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Caspase-8 Antibody (1G12) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
1G12
Clonality
Monoclonal
Host
Rat
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-80097AF350
Alexa Fluor 405
NBP2-80097AF405
Alexa Fluor 488
NBP2-80097AF488
Alexa Fluor 532
NBP2-80097AF532
Alexa Fluor 594
NBP2-80097AF594
Alexa Fluor 647
NBP2-80097AF647
Alexa Fluor 700
NBP2-80097AF700
Alexa Fluor 750
NBP2-80097AF750
Allophycocyanin
NBP2-80097APC
Biotin
NBP2-80097B
CoraFluor 1
NBP2-80097CL1
DyLight 350
NBP2-80097UV
DyLight 405
NBP2-80097V
DyLight 488
NBP2-80097G
DyLight 550
NBP2-80097R
DyLight 594
NBP2-80097DL594
DyLight 650
NBP2-80097C
DyLight 680
NBP2-80097FR
DyLight 755
NBP2-80097IR
FITC
NBP2-80097F
HRP
NBP2-80097H
Janelia Fluor 525
NBP2-80097JF525
Janelia Fluor 549
NBP2-80097JF549
Janelia Fluor 585
NBP2-80097JF585
Janelia Fluor 635
NBP2-80097JF635
Janelia Fluor 646
NBP2-80097JF646
PE
NBP2-80097PE
PerCP
NBP2-80097PCP
Unconjugated
NBP2-80097
mFluor Violet 450 SE
NBP2-80097MFV450
mFluor Violet 500 SE
NBP2-80097MFV500
mFluor Violet 610 SE
NBP2-80097MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-80097
BSA Free
NBP2-80097
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Caspase-8 Antibody (1G12) [Janelia Fluor® 669] Summary

Immunogen
Recombinant mouse caspase-8 (p20 subunit).
Specificity
Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
CASP8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Caspase-8 Antibody (1G12) [Janelia Fluor® 669]

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-8 Antibody (NBP2-80097JF669). (Showing 1 - 1 of 1 FAQ).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Caspase-8 Antibody (NBP2-80097JF669)

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Blogs on Caspase-8.

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By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.
Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
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The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.
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Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
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Bioinformatics

Gene Symbol CASP8