SE
NB100-56527MFV500
mFluor Violet 610 SE
NB100-56527MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56527
BSA Free
NB100-56527
BSA Free
NB100-56527SS
Azide and BSA Free
NBP2-80616
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Caspase-8 Antibody (90A992) [DyLight 650] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
The immunogen for this product falls between amino acids 360-385 of the human Caspase 8 protein.
Specificity
This can be used for detection of the pro-form of Caspase-8.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CASP8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Chimpanzee and Rhesus Monkey reactivity noted.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Caspase-8 Antibody (90A992) [DyLight 650]

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-8 Antibody (NB100-56527C). (Showing 1 - 1 of 1 FAQ).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Secondary Antibodies

 

Isotype Controls

Additional Caspase-8 Products

Research Areas for Caspase-8 Antibody (NB100-56527C)

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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
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The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.
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Bioinformatics

Gene Symbol CASP8