![Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208] - CaSki cells were mock-infected or exposed to each of the indicated viruses (MOI ~ 1 pfu/cell) or 10 uM staurosporine and 4 h pi, cellular lysates were prepared and analyzed by immunoblotting for Caspase-8. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006766) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-FLICE-4-1-20-Western-Blot-NB500-208-img0004.jpg "Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208] - CaSki cells were mock-infected or exposed to each of the indicated viruses (MOI ~ 1 pfu/cell) or 10 uM staurosporine and 4 h pi, cellular lysates were prepared and analyzed by immunoblotting for Caspase-8. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006766) licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB |
| Clone | FLICE 4-1-20 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Full-length recombinant human Caspase 8 [UniProt# Q14790] |
| Localization | Cytoplasmic |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CASP8 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This Caspase 8 (FLICE 4-1-20) antibody is useful for Western Blot where a band is seen at 55 kDa representing the inactive form of Caspase 8 (see protocol for additional information). We also have reports that the antibody detects the active (pro) form of Caspase 8. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Ascites |
| Preservative | 0.1% Sodium Azide |
| Purity | Unpurified |
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| 293 Whole Cell Lysate | |
| Caspase-8 Overexpression Lysate | |
| Jurkat Staurosporine Treated / Untreated Cell Lysate |
Secondary Antibodies |
Isotype Controls |
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Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req... Read full blog post. |
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![Western Blot: Caspase 8 Antibody (FLICE 4-1-20) [NB500-208] - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using NB500-208 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-FLICE-4-1-20-Western-Blot-NB500-208-img0003.jpg "Western Blot: Caspase 8 Antibody (FLICE 4-1-20) [NB500-208] - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using NB500-208 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.")
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