![Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Azide-and-BSA-Free-Western-Blot-NBP2-80616-img0003.jpg "Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, Simple Western, Flow, IHC, CyTOF-ready |
| Clone | 90A992 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | The immunogen for this product falls between amino acids 360-385 of the human Caspase 8 protein. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CASP8 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. This antibody is CyTOF ready. |
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| Control |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Caspase-8 Antibody (NBP2-80616)Find related products by research area.
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The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ... Read full blog post. |
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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T... Read full blog post. |
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Caspase-3, The Executioner of Apoptosis The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat... Read full blog post. |
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VPS41 - An important regulator of lysosomal trafficking Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab... Read full blog post. |
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Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req... Read full blog post. |
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Caspase 8 - a key mediator of apoptosis Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a... Read full blog post. |
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| Gene Symbol | CASP8 |
![Immunohistochemistry-Paraffin: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Formalin-fixed, paraffin-embedded human spleen (top) and breast (bottom) stained with Caspase-8 antibody at 4 ug/ml. Localization can be cytoplasmic and nuclear. Cancer/normal adjacent tissue array was used for this test. Staining of formalin-fixed tissue](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80616-img0004.jpg "Immunohistochemistry-Paraffin: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Formalin-fixed, paraffin-embedded human spleen (top) and breast (bottom) stained with Caspase-8 antibody at 4 ug/ml. Localization can be cytoplasmic and nuclear. Cancer/normal adjacent tissue array was used for this test. Staining of formalin-fixed tissue")
![Flow Cytometry: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Flow cytometric analysis of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody was used for this test. IC-Flow (Intracellular Staining Flow Cytometry Kit) was used to fix and prepare the cells for staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80616-img0005.jpg "Flow Cytometry: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Flow cytometric analysis of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody was used for this test. IC-Flow (Intracellular Staining Flow Cytometry Kit) was used to fix and prepare the cells for staining. Image from the standard format of this antibody.")
![Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Analysis using the Biotin conjugate of NB100-56527. Detection of human Caspase-8 using Jurkat lysates with NB100-55786 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. NB100-55786 only detects 55 kDa Caspase-8 in Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Azide-and-BSA-Free-Western-Blot-NBP2-80616-img0001.jpg "Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Analysis using the Biotin conjugate of NB100-56527. Detection of human Caspase-8 using Jurkat lysates with NB100-55786 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. NB100-55786 only detects 55 kDa Caspase-8 in Jurkat cells.")
![Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Caspase-8 expression in 786-O and A-498 cellsClose Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Azide-and-BSA-Free-Western-Blot-NBP2-80616-img0002.jpg "Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Caspase-8 expression in 786-O and A-498 cellsClose Image from the standard format of this antibody.")
![Simple Western: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Simple Western lane view shows a specific band for Caspase 8 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Azide-and-BSA-Free-Simple-Western-NBP2-80616-img0006.jpg "Simple Western: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Simple Western lane view shows a specific band for Caspase 8 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")