![Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP1-76610-img0006.jpg "Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 ug/ml.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A. The immunogen is located within the last 50 amino acids of Caspase-8. Amino Acid Squence: NYEVSNKDDKKNMGKQ |
| Specificity | Depending on cell lines or tissues used, either full-length or other cleavage products may be observed. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CASP8 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Western Blot FADD [p Ser194] Antibody (709736)](https://images.novusbio.com/images2/FADD_MAB7047_Western_Blot_11404.jpg)
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| Publication using NBP1-76610 | Applications | Species |
|---|---|---|
| Marwarha G, Rosand o, Scrimgeour N et al. miR-210 Regulates Apoptotic Cell Death during Cellular Hypoxia and Reoxygenation in a Diametrically Opposite Manner Biomedicines 2021-12-25 [PMID: 35052722] (ELISA) | ELISA |
Secondary Antibodies |
Isotype Controls |
Research Areas for Caspase-8 Antibody (NBP1-76610)Find related products by research area.
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The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ... Read full blog post. |
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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T... Read full blog post. |
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Caspase-3, The Executioner of Apoptosis The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat... Read full blog post. |
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VPS41 - An important regulator of lysosomal trafficking Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab... Read full blog post. |
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Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req... Read full blog post. |
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Caspase 8 - a key mediator of apoptosis Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76610-img0003.jpg "Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 ug/mL.")
![Immunohistochemistry: Caspase-8 Antibody [NBP1-76610] - Staining of human spleen tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunohistochemistry-NBP1-76610-img0004.jpg "Immunohistochemistry: Caspase-8 Antibody [NBP1-76610] - Staining of human spleen tissue with antibody at 5 ug/mL.")
![Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis in Jurkat cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP1-76610-img0005.jpg "Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis in Jurkat cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Jurkat cells with caspase-8 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76610-img0002.jpg "Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Jurkat cells with caspase-8 antibody at 2 ug/ml.")
![Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - IF of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 ug/ml. Red: Caspase-8 Antibody (NBP1-76610). Blue: DAPI staining](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunofluorescence-NBP1-76610-img0007.jpg "Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - IF of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 ug/ml. Red: Caspase-8 Antibody (NBP1-76610). Blue: DAPI staining")
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610] - Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76610_rabbit-polyclonal-caspase-8-antibody-2711202419592074.jpg "Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610] - Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 u/mL.")
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610] - Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76610_rabbit-polyclonal-caspase-8-antibody-271120241932684.jpg "Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610] - Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 u/mL.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")